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Comparison of antimicrobial effect of several decontaminating methods on contaminated Titanium discs

BACKGROUND: Decontaminating the implant surface, exposed to bacterial biofilm, is a concern in the treatment of peri-implant inflammatory disease. The aim of this study was to compare the effect of several methods on reduction of the bacterial load, colonized on the surfaces of titanium discs. MATER...

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Autores principales: Birang, Reza, Shakerian, Kourosh, Birang, Ehsan, Narimani, Tahmineh, Naghsh, Narges, Fekrazad, Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9807942/
https://www.ncbi.nlm.nih.gov/pubmed/36605136
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author Birang, Reza
Shakerian, Kourosh
Birang, Ehsan
Narimani, Tahmineh
Naghsh, Narges
Fekrazad, Reza
author_facet Birang, Reza
Shakerian, Kourosh
Birang, Ehsan
Narimani, Tahmineh
Naghsh, Narges
Fekrazad, Reza
author_sort Birang, Reza
collection PubMed
description BACKGROUND: Decontaminating the implant surface, exposed to bacterial biofilm, is a concern in the treatment of peri-implant inflammatory disease. The aim of this study was to compare the effect of several methods on reduction of the bacterial load, colonized on the surfaces of titanium discs. MATERIALS AND METHODS: In this in vivo study, seven titanium discs with Sandblasted, Large-grit, acid-etched (SLA) surface were placed in the mouth of each of ten patients with chronic periodontitis by an intra-oral maxillary splint for 24 h. In each patient, the contaminated discs, except for the negative control ones, were randomly treated by one of the six antiseptic methods including sterile normal saline, plastic curette, air polisher, hydrogen peroxide, 980 nm diode laser, and Er-YAG laser. A spectrophotometer was used to measure Optical Density (OD) in case of aerobic microorganisms. Colony-Forming Units (CFUs) were used for anaerobic bacteria. Data were analyzed through Kruskal–Wallis and Mann–Whitney Tests at a significance level of α =0.05 by SPSS software. RESULTS: Statistical analysis showed a significant decrease in OD of aerobic bacteria among the seven groups during a 0–24 h time interval (P < 0.001). Furthermore, these tests showed a significant difference in the CFU (P < 0.001) for anaerobic bacteria after 48 h. CONCLUSION: The results of this study showed that all of the adopted methods significantly reduced microbial colonies on the surfaces of titanium discs with SLA surface. Er: YAG laser and normal saline had the highest and the lowest effects, respectively.
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spelling pubmed-98079422023-01-04 Comparison of antimicrobial effect of several decontaminating methods on contaminated Titanium discs Birang, Reza Shakerian, Kourosh Birang, Ehsan Narimani, Tahmineh Naghsh, Narges Fekrazad, Reza Dent Res J (Isfahan) Original Article BACKGROUND: Decontaminating the implant surface, exposed to bacterial biofilm, is a concern in the treatment of peri-implant inflammatory disease. The aim of this study was to compare the effect of several methods on reduction of the bacterial load, colonized on the surfaces of titanium discs. MATERIALS AND METHODS: In this in vivo study, seven titanium discs with Sandblasted, Large-grit, acid-etched (SLA) surface were placed in the mouth of each of ten patients with chronic periodontitis by an intra-oral maxillary splint for 24 h. In each patient, the contaminated discs, except for the negative control ones, were randomly treated by one of the six antiseptic methods including sterile normal saline, plastic curette, air polisher, hydrogen peroxide, 980 nm diode laser, and Er-YAG laser. A spectrophotometer was used to measure Optical Density (OD) in case of aerobic microorganisms. Colony-Forming Units (CFUs) were used for anaerobic bacteria. Data were analyzed through Kruskal–Wallis and Mann–Whitney Tests at a significance level of α =0.05 by SPSS software. RESULTS: Statistical analysis showed a significant decrease in OD of aerobic bacteria among the seven groups during a 0–24 h time interval (P < 0.001). Furthermore, these tests showed a significant difference in the CFU (P < 0.001) for anaerobic bacteria after 48 h. CONCLUSION: The results of this study showed that all of the adopted methods significantly reduced microbial colonies on the surfaces of titanium discs with SLA surface. Er: YAG laser and normal saline had the highest and the lowest effects, respectively. Wolters Kluwer - Medknow 2022-12-14 /pmc/articles/PMC9807942/ /pubmed/36605136 Text en Copyright: © 2022 Dental Research Journal https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Birang, Reza
Shakerian, Kourosh
Birang, Ehsan
Narimani, Tahmineh
Naghsh, Narges
Fekrazad, Reza
Comparison of antimicrobial effect of several decontaminating methods on contaminated Titanium discs
title Comparison of antimicrobial effect of several decontaminating methods on contaminated Titanium discs
title_full Comparison of antimicrobial effect of several decontaminating methods on contaminated Titanium discs
title_fullStr Comparison of antimicrobial effect of several decontaminating methods on contaminated Titanium discs
title_full_unstemmed Comparison of antimicrobial effect of several decontaminating methods on contaminated Titanium discs
title_short Comparison of antimicrobial effect of several decontaminating methods on contaminated Titanium discs
title_sort comparison of antimicrobial effect of several decontaminating methods on contaminated titanium discs
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9807942/
https://www.ncbi.nlm.nih.gov/pubmed/36605136
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