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Interference with orco gene expression affects host recognition in Diorhabda tarsalis

Introduction: Diorhabda tarsalis Weise is an important insect pest of the Chinese licorice Glycyrrhiza uralensis Fisch. Behavior of the beetle, including host location, oviposition site selection, self-defense, and aggregation, were regulated by plant volatiles or insect pheromones. Aim: In this stu...

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Autores principales: Chen, Hong-Hao, Dewer, Youssef, Wang, Yan, Tan, Shu-Qian, Liu, Xiao-Li, Shi, Wang-Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9808408/
https://www.ncbi.nlm.nih.gov/pubmed/36605899
http://dx.doi.org/10.3389/fphys.2022.1069391
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author Chen, Hong-Hao
Dewer, Youssef
Wang, Yan
Tan, Shu-Qian
Liu, Xiao-Li
Shi, Wang-Peng
author_facet Chen, Hong-Hao
Dewer, Youssef
Wang, Yan
Tan, Shu-Qian
Liu, Xiao-Li
Shi, Wang-Peng
author_sort Chen, Hong-Hao
collection PubMed
description Introduction: Diorhabda tarsalis Weise is an important insect pest of the Chinese licorice Glycyrrhiza uralensis Fisch. Behavior of the beetle, including host location, oviposition site selection, self-defense, and aggregation, were regulated by plant volatiles or insect pheromones. Aim: In this study, Identification of ORs and function research on orco were carried out, these could lead to the development of understand for olfaction mechanism in D. tarsalis. Methods: ORs were identified by PacBio RS II platform to sequence the antennas of adult D. tarsalis, the function of orco was explored by dsRNA interference. Results: 29 odorant receptor candidate genes of D. tarsalis were obtained, which code for 130–479 amino acids. Phylogenetic trees of olfactory receptors were constructed with 243 ORs from eight Coleoptera species. DtarORco, DtarOR7 and DtarOR26 are specifically expressed in the antenna, and the expression levels were significantly higher than other DtarORs in antenna, there were no differential expression between male and female beetles. An odorant coreceptor gene (DtarORco) has characteristics of an odorant receptor family member, the encoded mature protein has a predicted molecular weight of 53.898 kDa, dsRNA L4440 expression vectors were constructed and successfully transformed into ribonuclease III-deficient Escherichia coli strain HT115 DE3. After interference treatment, the relative expression level of DtarORco in D. tarsalis antennae significantly decreased and electrophysiological responses to host localization odor signals significantly decreased. At the same time, beetles lost the ability to locate hosts. Discussion: The research on its mechanism of olfaction may lead to the development of new control measures that are environmentally friendly.
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spelling pubmed-98084082023-01-04 Interference with orco gene expression affects host recognition in Diorhabda tarsalis Chen, Hong-Hao Dewer, Youssef Wang, Yan Tan, Shu-Qian Liu, Xiao-Li Shi, Wang-Peng Front Physiol Physiology Introduction: Diorhabda tarsalis Weise is an important insect pest of the Chinese licorice Glycyrrhiza uralensis Fisch. Behavior of the beetle, including host location, oviposition site selection, self-defense, and aggregation, were regulated by plant volatiles or insect pheromones. Aim: In this study, Identification of ORs and function research on orco were carried out, these could lead to the development of understand for olfaction mechanism in D. tarsalis. Methods: ORs were identified by PacBio RS II platform to sequence the antennas of adult D. tarsalis, the function of orco was explored by dsRNA interference. Results: 29 odorant receptor candidate genes of D. tarsalis were obtained, which code for 130–479 amino acids. Phylogenetic trees of olfactory receptors were constructed with 243 ORs from eight Coleoptera species. DtarORco, DtarOR7 and DtarOR26 are specifically expressed in the antenna, and the expression levels were significantly higher than other DtarORs in antenna, there were no differential expression between male and female beetles. An odorant coreceptor gene (DtarORco) has characteristics of an odorant receptor family member, the encoded mature protein has a predicted molecular weight of 53.898 kDa, dsRNA L4440 expression vectors were constructed and successfully transformed into ribonuclease III-deficient Escherichia coli strain HT115 DE3. After interference treatment, the relative expression level of DtarORco in D. tarsalis antennae significantly decreased and electrophysiological responses to host localization odor signals significantly decreased. At the same time, beetles lost the ability to locate hosts. Discussion: The research on its mechanism of olfaction may lead to the development of new control measures that are environmentally friendly. Frontiers Media S.A. 2022-12-20 /pmc/articles/PMC9808408/ /pubmed/36605899 http://dx.doi.org/10.3389/fphys.2022.1069391 Text en Copyright © 2022 Chen, Dewer, Wang, Tan, Liu and Shi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Chen, Hong-Hao
Dewer, Youssef
Wang, Yan
Tan, Shu-Qian
Liu, Xiao-Li
Shi, Wang-Peng
Interference with orco gene expression affects host recognition in Diorhabda tarsalis
title Interference with orco gene expression affects host recognition in Diorhabda tarsalis
title_full Interference with orco gene expression affects host recognition in Diorhabda tarsalis
title_fullStr Interference with orco gene expression affects host recognition in Diorhabda tarsalis
title_full_unstemmed Interference with orco gene expression affects host recognition in Diorhabda tarsalis
title_short Interference with orco gene expression affects host recognition in Diorhabda tarsalis
title_sort interference with orco gene expression affects host recognition in diorhabda tarsalis
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9808408/
https://www.ncbi.nlm.nih.gov/pubmed/36605899
http://dx.doi.org/10.3389/fphys.2022.1069391
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