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miRNA profiling in intrauterine exosomes of pregnant cattle on day 7
Intrauterine exosomes have been identified to be involved in the embryo development and implantation. The aim of this study was to explore the role of miRNAs in intrauterine exosomes in bovine pregnancy. Intrauterine exosomes were collected from uterine flushing fluids of three donor and three recip...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9810022/ https://www.ncbi.nlm.nih.gov/pubmed/36605764 http://dx.doi.org/10.3389/fvets.2022.1078394 |
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author | Zhai, Yaying Shi, Qiaoting Chu, Qiuxia Chen, Fuying Feng, Yajie Zhang, Zijing Qi, Xinglei Arends, Danny Brockmann, Gudrun A. Wang, Eryao Lyu, Shijie |
author_facet | Zhai, Yaying Shi, Qiaoting Chu, Qiuxia Chen, Fuying Feng, Yajie Zhang, Zijing Qi, Xinglei Arends, Danny Brockmann, Gudrun A. Wang, Eryao Lyu, Shijie |
author_sort | Zhai, Yaying |
collection | PubMed |
description | Intrauterine exosomes have been identified to be involved in the embryo development and implantation. The aim of this study was to explore the role of miRNAs in intrauterine exosomes in bovine pregnancy. Intrauterine exosomes were collected from uterine flushing fluids of three donor and three recipient Xianan cows 7 days after fertilization. Intrauterine exosomes miRNAs were extracted and the exosomal miRNAs expression levels were analyzed. Sixty miRNAs differed significantly in their amounts between donors and recipients (p-value < 0.05, |log2(FoldChange)| > 1). Twenty-two miRNAs were upregulated and 38 downregulated in the group of donor cows. The bta-miR-184 was the most significant (P(Benjamini-Hochberg) < 0.001). A total of 9,775 target genes were predicted using the 60 miRNAs. GO and KEGG analysis showed that the target genes were enriched in several biological processes or pathways associated with embryo implantation and endometrial development, such as cell adhesion, cell junction, focal adhesion, and Rap1 signaling pathway. Our findings suggest that, in cattle early pregnancy stage, these differently expressed miRNAs in intrauterine exosomes involved in embryo implantation and endometrial development, which may exert a significant effect and influence the uterine microenvironment for embryo implantation. These results could provide reference for screening and exploring the intrauterine exosomal miRNA affecting embryo implantation. |
format | Online Article Text |
id | pubmed-9810022 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98100222023-01-04 miRNA profiling in intrauterine exosomes of pregnant cattle on day 7 Zhai, Yaying Shi, Qiaoting Chu, Qiuxia Chen, Fuying Feng, Yajie Zhang, Zijing Qi, Xinglei Arends, Danny Brockmann, Gudrun A. Wang, Eryao Lyu, Shijie Front Vet Sci Veterinary Science Intrauterine exosomes have been identified to be involved in the embryo development and implantation. The aim of this study was to explore the role of miRNAs in intrauterine exosomes in bovine pregnancy. Intrauterine exosomes were collected from uterine flushing fluids of three donor and three recipient Xianan cows 7 days after fertilization. Intrauterine exosomes miRNAs were extracted and the exosomal miRNAs expression levels were analyzed. Sixty miRNAs differed significantly in their amounts between donors and recipients (p-value < 0.05, |log2(FoldChange)| > 1). Twenty-two miRNAs were upregulated and 38 downregulated in the group of donor cows. The bta-miR-184 was the most significant (P(Benjamini-Hochberg) < 0.001). A total of 9,775 target genes were predicted using the 60 miRNAs. GO and KEGG analysis showed that the target genes were enriched in several biological processes or pathways associated with embryo implantation and endometrial development, such as cell adhesion, cell junction, focal adhesion, and Rap1 signaling pathway. Our findings suggest that, in cattle early pregnancy stage, these differently expressed miRNAs in intrauterine exosomes involved in embryo implantation and endometrial development, which may exert a significant effect and influence the uterine microenvironment for embryo implantation. These results could provide reference for screening and exploring the intrauterine exosomal miRNA affecting embryo implantation. Frontiers Media S.A. 2022-12-20 /pmc/articles/PMC9810022/ /pubmed/36605764 http://dx.doi.org/10.3389/fvets.2022.1078394 Text en Copyright © 2022 Zhai, Shi, Chu, Chen, Feng, Zhang, Qi, Arends, Brockmann, Wang and Lyu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Zhai, Yaying Shi, Qiaoting Chu, Qiuxia Chen, Fuying Feng, Yajie Zhang, Zijing Qi, Xinglei Arends, Danny Brockmann, Gudrun A. Wang, Eryao Lyu, Shijie miRNA profiling in intrauterine exosomes of pregnant cattle on day 7 |
title | miRNA profiling in intrauterine exosomes of pregnant cattle on day 7 |
title_full | miRNA profiling in intrauterine exosomes of pregnant cattle on day 7 |
title_fullStr | miRNA profiling in intrauterine exosomes of pregnant cattle on day 7 |
title_full_unstemmed | miRNA profiling in intrauterine exosomes of pregnant cattle on day 7 |
title_short | miRNA profiling in intrauterine exosomes of pregnant cattle on day 7 |
title_sort | mirna profiling in intrauterine exosomes of pregnant cattle on day 7 |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9810022/ https://www.ncbi.nlm.nih.gov/pubmed/36605764 http://dx.doi.org/10.3389/fvets.2022.1078394 |
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