Cargando…

Effects of replacing fishmeal with cottonseed protein concentrate on growth performance, blood metabolites, and the intestinal health of juvenile rainbow trout (Oncorhynchus mykiss)

Cottonseed protein concentrate (CPC) is a potential non-food protein source for fishmeal replacement in fish feed. However, a high inclusion level of CPC in diets may have adverse effects on the metabolism and health of carnivorous fish. This study aimed to investigate CPC as a fishmeal alternative...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Yang, Ma, Shuwei, Lv, Weihua, Shi, Honghe, Qiu, Guangwen, Chang, Hongmiao, Lu, Shaoxia, Wang, Di, Wang, Changan, Han, Shicheng, Liu, Hongbai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9811179/
https://www.ncbi.nlm.nih.gov/pubmed/36618404
http://dx.doi.org/10.3389/fimmu.2022.1079677
_version_ 1784863476109279232
author Liu, Yang
Ma, Shuwei
Lv, Weihua
Shi, Honghe
Qiu, Guangwen
Chang, Hongmiao
Lu, Shaoxia
Wang, Di
Wang, Changan
Han, Shicheng
Liu, Hongbai
author_facet Liu, Yang
Ma, Shuwei
Lv, Weihua
Shi, Honghe
Qiu, Guangwen
Chang, Hongmiao
Lu, Shaoxia
Wang, Di
Wang, Changan
Han, Shicheng
Liu, Hongbai
author_sort Liu, Yang
collection PubMed
description Cottonseed protein concentrate (CPC) is a potential non-food protein source for fishmeal replacement in fish feed. However, a high inclusion level of CPC in diets may have adverse effects on the metabolism and health of carnivorous fish. This study aimed to investigate CPC as a fishmeal alternative in the diet of rainbow trout Oncorhynchus mykiss based on growth performance, blood metabolites, and intestinal health. Five isonitrogenous (46% crude protein) and isolipidic (16% crude lipid) diets were formulated: a control diet (30% fishmeal) and four experimental diets with substitution of fishmeal by CPC at 25%, 50%, 75%, and 100%. A total of 600 fish (mean body weight 11.24g) were hand-fed the five formulated diets to apparent satiation for eight weeks. The results showed no adverse effects on growth performance when 75% dietary fishmeal was replaced by CPC. However, reduced growth and feed intake were observed in rainbow trout fed a fishmeal-free diet based on CPC (CPC100%). Changes in serum metabolites were also observed in CPC100% compared with the control group, including an increase in alanine aminotransferase (ALT), a decrease in alkaline phosphatase (ALP), alterations in free amino acids, and reductions in cholesterol metabolism. In addition, the CPC-based diet resulted in reduced intestinal trypsin, decreased villus height and width in the distal intestine, upregulated mRNA expression levels of inflammatory cytokines in the intestine, and impaired gut microbiota with reduced bacterial diversity and decreased abundance of Bacillaceae compared with the control group. The findings suggest that the optimum substitution rate of dietary fishmeal by CPC for rainbow trout should be less than 75%.
format Online
Article
Text
id pubmed-9811179
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-98111792023-01-05 Effects of replacing fishmeal with cottonseed protein concentrate on growth performance, blood metabolites, and the intestinal health of juvenile rainbow trout (Oncorhynchus mykiss) Liu, Yang Ma, Shuwei Lv, Weihua Shi, Honghe Qiu, Guangwen Chang, Hongmiao Lu, Shaoxia Wang, Di Wang, Changan Han, Shicheng Liu, Hongbai Front Immunol Immunology Cottonseed protein concentrate (CPC) is a potential non-food protein source for fishmeal replacement in fish feed. However, a high inclusion level of CPC in diets may have adverse effects on the metabolism and health of carnivorous fish. This study aimed to investigate CPC as a fishmeal alternative in the diet of rainbow trout Oncorhynchus mykiss based on growth performance, blood metabolites, and intestinal health. Five isonitrogenous (46% crude protein) and isolipidic (16% crude lipid) diets were formulated: a control diet (30% fishmeal) and four experimental diets with substitution of fishmeal by CPC at 25%, 50%, 75%, and 100%. A total of 600 fish (mean body weight 11.24g) were hand-fed the five formulated diets to apparent satiation for eight weeks. The results showed no adverse effects on growth performance when 75% dietary fishmeal was replaced by CPC. However, reduced growth and feed intake were observed in rainbow trout fed a fishmeal-free diet based on CPC (CPC100%). Changes in serum metabolites were also observed in CPC100% compared with the control group, including an increase in alanine aminotransferase (ALT), a decrease in alkaline phosphatase (ALP), alterations in free amino acids, and reductions in cholesterol metabolism. In addition, the CPC-based diet resulted in reduced intestinal trypsin, decreased villus height and width in the distal intestine, upregulated mRNA expression levels of inflammatory cytokines in the intestine, and impaired gut microbiota with reduced bacterial diversity and decreased abundance of Bacillaceae compared with the control group. The findings suggest that the optimum substitution rate of dietary fishmeal by CPC for rainbow trout should be less than 75%. Frontiers Media S.A. 2022-12-21 /pmc/articles/PMC9811179/ /pubmed/36618404 http://dx.doi.org/10.3389/fimmu.2022.1079677 Text en Copyright © 2022 Liu, Ma, Lv, Shi, Qiu, Chang, Lu, Wang, Wang, Han and Liu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Liu, Yang
Ma, Shuwei
Lv, Weihua
Shi, Honghe
Qiu, Guangwen
Chang, Hongmiao
Lu, Shaoxia
Wang, Di
Wang, Changan
Han, Shicheng
Liu, Hongbai
Effects of replacing fishmeal with cottonseed protein concentrate on growth performance, blood metabolites, and the intestinal health of juvenile rainbow trout (Oncorhynchus mykiss)
title Effects of replacing fishmeal with cottonseed protein concentrate on growth performance, blood metabolites, and the intestinal health of juvenile rainbow trout (Oncorhynchus mykiss)
title_full Effects of replacing fishmeal with cottonseed protein concentrate on growth performance, blood metabolites, and the intestinal health of juvenile rainbow trout (Oncorhynchus mykiss)
title_fullStr Effects of replacing fishmeal with cottonseed protein concentrate on growth performance, blood metabolites, and the intestinal health of juvenile rainbow trout (Oncorhynchus mykiss)
title_full_unstemmed Effects of replacing fishmeal with cottonseed protein concentrate on growth performance, blood metabolites, and the intestinal health of juvenile rainbow trout (Oncorhynchus mykiss)
title_short Effects of replacing fishmeal with cottonseed protein concentrate on growth performance, blood metabolites, and the intestinal health of juvenile rainbow trout (Oncorhynchus mykiss)
title_sort effects of replacing fishmeal with cottonseed protein concentrate on growth performance, blood metabolites, and the intestinal health of juvenile rainbow trout (oncorhynchus mykiss)
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9811179/
https://www.ncbi.nlm.nih.gov/pubmed/36618404
http://dx.doi.org/10.3389/fimmu.2022.1079677
work_keys_str_mv AT liuyang effectsofreplacingfishmealwithcottonseedproteinconcentrateongrowthperformancebloodmetabolitesandtheintestinalhealthofjuvenilerainbowtroutoncorhynchusmykiss
AT mashuwei effectsofreplacingfishmealwithcottonseedproteinconcentrateongrowthperformancebloodmetabolitesandtheintestinalhealthofjuvenilerainbowtroutoncorhynchusmykiss
AT lvweihua effectsofreplacingfishmealwithcottonseedproteinconcentrateongrowthperformancebloodmetabolitesandtheintestinalhealthofjuvenilerainbowtroutoncorhynchusmykiss
AT shihonghe effectsofreplacingfishmealwithcottonseedproteinconcentrateongrowthperformancebloodmetabolitesandtheintestinalhealthofjuvenilerainbowtroutoncorhynchusmykiss
AT qiuguangwen effectsofreplacingfishmealwithcottonseedproteinconcentrateongrowthperformancebloodmetabolitesandtheintestinalhealthofjuvenilerainbowtroutoncorhynchusmykiss
AT changhongmiao effectsofreplacingfishmealwithcottonseedproteinconcentrateongrowthperformancebloodmetabolitesandtheintestinalhealthofjuvenilerainbowtroutoncorhynchusmykiss
AT lushaoxia effectsofreplacingfishmealwithcottonseedproteinconcentrateongrowthperformancebloodmetabolitesandtheintestinalhealthofjuvenilerainbowtroutoncorhynchusmykiss
AT wangdi effectsofreplacingfishmealwithcottonseedproteinconcentrateongrowthperformancebloodmetabolitesandtheintestinalhealthofjuvenilerainbowtroutoncorhynchusmykiss
AT wangchangan effectsofreplacingfishmealwithcottonseedproteinconcentrateongrowthperformancebloodmetabolitesandtheintestinalhealthofjuvenilerainbowtroutoncorhynchusmykiss
AT hanshicheng effectsofreplacingfishmealwithcottonseedproteinconcentrateongrowthperformancebloodmetabolitesandtheintestinalhealthofjuvenilerainbowtroutoncorhynchusmykiss
AT liuhongbai effectsofreplacingfishmealwithcottonseedproteinconcentrateongrowthperformancebloodmetabolitesandtheintestinalhealthofjuvenilerainbowtroutoncorhynchusmykiss