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Effect of substitution of taurine for methionine and additional taurine supplementation on the performance and antioxidative capacity of laying hens
Taurine (TAU), a sulfur-containing amino acid that synthesized from methionine and cystine, plays vital roles in maintenance of redox balance. The effect of substitution of TAU for methionine was evaluated in vivo and in vitro. The effects of replacing methionine with TAU and additional TAU suppleme...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9811254/ https://www.ncbi.nlm.nih.gov/pubmed/36587450 http://dx.doi.org/10.1016/j.psj.2022.102426 |
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author | Yuan, Xiukang Liu, Yu Chen, Yanling Jiao, Hongchao Zhao, Jingpeng Wang, Xiaojuan Zhou, Yunlei Lin, Hai |
author_facet | Yuan, Xiukang Liu, Yu Chen, Yanling Jiao, Hongchao Zhao, Jingpeng Wang, Xiaojuan Zhou, Yunlei Lin, Hai |
author_sort | Yuan, Xiukang |
collection | PubMed |
description | Taurine (TAU), a sulfur-containing amino acid that synthesized from methionine and cystine, plays vital roles in maintenance of redox balance. The effect of substitution of TAU for methionine was evaluated in vivo and in vitro. The effects of replacing methionine with TAU and additional TAU supplementation on the performance and antioxidant capacity of laying hens were evaluated. The in vitro cultured chicken primary hepatocytes and intestinal epithelial cells were further employed. Two hubdred eighty-eight 40-wk-old Isa brown laying hens were divided into 4 groups and subjected one to the following treatments: fed with basal diet with 0.17% crystallized DL-Met (CON), the control diet and replace 25% (21% total Met, 21TAU) or 50% (42% total Met, 42TAU) of crystallized DL-Met with taurine, the control diet supplemented with 0.1% taurine (0.1% TAU). The laying rate, feed intake, egg weight, and feed efficiency were not influenced (P > 0.05) by TAU replacement or additional TAU supplementation. In the liver, 0.1% TAU decreased SOD but increased GSH-Px activity (P < 0.01). In duodenum, 42TAU decreased SOD activity (P < 0.05) while 0.1% TAU decreased GSH level and SOD activity (P < 0.05). In the hepatocytes, TAU treatment decreased (P < 0.05) the MDA and GSH contents, whereas increased SOD and GSH-Px activities (P < 0.05). Meanwhile, TAU treatment decreased (P < 0.05) the protein expression of Nrf2 while increase Keap1 expression. The mRNA expression of Nrf2, SOD1, SOD2, CAT, and GCLC were increased (P < 0.05) and GSR were decreased (P < 0.05) by 0.1% TAU. In the intestinal epithelial cells, TAU treatment decreased (P < 0.05) SOD activity, increased (P < 0.05) CAT activity, and decreased (P < 0.05) the mRNA and protein expression of Nrf2. In summary, partial substitution methionine for taurine (21–42%) has no influence on egg performance of hens. Taurine enhances the antioxidative capacity in hepatocyte but not in the enterocytes and if taurine could offer an improved effect on antioxidant capacity needs to be verified under oxidative stress-challenged conditions. |
format | Online Article Text |
id | pubmed-9811254 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-98112542023-01-05 Effect of substitution of taurine for methionine and additional taurine supplementation on the performance and antioxidative capacity of laying hens Yuan, Xiukang Liu, Yu Chen, Yanling Jiao, Hongchao Zhao, Jingpeng Wang, Xiaojuan Zhou, Yunlei Lin, Hai Poult Sci METABOLISM AND NUTRITION Taurine (TAU), a sulfur-containing amino acid that synthesized from methionine and cystine, plays vital roles in maintenance of redox balance. The effect of substitution of TAU for methionine was evaluated in vivo and in vitro. The effects of replacing methionine with TAU and additional TAU supplementation on the performance and antioxidant capacity of laying hens were evaluated. The in vitro cultured chicken primary hepatocytes and intestinal epithelial cells were further employed. Two hubdred eighty-eight 40-wk-old Isa brown laying hens were divided into 4 groups and subjected one to the following treatments: fed with basal diet with 0.17% crystallized DL-Met (CON), the control diet and replace 25% (21% total Met, 21TAU) or 50% (42% total Met, 42TAU) of crystallized DL-Met with taurine, the control diet supplemented with 0.1% taurine (0.1% TAU). The laying rate, feed intake, egg weight, and feed efficiency were not influenced (P > 0.05) by TAU replacement or additional TAU supplementation. In the liver, 0.1% TAU decreased SOD but increased GSH-Px activity (P < 0.01). In duodenum, 42TAU decreased SOD activity (P < 0.05) while 0.1% TAU decreased GSH level and SOD activity (P < 0.05). In the hepatocytes, TAU treatment decreased (P < 0.05) the MDA and GSH contents, whereas increased SOD and GSH-Px activities (P < 0.05). Meanwhile, TAU treatment decreased (P < 0.05) the protein expression of Nrf2 while increase Keap1 expression. The mRNA expression of Nrf2, SOD1, SOD2, CAT, and GCLC were increased (P < 0.05) and GSR were decreased (P < 0.05) by 0.1% TAU. In the intestinal epithelial cells, TAU treatment decreased (P < 0.05) SOD activity, increased (P < 0.05) CAT activity, and decreased (P < 0.05) the mRNA and protein expression of Nrf2. In summary, partial substitution methionine for taurine (21–42%) has no influence on egg performance of hens. Taurine enhances the antioxidative capacity in hepatocyte but not in the enterocytes and if taurine could offer an improved effect on antioxidant capacity needs to be verified under oxidative stress-challenged conditions. Elsevier 2022-12-15 /pmc/articles/PMC9811254/ /pubmed/36587450 http://dx.doi.org/10.1016/j.psj.2022.102426 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | METABOLISM AND NUTRITION Yuan, Xiukang Liu, Yu Chen, Yanling Jiao, Hongchao Zhao, Jingpeng Wang, Xiaojuan Zhou, Yunlei Lin, Hai Effect of substitution of taurine for methionine and additional taurine supplementation on the performance and antioxidative capacity of laying hens |
title | Effect of substitution of taurine for methionine and additional taurine supplementation on the performance and antioxidative capacity of laying hens |
title_full | Effect of substitution of taurine for methionine and additional taurine supplementation on the performance and antioxidative capacity of laying hens |
title_fullStr | Effect of substitution of taurine for methionine and additional taurine supplementation on the performance and antioxidative capacity of laying hens |
title_full_unstemmed | Effect of substitution of taurine for methionine and additional taurine supplementation on the performance and antioxidative capacity of laying hens |
title_short | Effect of substitution of taurine for methionine and additional taurine supplementation on the performance and antioxidative capacity of laying hens |
title_sort | effect of substitution of taurine for methionine and additional taurine supplementation on the performance and antioxidative capacity of laying hens |
topic | METABOLISM AND NUTRITION |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9811254/ https://www.ncbi.nlm.nih.gov/pubmed/36587450 http://dx.doi.org/10.1016/j.psj.2022.102426 |
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