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Effect of substitution of taurine for methionine and additional taurine supplementation on the performance and antioxidative capacity of laying hens

Taurine (TAU), a sulfur-containing amino acid that synthesized from methionine and cystine, plays vital roles in maintenance of redox balance. The effect of substitution of TAU for methionine was evaluated in vivo and in vitro. The effects of replacing methionine with TAU and additional TAU suppleme...

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Autores principales: Yuan, Xiukang, Liu, Yu, Chen, Yanling, Jiao, Hongchao, Zhao, Jingpeng, Wang, Xiaojuan, Zhou, Yunlei, Lin, Hai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9811254/
https://www.ncbi.nlm.nih.gov/pubmed/36587450
http://dx.doi.org/10.1016/j.psj.2022.102426
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author Yuan, Xiukang
Liu, Yu
Chen, Yanling
Jiao, Hongchao
Zhao, Jingpeng
Wang, Xiaojuan
Zhou, Yunlei
Lin, Hai
author_facet Yuan, Xiukang
Liu, Yu
Chen, Yanling
Jiao, Hongchao
Zhao, Jingpeng
Wang, Xiaojuan
Zhou, Yunlei
Lin, Hai
author_sort Yuan, Xiukang
collection PubMed
description Taurine (TAU), a sulfur-containing amino acid that synthesized from methionine and cystine, plays vital roles in maintenance of redox balance. The effect of substitution of TAU for methionine was evaluated in vivo and in vitro. The effects of replacing methionine with TAU and additional TAU supplementation on the performance and antioxidant capacity of laying hens were evaluated. The in vitro cultured chicken primary hepatocytes and intestinal epithelial cells were further employed. Two hubdred eighty-eight 40-wk-old Isa brown laying hens were divided into 4 groups and subjected one to the following treatments: fed with basal diet with 0.17% crystallized DL-Met (CON), the control diet and replace 25% (21% total Met, 21TAU) or 50% (42% total Met, 42TAU) of crystallized DL-Met with taurine, the control diet supplemented with 0.1% taurine (0.1% TAU). The laying rate, feed intake, egg weight, and feed efficiency were not influenced (P > 0.05) by TAU replacement or additional TAU supplementation. In the liver, 0.1% TAU decreased SOD but increased GSH-Px activity (P < 0.01). In duodenum, 42TAU decreased SOD activity (P < 0.05) while 0.1% TAU decreased GSH level and SOD activity (P < 0.05). In the hepatocytes, TAU treatment decreased (P < 0.05) the MDA and GSH contents, whereas increased SOD and GSH-Px activities (P < 0.05). Meanwhile, TAU treatment decreased (P < 0.05) the protein expression of Nrf2 while increase Keap1 expression. The mRNA expression of Nrf2, SOD1, SOD2, CAT, and GCLC were increased (P < 0.05) and GSR were decreased (P < 0.05) by 0.1% TAU. In the intestinal epithelial cells, TAU treatment decreased (P < 0.05) SOD activity, increased (P < 0.05) CAT activity, and decreased (P < 0.05) the mRNA and protein expression of Nrf2. In summary, partial substitution methionine for taurine (21–42%) has no influence on egg performance of hens. Taurine enhances the antioxidative capacity in hepatocyte but not in the enterocytes and if taurine could offer an improved effect on antioxidant capacity needs to be verified under oxidative stress-challenged conditions.
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spelling pubmed-98112542023-01-05 Effect of substitution of taurine for methionine and additional taurine supplementation on the performance and antioxidative capacity of laying hens Yuan, Xiukang Liu, Yu Chen, Yanling Jiao, Hongchao Zhao, Jingpeng Wang, Xiaojuan Zhou, Yunlei Lin, Hai Poult Sci METABOLISM AND NUTRITION Taurine (TAU), a sulfur-containing amino acid that synthesized from methionine and cystine, plays vital roles in maintenance of redox balance. The effect of substitution of TAU for methionine was evaluated in vivo and in vitro. The effects of replacing methionine with TAU and additional TAU supplementation on the performance and antioxidant capacity of laying hens were evaluated. The in vitro cultured chicken primary hepatocytes and intestinal epithelial cells were further employed. Two hubdred eighty-eight 40-wk-old Isa brown laying hens were divided into 4 groups and subjected one to the following treatments: fed with basal diet with 0.17% crystallized DL-Met (CON), the control diet and replace 25% (21% total Met, 21TAU) or 50% (42% total Met, 42TAU) of crystallized DL-Met with taurine, the control diet supplemented with 0.1% taurine (0.1% TAU). The laying rate, feed intake, egg weight, and feed efficiency were not influenced (P > 0.05) by TAU replacement or additional TAU supplementation. In the liver, 0.1% TAU decreased SOD but increased GSH-Px activity (P < 0.01). In duodenum, 42TAU decreased SOD activity (P < 0.05) while 0.1% TAU decreased GSH level and SOD activity (P < 0.05). In the hepatocytes, TAU treatment decreased (P < 0.05) the MDA and GSH contents, whereas increased SOD and GSH-Px activities (P < 0.05). Meanwhile, TAU treatment decreased (P < 0.05) the protein expression of Nrf2 while increase Keap1 expression. The mRNA expression of Nrf2, SOD1, SOD2, CAT, and GCLC were increased (P < 0.05) and GSR were decreased (P < 0.05) by 0.1% TAU. In the intestinal epithelial cells, TAU treatment decreased (P < 0.05) SOD activity, increased (P < 0.05) CAT activity, and decreased (P < 0.05) the mRNA and protein expression of Nrf2. In summary, partial substitution methionine for taurine (21–42%) has no influence on egg performance of hens. Taurine enhances the antioxidative capacity in hepatocyte but not in the enterocytes and if taurine could offer an improved effect on antioxidant capacity needs to be verified under oxidative stress-challenged conditions. Elsevier 2022-12-15 /pmc/articles/PMC9811254/ /pubmed/36587450 http://dx.doi.org/10.1016/j.psj.2022.102426 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle METABOLISM AND NUTRITION
Yuan, Xiukang
Liu, Yu
Chen, Yanling
Jiao, Hongchao
Zhao, Jingpeng
Wang, Xiaojuan
Zhou, Yunlei
Lin, Hai
Effect of substitution of taurine for methionine and additional taurine supplementation on the performance and antioxidative capacity of laying hens
title Effect of substitution of taurine for methionine and additional taurine supplementation on the performance and antioxidative capacity of laying hens
title_full Effect of substitution of taurine for methionine and additional taurine supplementation on the performance and antioxidative capacity of laying hens
title_fullStr Effect of substitution of taurine for methionine and additional taurine supplementation on the performance and antioxidative capacity of laying hens
title_full_unstemmed Effect of substitution of taurine for methionine and additional taurine supplementation on the performance and antioxidative capacity of laying hens
title_short Effect of substitution of taurine for methionine and additional taurine supplementation on the performance and antioxidative capacity of laying hens
title_sort effect of substitution of taurine for methionine and additional taurine supplementation on the performance and antioxidative capacity of laying hens
topic METABOLISM AND NUTRITION
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9811254/
https://www.ncbi.nlm.nih.gov/pubmed/36587450
http://dx.doi.org/10.1016/j.psj.2022.102426
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