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Longitudinal Evaluation of Biomarkers in Wound Fluids from Venous Leg Ulcers and Split-thickness Skin Graft Donor Site Wounds Treated with a Protease-modulating Wound Dressing
Venous leg ulcers represent a clinical challenge and impair the quality of life of patients. This study examines impaired wound healing in venous leg ulcers at the molecular level. Protein expression patterns for biomarkers were analysed in venous leg ulcer wound fluids from 57 patients treated with...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Society for Publication of Acta Dermato-Venereologica
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9811302/ https://www.ncbi.nlm.nih.gov/pubmed/36250733 http://dx.doi.org/10.2340/actadv.v102.325 |
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author | MIKOSIŃSKI, Jacek KALOGEROPOULOS, Konstantinos BUNDGAARD, Louise LARSEN, Cathrine Agnete SAVICKAS, Simonas HAACK, Aleksander Moldt PAŃCZAK, Konrad RYBOŁOWICZ, Katarzyna GRZELA, Tomasz OLSZEWSKI, Michał CISZEWSKI, Piotr SITEK-ZIÓŁKOWSKA, Karina TWARDOWSKA-SAUCHA, Krystyna KARCZEWSKI, Marek RABCZENKO, Daniel SEGIET, Agnieszka BUCZAK-KULA, Patrycja SCHOOF, Erwin M. EMING, Sabine A. SMOLA, Hans AUF DEM KELLER, Ulrich |
author_facet | MIKOSIŃSKI, Jacek KALOGEROPOULOS, Konstantinos BUNDGAARD, Louise LARSEN, Cathrine Agnete SAVICKAS, Simonas HAACK, Aleksander Moldt PAŃCZAK, Konrad RYBOŁOWICZ, Katarzyna GRZELA, Tomasz OLSZEWSKI, Michał CISZEWSKI, Piotr SITEK-ZIÓŁKOWSKA, Karina TWARDOWSKA-SAUCHA, Krystyna KARCZEWSKI, Marek RABCZENKO, Daniel SEGIET, Agnieszka BUCZAK-KULA, Patrycja SCHOOF, Erwin M. EMING, Sabine A. SMOLA, Hans AUF DEM KELLER, Ulrich |
author_sort | MIKOSIŃSKI, Jacek |
collection | PubMed |
description | Venous leg ulcers represent a clinical challenge and impair the quality of life of patients. This study examines impaired wound healing in venous leg ulcers at the molecular level. Protein expression patterns for biomarkers were analysed in venous leg ulcer wound fluids from 57 patients treated with a protease-modulating polyacrylate wound dressing for 12 weeks, and compared with exudates from 10 acute split-thickness wounds. Wound healing improved in the venous leg ulcer wounds: 61.4% of the 57 patients with venous leg ulcer achieved a relative wound area reduction of ≥ 40%, and 50.9% of the total 57 patients achieved a relative wound area reduction of ≥ 60%. Within the first 14 days, abundances of S100A8, S100A9, neutrophil elastase, matrix metalloproteinase-2, and fibronectin in venous leg ulcer exudates decreased significantly and remained stable, yet higher than in acute wounds. Interleukin-1β, tumour necrosis factor alpha, and matrix metalloproteinase-9 abundance ranges were similar in venous leg ulcers and acute wound fluids. Collagen (I) α1 abundance was higher in venous leg ulcer wound fluids and was not significantly regulated. Overall, significant biomarker changes occurred in the first 14 days before a clinically robust healing response in the venous leg ulcer cohort. |
format | Online Article Text |
id | pubmed-9811302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Society for Publication of Acta Dermato-Venereologica |
record_format | MEDLINE/PubMed |
spelling | pubmed-98113022023-02-08 Longitudinal Evaluation of Biomarkers in Wound Fluids from Venous Leg Ulcers and Split-thickness Skin Graft Donor Site Wounds Treated with a Protease-modulating Wound Dressing MIKOSIŃSKI, Jacek KALOGEROPOULOS, Konstantinos BUNDGAARD, Louise LARSEN, Cathrine Agnete SAVICKAS, Simonas HAACK, Aleksander Moldt PAŃCZAK, Konrad RYBOŁOWICZ, Katarzyna GRZELA, Tomasz OLSZEWSKI, Michał CISZEWSKI, Piotr SITEK-ZIÓŁKOWSKA, Karina TWARDOWSKA-SAUCHA, Krystyna KARCZEWSKI, Marek RABCZENKO, Daniel SEGIET, Agnieszka BUCZAK-KULA, Patrycja SCHOOF, Erwin M. EMING, Sabine A. SMOLA, Hans AUF DEM KELLER, Ulrich Acta Derm Venereol Original Article Venous leg ulcers represent a clinical challenge and impair the quality of life of patients. This study examines impaired wound healing in venous leg ulcers at the molecular level. Protein expression patterns for biomarkers were analysed in venous leg ulcer wound fluids from 57 patients treated with a protease-modulating polyacrylate wound dressing for 12 weeks, and compared with exudates from 10 acute split-thickness wounds. Wound healing improved in the venous leg ulcer wounds: 61.4% of the 57 patients with venous leg ulcer achieved a relative wound area reduction of ≥ 40%, and 50.9% of the total 57 patients achieved a relative wound area reduction of ≥ 60%. Within the first 14 days, abundances of S100A8, S100A9, neutrophil elastase, matrix metalloproteinase-2, and fibronectin in venous leg ulcer exudates decreased significantly and remained stable, yet higher than in acute wounds. Interleukin-1β, tumour necrosis factor alpha, and matrix metalloproteinase-9 abundance ranges were similar in venous leg ulcers and acute wound fluids. Collagen (I) α1 abundance was higher in venous leg ulcer wound fluids and was not significantly regulated. Overall, significant biomarker changes occurred in the first 14 days before a clinically robust healing response in the venous leg ulcer cohort. Society for Publication of Acta Dermato-Venereologica 2022-12-13 /pmc/articles/PMC9811302/ /pubmed/36250733 http://dx.doi.org/10.2340/actadv.v102.325 Text en © 2022 Acta Dermato-Venereologica https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the CC BY-NC license |
spellingShingle | Original Article MIKOSIŃSKI, Jacek KALOGEROPOULOS, Konstantinos BUNDGAARD, Louise LARSEN, Cathrine Agnete SAVICKAS, Simonas HAACK, Aleksander Moldt PAŃCZAK, Konrad RYBOŁOWICZ, Katarzyna GRZELA, Tomasz OLSZEWSKI, Michał CISZEWSKI, Piotr SITEK-ZIÓŁKOWSKA, Karina TWARDOWSKA-SAUCHA, Krystyna KARCZEWSKI, Marek RABCZENKO, Daniel SEGIET, Agnieszka BUCZAK-KULA, Patrycja SCHOOF, Erwin M. EMING, Sabine A. SMOLA, Hans AUF DEM KELLER, Ulrich Longitudinal Evaluation of Biomarkers in Wound Fluids from Venous Leg Ulcers and Split-thickness Skin Graft Donor Site Wounds Treated with a Protease-modulating Wound Dressing |
title | Longitudinal Evaluation of Biomarkers in Wound Fluids from Venous Leg Ulcers and Split-thickness Skin Graft Donor Site Wounds Treated with a Protease-modulating Wound Dressing |
title_full | Longitudinal Evaluation of Biomarkers in Wound Fluids from Venous Leg Ulcers and Split-thickness Skin Graft Donor Site Wounds Treated with a Protease-modulating Wound Dressing |
title_fullStr | Longitudinal Evaluation of Biomarkers in Wound Fluids from Venous Leg Ulcers and Split-thickness Skin Graft Donor Site Wounds Treated with a Protease-modulating Wound Dressing |
title_full_unstemmed | Longitudinal Evaluation of Biomarkers in Wound Fluids from Venous Leg Ulcers and Split-thickness Skin Graft Donor Site Wounds Treated with a Protease-modulating Wound Dressing |
title_short | Longitudinal Evaluation of Biomarkers in Wound Fluids from Venous Leg Ulcers and Split-thickness Skin Graft Donor Site Wounds Treated with a Protease-modulating Wound Dressing |
title_sort | longitudinal evaluation of biomarkers in wound fluids from venous leg ulcers and split-thickness skin graft donor site wounds treated with a protease-modulating wound dressing |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9811302/ https://www.ncbi.nlm.nih.gov/pubmed/36250733 http://dx.doi.org/10.2340/actadv.v102.325 |
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