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The durability of resin–dentine bonds are enhanced by epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica
Biomimetic nanohydroxyapatite (nHAp) has long been used as a biocompatible material for bone repair, bone regeneration, and bone reconstruction due to its low toxicity to local or systemic tissues. Various cross‐linkers have been employed to maintain the structure of collagen; these include epigallo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9811609/ https://www.ncbi.nlm.nih.gov/pubmed/36350226 http://dx.doi.org/10.1002/2211-5463.13521 |
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author | Zhang, Taiyang Deng, Wei Zhang, Ying Liu, Ming Ling, Yongchang Sun, Qiurong |
author_facet | Zhang, Taiyang Deng, Wei Zhang, Ying Liu, Ming Ling, Yongchang Sun, Qiurong |
author_sort | Zhang, Taiyang |
collection | PubMed |
description | Biomimetic nanohydroxyapatite (nHAp) has long been used as a biocompatible material for bone repair, bone regeneration, and bone reconstruction due to its low toxicity to local or systemic tissues. Various cross‐linkers have been employed to maintain the structure of collagen; these include epigallocatechin‐3‐gallate (EGCG), which can fortify the mechanical properties of collagen and withstand the degradation of collagenase. We hypothesized that EGCG combined with nHAp may promote resin–dentin bonding durability. Here, we examined the effect of epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica (EGCG@nHAp@MSN) on thermal stability and remineralization capability of dentin collagen. Dentin slices (2 × 2 × 1 mm(3)) were obtained and completely demineralized in a 10% phosphoric acid water solution. The resulting dentin collagen matrix was incubated with deionized water, EGCG, nHAp@MSN, and EGCG@nHAp@MSN. The collagen thermal degradation temperature was assessed utilizing differential scanning calorimetry analysis, which indicated that EGCG, nHAp@MSN, and EGCG@nHAp@MSN reinforced collagen's capability to resist thermal degradation. EGCG@nHAp@MSN resulted in the highest increase in denaturation temperature. Thermogravimetric analysis showed that both nHAp@MSN and EGCG@nHAp@MSN achieved a higher residual mass than the EGCG and control groups. Fourier transform infrared spectroscopy was performed to examine the interaction between EGCG@nHAp@MSN and dentin collagen. The EGCG@nHAp@MSN sample exhibited stronger dentin microhardness and uppermost bond strength after thermocycling. EGCG significantly enhanced collagen's capability to resist thermal degradation. In summary, EGCG and nHAp@MSN may work together to assist the exposed collagen to improve resistance to thermal cycling and promote remineralization while also strengthening the durability of resin–dentin bonds. |
format | Online Article Text |
id | pubmed-9811609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98116092023-01-05 The durability of resin–dentine bonds are enhanced by epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica Zhang, Taiyang Deng, Wei Zhang, Ying Liu, Ming Ling, Yongchang Sun, Qiurong FEBS Open Bio Research Articles Biomimetic nanohydroxyapatite (nHAp) has long been used as a biocompatible material for bone repair, bone regeneration, and bone reconstruction due to its low toxicity to local or systemic tissues. Various cross‐linkers have been employed to maintain the structure of collagen; these include epigallocatechin‐3‐gallate (EGCG), which can fortify the mechanical properties of collagen and withstand the degradation of collagenase. We hypothesized that EGCG combined with nHAp may promote resin–dentin bonding durability. Here, we examined the effect of epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica (EGCG@nHAp@MSN) on thermal stability and remineralization capability of dentin collagen. Dentin slices (2 × 2 × 1 mm(3)) were obtained and completely demineralized in a 10% phosphoric acid water solution. The resulting dentin collagen matrix was incubated with deionized water, EGCG, nHAp@MSN, and EGCG@nHAp@MSN. The collagen thermal degradation temperature was assessed utilizing differential scanning calorimetry analysis, which indicated that EGCG, nHAp@MSN, and EGCG@nHAp@MSN reinforced collagen's capability to resist thermal degradation. EGCG@nHAp@MSN resulted in the highest increase in denaturation temperature. Thermogravimetric analysis showed that both nHAp@MSN and EGCG@nHAp@MSN achieved a higher residual mass than the EGCG and control groups. Fourier transform infrared spectroscopy was performed to examine the interaction between EGCG@nHAp@MSN and dentin collagen. The EGCG@nHAp@MSN sample exhibited stronger dentin microhardness and uppermost bond strength after thermocycling. EGCG significantly enhanced collagen's capability to resist thermal degradation. In summary, EGCG and nHAp@MSN may work together to assist the exposed collagen to improve resistance to thermal cycling and promote remineralization while also strengthening the durability of resin–dentin bonds. John Wiley and Sons Inc. 2022-11-22 /pmc/articles/PMC9811609/ /pubmed/36350226 http://dx.doi.org/10.1002/2211-5463.13521 Text en © 2022 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Zhang, Taiyang Deng, Wei Zhang, Ying Liu, Ming Ling, Yongchang Sun, Qiurong The durability of resin–dentine bonds are enhanced by epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica |
title | The durability of resin–dentine bonds are enhanced by epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica |
title_full | The durability of resin–dentine bonds are enhanced by epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica |
title_fullStr | The durability of resin–dentine bonds are enhanced by epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica |
title_full_unstemmed | The durability of resin–dentine bonds are enhanced by epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica |
title_short | The durability of resin–dentine bonds are enhanced by epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica |
title_sort | durability of resin–dentine bonds are enhanced by epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9811609/ https://www.ncbi.nlm.nih.gov/pubmed/36350226 http://dx.doi.org/10.1002/2211-5463.13521 |
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