Cargando…

Fully automated multi-grid cryoEM screening using Smart Leginon

Single-particle cryo-electron microscopy (cryoEM) is a swiftly growing method for understanding protein structure. With increasing demand for high-throughput, high-resolution cryoEM services comes greater demand for rapid and automated cryoEM grid and sample screening. During screening, optimal grid...

Descripción completa

Detalles Bibliográficos
Autores principales:	Cheng, Anchi, Kim, Paul T., Kuang, Huihui, Mendez, Joshua H., Chua, Eugene Y. D., Maruthi, Kashyap, Wei, Hui, Sawh, Anjelique, Aragon, Mahira F., Serbynovskyi, Viacheslav, Neselu, Kasahun, Eng, Edward T., Potter, Clinton S., Carragher, Bridget, Bepler, Tristan, Noble, Alex J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	International Union of Crystallography 2023
Materias:	Research Papers
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9812217/ https://www.ncbi.nlm.nih.gov/pubmed/36598504 http://dx.doi.org/10.1107/S2052252522010624

Ejemplares similares

Routine single particle CryoEM sample and grid characterization by tomography
por: Noble, Alex J, et al.
Publicado: (2018)

Time-resolved cryoEM using Spotiton
por: Dandey, Venkata P., et al.
Publicado: (2020)

Topaz-Denoise: general deep denoising models for cryoEM and cryoET
por: Bepler, Tristan, et al.
Publicado: (2020)

Reducing effects of particle adsorption to the air-water interface in cryoEM
por: Noble, Alex J., et al.
Publicado: (2018)

Measuring the effects of ice thickness on resolution in single particle cryo-EM
por: Neselu, Kasahun, et al.
Publicado: (2023)

Learning to automate cryo-electron microscopy data collection with Ptolemy
por: Kim, Paul T., et al.
Publicado: (2023)

Structure of the Insulin Receptor-Insulin Complex by Single Particle CryoEM analysis
por: Scapin, Giovanna, et al.
Publicado: (2018)

Epoxidized graphene grid for highly efficient high-resolution cryoEM structural analysis
por: Fujita, Junso, et al.
Publicado: (2023)

Need for Speed: Examining Protein Behavior during CryoEM Grid Preparation at Different Timescales
por: Klebl, David P., et al.
Publicado: (2020)

AlphaFold2 and CryoEM: Revisiting CryoEM modeling in near-atomic resolution density maps
por: Hryc, Corey F., et al.
Publicado: (2022)

2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy
por: Campbell, Melody G, et al.
Publicado: (2015)

Studying bacterial chemosensory array with CryoEM
por: Qin, Zhuan, et al.
Publicado: (2021)

Forty years in cryoEM of membrane proteins
por: Kühlbrandt, Werner
Publicado: (2022)

Benchmarking cryo-EM Single Particle Analysis Workflow
por: Kim, Laura Y., et al.
Publicado: (2018)

Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme
por: Zhang, Zhening, et al.
Publicado: (2018)

CryoEM at IUCrJ: a new era
por: Subramaniam, Sriram, et al.
Publicado: (2016)

CryoEM structure of the spliceosome immediately after branching
por: Galej, Wojciech P., et al.
Publicado: (2016)

CryoEM structure of the Nipah virus nucleocapsid assembly
por: Ker, De-Sheng, et al.
Publicado: (2021)

CryoEM Reveals the Complexity and Diversity of ATP Synthases
por: Courbon, Gautier M., et al.
Publicado: (2022)

Nucleosomes and their complexes in the cryoEM era: Trends and limitations
por: Armeev, Grigoriy A., et al.
Publicado: (2022)

EMDataBank.org: unified data resource for CryoEM
por: Lawson, Catherine L., et al.
Publicado: (2011)

CryoEM Visualization of an Adenovirus Capsid-Incorporated HIV Antigen
por: Flatt, Justin W., et al.
Publicado: (2012)

CryoEM structure of the tegumented capsid of Epstein-Barr virus
por: Li, Zhihai, et al.
Publicado: (2020)

Measurement of Atom Resolvability in CryoEM Maps with Q-scores
por: Pintilie, Grigore, et al.
Publicado: (2020)

CryoEM at 100 keV: a demonstration and prospects
por: Naydenova, K., et al.
Publicado: (2019)

From structure to sequence: Antibody discovery using cryoEM
por: Antanasijevic, Aleksandar, et al.
Publicado: (2022)

Development of CryoVR, a virtual reality training system for hands-on cryoEM operations
por: Dong, Jiahui, et al.
Publicado: (2022)

Using cryoEM and cryoET to visualize membrane penetration of a non-enveloped virus
por: Xia, Xian, et al.
Publicado: (2022)

A Pseudo-Atomic Model of the COPII Cage Obtained from CryoEM and Mass Spectrometry Analyses
por: Noble, Alex J., et al.
Publicado: (2012)

Estimating loop length from CryoEM images at medium resolutions
por: McKnight, Andrew, et al.
Publicado: (2013)

Microscopic charge fluctuations cause minimal contrast loss in cryoEM
por: Russo, Christopher J., et al.
Publicado: (2018)

Atoms out of Blobs: CryoEM Takes the Nobel Prize in Chemistry
por: Bertozzi, Carolyn
Publicado: (2017)

CryoEM structures of the human INO80 chromatin remodelling complex
por: Aramayo, Ricardo J., et al.
Publicado: (2017)

Advances in cryoEM and its impact on β-pore forming proteins
por: Boyd, Courtney M, et al.
Publicado: (2018)

Interpretation of medium resolution cryoEM maps of multi-protein complexes
por: Casañal, Ana, et al.
Publicado: (2019)

Interaction of human erythrocyte catalase with air–water interface in cryoEM
por: Chen, Shaoxia, et al.
Publicado: (2022)

Principal component analysis is limited to low-resolution analysis in cryoEM
por: Sorzano, Carlos Oscar S., et al.
Publicado: (2021)

The allosteric control mechanism of bacterial glycogen biosynthesis disclosed by cryoEM
por: Cifuente, Javier O., et al.
Publicado: (2020)

Emerging Themes in CryoEM—Single Particle Analysis Image Processing
por: Vilas, Jose Luis, et al.
Publicado: (2022)

The His-tag as a decoy modulating preferred orientation in cryoEM
por: Bromberg, Raquel, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_si8he5ngo2stdgrj8fpqbgig3o