A large-scale genetic screen identifies genes essential for motility in Agrobacterium fabrum

The genetic and molecular basis of flagellar motility has been investigated for several decades, with innovative research strategies propelling advances at a steady pace. Furthermore, as the phenomenon is examined in diverse bacteria, new taxon-specific regulatory and structural features are being e...

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Autores principales: Calvopina-Chavez, Diana G., Howarth, Robyn E., Memmott, Audrey K., Pech Gonzalez, Oscar H., Hafen, Caleb B., Jensen, Kyson T., Benedict, Alex B., Altman, Jessica D., Burnside, Brittany S., Childs, Justin S., Dallon, Samuel W., DeMarco, Alexa C., Flindt, Kirsten C., Grover, Sarah A., Heninger, Elizabeth, Iverson, Christina S., Johnson, Abigail K., Lopez, Jack B., Meinzer, McKay A., Moulder, Brook A., Moulton, Rebecca I., Russell, Hyrum S., Scott, Tiana M., Shiobara, Yuka, Taylor, Mason D., Tippets, Kathryn E., Vainerere, Kayla M., Von Wallwitz, Isabella C., Wagley, Madison, Wiley, Megumi S., Young, Naomi J., Griffitts, Joel S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9812332/
https://www.ncbi.nlm.nih.gov/pubmed/36598925
http://dx.doi.org/10.1371/journal.pone.0279936
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author Calvopina-Chavez, Diana G.
Howarth, Robyn E.
Memmott, Audrey K.
Pech Gonzalez, Oscar H.
Hafen, Caleb B.
Jensen, Kyson T.
Benedict, Alex B.
Altman, Jessica D.
Burnside, Brittany S.
Childs, Justin S.
Dallon, Samuel W.
DeMarco, Alexa C.
Flindt, Kirsten C.
Grover, Sarah A.
Heninger, Elizabeth
Iverson, Christina S.
Johnson, Abigail K.
Lopez, Jack B.
Meinzer, McKay A.
Moulder, Brook A.
Moulton, Rebecca I.
Russell, Hyrum S.
Scott, Tiana M.
Shiobara, Yuka
Taylor, Mason D.
Tippets, Kathryn E.
Vainerere, Kayla M.
Von Wallwitz, Isabella C.
Wagley, Madison
Wiley, Megumi S.
Young, Naomi J.
Griffitts, Joel S.
author_facet Calvopina-Chavez, Diana G.
Howarth, Robyn E.
Memmott, Audrey K.
Pech Gonzalez, Oscar H.
Hafen, Caleb B.
Jensen, Kyson T.
Benedict, Alex B.
Altman, Jessica D.
Burnside, Brittany S.
Childs, Justin S.
Dallon, Samuel W.
DeMarco, Alexa C.
Flindt, Kirsten C.
Grover, Sarah A.
Heninger, Elizabeth
Iverson, Christina S.
Johnson, Abigail K.
Lopez, Jack B.
Meinzer, McKay A.
Moulder, Brook A.
Moulton, Rebecca I.
Russell, Hyrum S.
Scott, Tiana M.
Shiobara, Yuka
Taylor, Mason D.
Tippets, Kathryn E.
Vainerere, Kayla M.
Von Wallwitz, Isabella C.
Wagley, Madison
Wiley, Megumi S.
Young, Naomi J.
Griffitts, Joel S.
author_sort Calvopina-Chavez, Diana G.
collection PubMed
description The genetic and molecular basis of flagellar motility has been investigated for several decades, with innovative research strategies propelling advances at a steady pace. Furthermore, as the phenomenon is examined in diverse bacteria, new taxon-specific regulatory and structural features are being elucidated. Motility is also a straightforward bacterial phenotype that can allow undergraduate researchers to explore the palette of molecular genetic tools available to microbiologists. This study, driven primarily by undergraduate researchers, evaluated hundreds of flagellar motility mutants in the Gram-negative plant-associated bacterium Agrobacterium fabrum. The nearly saturating screen implicates a total of 37 genes in flagellar biosynthesis, including genes of previously unknown function.
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spelling pubmed-98123322023-01-05 A large-scale genetic screen identifies genes essential for motility in Agrobacterium fabrum Calvopina-Chavez, Diana G. Howarth, Robyn E. Memmott, Audrey K. Pech Gonzalez, Oscar H. Hafen, Caleb B. Jensen, Kyson T. Benedict, Alex B. Altman, Jessica D. Burnside, Brittany S. Childs, Justin S. Dallon, Samuel W. DeMarco, Alexa C. Flindt, Kirsten C. Grover, Sarah A. Heninger, Elizabeth Iverson, Christina S. Johnson, Abigail K. Lopez, Jack B. Meinzer, McKay A. Moulder, Brook A. Moulton, Rebecca I. Russell, Hyrum S. Scott, Tiana M. Shiobara, Yuka Taylor, Mason D. Tippets, Kathryn E. Vainerere, Kayla M. Von Wallwitz, Isabella C. Wagley, Madison Wiley, Megumi S. Young, Naomi J. Griffitts, Joel S. PLoS One Research Article The genetic and molecular basis of flagellar motility has been investigated for several decades, with innovative research strategies propelling advances at a steady pace. Furthermore, as the phenomenon is examined in diverse bacteria, new taxon-specific regulatory and structural features are being elucidated. Motility is also a straightforward bacterial phenotype that can allow undergraduate researchers to explore the palette of molecular genetic tools available to microbiologists. This study, driven primarily by undergraduate researchers, evaluated hundreds of flagellar motility mutants in the Gram-negative plant-associated bacterium Agrobacterium fabrum. The nearly saturating screen implicates a total of 37 genes in flagellar biosynthesis, including genes of previously unknown function. Public Library of Science 2023-01-04 /pmc/articles/PMC9812332/ /pubmed/36598925 http://dx.doi.org/10.1371/journal.pone.0279936 Text en © 2023 Calvopina-Chavez et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Calvopina-Chavez, Diana G.
Howarth, Robyn E.
Memmott, Audrey K.
Pech Gonzalez, Oscar H.
Hafen, Caleb B.
Jensen, Kyson T.
Benedict, Alex B.
Altman, Jessica D.
Burnside, Brittany S.
Childs, Justin S.
Dallon, Samuel W.
DeMarco, Alexa C.
Flindt, Kirsten C.
Grover, Sarah A.
Heninger, Elizabeth
Iverson, Christina S.
Johnson, Abigail K.
Lopez, Jack B.
Meinzer, McKay A.
Moulder, Brook A.
Moulton, Rebecca I.
Russell, Hyrum S.
Scott, Tiana M.
Shiobara, Yuka
Taylor, Mason D.
Tippets, Kathryn E.
Vainerere, Kayla M.
Von Wallwitz, Isabella C.
Wagley, Madison
Wiley, Megumi S.
Young, Naomi J.
Griffitts, Joel S.
A large-scale genetic screen identifies genes essential for motility in Agrobacterium fabrum
title A large-scale genetic screen identifies genes essential for motility in Agrobacterium fabrum
title_full A large-scale genetic screen identifies genes essential for motility in Agrobacterium fabrum
title_fullStr A large-scale genetic screen identifies genes essential for motility in Agrobacterium fabrum
title_full_unstemmed A large-scale genetic screen identifies genes essential for motility in Agrobacterium fabrum
title_short A large-scale genetic screen identifies genes essential for motility in Agrobacterium fabrum
title_sort large-scale genetic screen identifies genes essential for motility in agrobacterium fabrum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9812332/
https://www.ncbi.nlm.nih.gov/pubmed/36598925
http://dx.doi.org/10.1371/journal.pone.0279936
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