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Germline/soma distinction in Drosophila embryos requires regulators of zygotic genome activation
In Drosophila melanogaster embryos, somatic versus germline identity is the first cell fate decision. Zygotic genome activation (ZGA) orchestrates regionalized gene expression, imparting specific identity on somatic cells. ZGA begins with a minor wave that commences at nuclear cycle (NC)8 under the...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9812407/ https://www.ncbi.nlm.nih.gov/pubmed/36598809 http://dx.doi.org/10.7554/eLife.78188 |
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author | Colonnetta, Megan M Schedl, Paul Deshpande, Girish |
author_facet | Colonnetta, Megan M Schedl, Paul Deshpande, Girish |
author_sort | Colonnetta, Megan M |
collection | PubMed |
description | In Drosophila melanogaster embryos, somatic versus germline identity is the first cell fate decision. Zygotic genome activation (ZGA) orchestrates regionalized gene expression, imparting specific identity on somatic cells. ZGA begins with a minor wave that commences at nuclear cycle (NC)8 under the guidance of chromatin accessibility factors (Zelda, CLAMP, GAF), followed by the major wave during NC14. By contrast, primordial germ cell (PGC) specification requires maternally deposited and posteriorly anchored germline determinants. This is accomplished by a centrosome coordinated release and sequestration of germ plasm during the precocious cellularization of PGCs in NC10. Here, we report a novel requirement for Zelda and CLAMP during the establishment of the germline/soma distinction. When their activity is compromised, PGC determinants are not properly sequestered, and specification is disrupted. Conversely, the spreading of PGC determinants from the posterior pole adversely influences transcription in the neighboring somatic nuclei. These reciprocal aberrations can be correlated with defects in centrosome duplication/separation that are known to induce inappropriate transmission of the germ plasm. Interestingly, consistent with the ability of bone morphogenetic protein (BMP) signaling to influence specification of embryonic PGCs, reduction in the transcript levels of a BMP family ligand, decapentaplegic (dpp), is exacerbated at the posterior pole. |
format | Online Article Text |
id | pubmed-9812407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-98124072023-01-05 Germline/soma distinction in Drosophila embryos requires regulators of zygotic genome activation Colonnetta, Megan M Schedl, Paul Deshpande, Girish eLife Developmental Biology In Drosophila melanogaster embryos, somatic versus germline identity is the first cell fate decision. Zygotic genome activation (ZGA) orchestrates regionalized gene expression, imparting specific identity on somatic cells. ZGA begins with a minor wave that commences at nuclear cycle (NC)8 under the guidance of chromatin accessibility factors (Zelda, CLAMP, GAF), followed by the major wave during NC14. By contrast, primordial germ cell (PGC) specification requires maternally deposited and posteriorly anchored germline determinants. This is accomplished by a centrosome coordinated release and sequestration of germ plasm during the precocious cellularization of PGCs in NC10. Here, we report a novel requirement for Zelda and CLAMP during the establishment of the germline/soma distinction. When their activity is compromised, PGC determinants are not properly sequestered, and specification is disrupted. Conversely, the spreading of PGC determinants from the posterior pole adversely influences transcription in the neighboring somatic nuclei. These reciprocal aberrations can be correlated with defects in centrosome duplication/separation that are known to induce inappropriate transmission of the germ plasm. Interestingly, consistent with the ability of bone morphogenetic protein (BMP) signaling to influence specification of embryonic PGCs, reduction in the transcript levels of a BMP family ligand, decapentaplegic (dpp), is exacerbated at the posterior pole. eLife Sciences Publications, Ltd 2023-01-04 /pmc/articles/PMC9812407/ /pubmed/36598809 http://dx.doi.org/10.7554/eLife.78188 Text en © 2023, Colonnetta et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology Colonnetta, Megan M Schedl, Paul Deshpande, Girish Germline/soma distinction in Drosophila embryos requires regulators of zygotic genome activation |
title | Germline/soma distinction in Drosophila embryos requires regulators of zygotic genome activation |
title_full | Germline/soma distinction in Drosophila embryos requires regulators of zygotic genome activation |
title_fullStr | Germline/soma distinction in Drosophila embryos requires regulators of zygotic genome activation |
title_full_unstemmed | Germline/soma distinction in Drosophila embryos requires regulators of zygotic genome activation |
title_short | Germline/soma distinction in Drosophila embryos requires regulators of zygotic genome activation |
title_sort | germline/soma distinction in drosophila embryos requires regulators of zygotic genome activation |
topic | Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9812407/ https://www.ncbi.nlm.nih.gov/pubmed/36598809 http://dx.doi.org/10.7554/eLife.78188 |
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