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Characterization, immunostimulatory and antitumor activities of a β-galactoglucofurannan from cultivated Sanghuangporus vaninii under forest

A biomacromolecule, named as β-galactoglucofurannan (SVPS2), was isolated from the cultivated parts of Sanghuangporus vaninii under the forest. Its primary and advanced structure was analyzed by a series of techniques including GC-MS, methylation, NMR, MALS as well as AFM. The results indicated that...

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Autores principales: Cheng, Junwen, Wang, Yanbin, Song, Jiling, Liu, Yu, Ji, Weiwei, He, Liang, Wei, Hailong, Hu, Chuanjiu, Jiang, Yihan, Xing, Yiqi, Huang, Xubo, Ding, Hongmei, He, Qinghai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9812957/
https://www.ncbi.nlm.nih.gov/pubmed/36618684
http://dx.doi.org/10.3389/fnut.2022.1058131
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author Cheng, Junwen
Wang, Yanbin
Song, Jiling
Liu, Yu
Ji, Weiwei
He, Liang
Wei, Hailong
Hu, Chuanjiu
Jiang, Yihan
Xing, Yiqi
Huang, Xubo
Ding, Hongmei
He, Qinghai
author_facet Cheng, Junwen
Wang, Yanbin
Song, Jiling
Liu, Yu
Ji, Weiwei
He, Liang
Wei, Hailong
Hu, Chuanjiu
Jiang, Yihan
Xing, Yiqi
Huang, Xubo
Ding, Hongmei
He, Qinghai
author_sort Cheng, Junwen
collection PubMed
description A biomacromolecule, named as β-galactoglucofurannan (SVPS2), was isolated from the cultivated parts of Sanghuangporus vaninii under the forest. Its primary and advanced structure was analyzed by a series of techniques including GC-MS, methylation, NMR, MALS as well as AFM. The results indicated that SVPS2 was a kind of 1, 5-linked β-Glucofurannan consisting of β-glucose, β-galactose and α-fucose with 23.4 KDa. It exhibited a single-stranded chain with an average height of 0.72 nm in saline solution. The immunostimulation test indicated SVPS2 could facilitate the initiation of the immune reaction and promote the secretion of cytokines in vitro. Moreover, SVPS2 could mediate the apoptosis of HT-29 cells by blocking them in S phase. Western blot assay revealed an upregulation of Bax, Cytochrome c and cleaved caspase-3 by SVPS2, accompanied by a downregulation of Bcl-2. These results collectively demonstrate that antitumor mechanism of SVPS2 may be associated with enhancing immune response and inducing apoptosis of tumor cells in vitro. Therefore, SVPS2 might be utilized as a promising therapeutic agent against colon cancer and functional food with immunomodulatory activity.
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spelling pubmed-98129572023-01-06 Characterization, immunostimulatory and antitumor activities of a β-galactoglucofurannan from cultivated Sanghuangporus vaninii under forest Cheng, Junwen Wang, Yanbin Song, Jiling Liu, Yu Ji, Weiwei He, Liang Wei, Hailong Hu, Chuanjiu Jiang, Yihan Xing, Yiqi Huang, Xubo Ding, Hongmei He, Qinghai Front Nutr Nutrition A biomacromolecule, named as β-galactoglucofurannan (SVPS2), was isolated from the cultivated parts of Sanghuangporus vaninii under the forest. Its primary and advanced structure was analyzed by a series of techniques including GC-MS, methylation, NMR, MALS as well as AFM. The results indicated that SVPS2 was a kind of 1, 5-linked β-Glucofurannan consisting of β-glucose, β-galactose and α-fucose with 23.4 KDa. It exhibited a single-stranded chain with an average height of 0.72 nm in saline solution. The immunostimulation test indicated SVPS2 could facilitate the initiation of the immune reaction and promote the secretion of cytokines in vitro. Moreover, SVPS2 could mediate the apoptosis of HT-29 cells by blocking them in S phase. Western blot assay revealed an upregulation of Bax, Cytochrome c and cleaved caspase-3 by SVPS2, accompanied by a downregulation of Bcl-2. These results collectively demonstrate that antitumor mechanism of SVPS2 may be associated with enhancing immune response and inducing apoptosis of tumor cells in vitro. Therefore, SVPS2 might be utilized as a promising therapeutic agent against colon cancer and functional food with immunomodulatory activity. Frontiers Media S.A. 2022-12-22 /pmc/articles/PMC9812957/ /pubmed/36618684 http://dx.doi.org/10.3389/fnut.2022.1058131 Text en Copyright © 2022 Cheng, Wang, Song, Liu, Ji, He, Wei, Hu, Jiang, Xing, Huang, Ding and He. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Cheng, Junwen
Wang, Yanbin
Song, Jiling
Liu, Yu
Ji, Weiwei
He, Liang
Wei, Hailong
Hu, Chuanjiu
Jiang, Yihan
Xing, Yiqi
Huang, Xubo
Ding, Hongmei
He, Qinghai
Characterization, immunostimulatory and antitumor activities of a β-galactoglucofurannan from cultivated Sanghuangporus vaninii under forest
title Characterization, immunostimulatory and antitumor activities of a β-galactoglucofurannan from cultivated Sanghuangporus vaninii under forest
title_full Characterization, immunostimulatory and antitumor activities of a β-galactoglucofurannan from cultivated Sanghuangporus vaninii under forest
title_fullStr Characterization, immunostimulatory and antitumor activities of a β-galactoglucofurannan from cultivated Sanghuangporus vaninii under forest
title_full_unstemmed Characterization, immunostimulatory and antitumor activities of a β-galactoglucofurannan from cultivated Sanghuangporus vaninii under forest
title_short Characterization, immunostimulatory and antitumor activities of a β-galactoglucofurannan from cultivated Sanghuangporus vaninii under forest
title_sort characterization, immunostimulatory and antitumor activities of a β-galactoglucofurannan from cultivated sanghuangporus vaninii under forest
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9812957/
https://www.ncbi.nlm.nih.gov/pubmed/36618684
http://dx.doi.org/10.3389/fnut.2022.1058131
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