Detection of apoptosis by [(18)F]ML-10 after cardiac ischemia–reperfusion injury in mice

OBJECTIVE: Myocardial infarction leads to ischemic heart disease and cell death, which is still a major obstacle in western society. In vivo imaging of apoptosis, a defined cascade of cell death, could identify myocardial tissue at risk. METHODS: Using 2-(5-[(18)F]fluoropentyl)-2-methyl-malonic acid...

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Autores principales: Fischer, Maximilian, Zacherl, Mathias J., Olivier, Jessica, Lindner, Simon, Massberg, Steffen, Bartenstein, Peter, Grawe, Freba, Ziegler, Sibylle, Brendel, Matthias, Lehner, Sebastian, Boening, Guido, Todica, Andrei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Nature Singapore 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813199/
https://www.ncbi.nlm.nih.gov/pubmed/36306025
http://dx.doi.org/10.1007/s12149-022-01801-0
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author Fischer, Maximilian
Zacherl, Mathias J.
Olivier, Jessica
Lindner, Simon
Massberg, Steffen
Bartenstein, Peter
Grawe, Freba
Ziegler, Sibylle
Brendel, Matthias
Lehner, Sebastian
Boening, Guido
Todica, Andrei
author_facet Fischer, Maximilian
Zacherl, Mathias J.
Olivier, Jessica
Lindner, Simon
Massberg, Steffen
Bartenstein, Peter
Grawe, Freba
Ziegler, Sibylle
Brendel, Matthias
Lehner, Sebastian
Boening, Guido
Todica, Andrei
author_sort Fischer, Maximilian
collection PubMed
description OBJECTIVE: Myocardial infarction leads to ischemic heart disease and cell death, which is still a major obstacle in western society. In vivo imaging of apoptosis, a defined cascade of cell death, could identify myocardial tissue at risk. METHODS: Using 2-(5-[(18)F]fluoropentyl)-2-methyl-malonic acid ([(18)F]ML-10) in autoradiography and positron emission tomography (PET) visualized apoptosis in a mouse model of transient ligation of the left anterior descending (LAD) artery. 2-deoxy-2-[18F]fluoro-D-glucose ([(18)F]FDG) PET imaging indicated the defect area. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) histology stain indicated cardiac apoptosis. RESULTS: [(18)F]ML-10 uptake was evident in the ischemic area after transient LAD ligation in ex vivo autoradiography and in vivo PET imaging. Detection of [(18)F]ML-10 is in line with the defect visualized by [(18)F]FDG and the histological approach of TUNEL staining. CONCLUSION: The tracer [(18)F]ML-10 is suitable for detecting apoptosis after transient LAD ligation in mice.
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spelling pubmed-98131992023-01-06 Detection of apoptosis by [(18)F]ML-10 after cardiac ischemia–reperfusion injury in mice Fischer, Maximilian Zacherl, Mathias J. Olivier, Jessica Lindner, Simon Massberg, Steffen Bartenstein, Peter Grawe, Freba Ziegler, Sibylle Brendel, Matthias Lehner, Sebastian Boening, Guido Todica, Andrei Ann Nucl Med Original Article OBJECTIVE: Myocardial infarction leads to ischemic heart disease and cell death, which is still a major obstacle in western society. In vivo imaging of apoptosis, a defined cascade of cell death, could identify myocardial tissue at risk. METHODS: Using 2-(5-[(18)F]fluoropentyl)-2-methyl-malonic acid ([(18)F]ML-10) in autoradiography and positron emission tomography (PET) visualized apoptosis in a mouse model of transient ligation of the left anterior descending (LAD) artery. 2-deoxy-2-[18F]fluoro-D-glucose ([(18)F]FDG) PET imaging indicated the defect area. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) histology stain indicated cardiac apoptosis. RESULTS: [(18)F]ML-10 uptake was evident in the ischemic area after transient LAD ligation in ex vivo autoradiography and in vivo PET imaging. Detection of [(18)F]ML-10 is in line with the defect visualized by [(18)F]FDG and the histological approach of TUNEL staining. CONCLUSION: The tracer [(18)F]ML-10 is suitable for detecting apoptosis after transient LAD ligation in mice. Springer Nature Singapore 2022-10-28 2023 /pmc/articles/PMC9813199/ /pubmed/36306025 http://dx.doi.org/10.1007/s12149-022-01801-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Fischer, Maximilian
Zacherl, Mathias J.
Olivier, Jessica
Lindner, Simon
Massberg, Steffen
Bartenstein, Peter
Grawe, Freba
Ziegler, Sibylle
Brendel, Matthias
Lehner, Sebastian
Boening, Guido
Todica, Andrei
Detection of apoptosis by [(18)F]ML-10 after cardiac ischemia–reperfusion injury in mice
title Detection of apoptosis by [(18)F]ML-10 after cardiac ischemia–reperfusion injury in mice
title_full Detection of apoptosis by [(18)F]ML-10 after cardiac ischemia–reperfusion injury in mice
title_fullStr Detection of apoptosis by [(18)F]ML-10 after cardiac ischemia–reperfusion injury in mice
title_full_unstemmed Detection of apoptosis by [(18)F]ML-10 after cardiac ischemia–reperfusion injury in mice
title_short Detection of apoptosis by [(18)F]ML-10 after cardiac ischemia–reperfusion injury in mice
title_sort detection of apoptosis by [(18)f]ml-10 after cardiac ischemia–reperfusion injury in mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813199/
https://www.ncbi.nlm.nih.gov/pubmed/36306025
http://dx.doi.org/10.1007/s12149-022-01801-0
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