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Diminished schwann cell repair responses play a role in delayed diabetes-associated wound healing
Diabetes mellitus is the most common metabolic disease associated with impaired wound healing. Recently, Schwann cells (SCs), the glia of the peripheral nervous system, have been suggested to accelerate normal skin wound healing. However, the roles of SCs in diabetic wound healing are not fully unde...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813439/ https://www.ncbi.nlm.nih.gov/pubmed/36620211 http://dx.doi.org/10.3389/fphys.2022.814754 |
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author | Zhou, Shaolong Wan, Lingling Liu, Xu Hu, Delin Lu, Feng Chen, Xihang Liang, Fangguo |
author_facet | Zhou, Shaolong Wan, Lingling Liu, Xu Hu, Delin Lu, Feng Chen, Xihang Liang, Fangguo |
author_sort | Zhou, Shaolong |
collection | PubMed |
description | Diabetes mellitus is the most common metabolic disease associated with impaired wound healing. Recently, Schwann cells (SCs), the glia of the peripheral nervous system, have been suggested to accelerate normal skin wound healing. However, the roles of SCs in diabetic wound healing are not fully understood. In this study, Full-thickness wounds were made in the dorsal skin of C57/B6 mice and db/db (diabetic) mice. Tissue samples were collected at different time points, and immunohistochemical and immunofluorescence analyses were performed to detect markers of de-differentiated SCs, including myelin basic protein, Sox 10, p75, c-Jun, and Ki67. In addition, in vitro experiments were performed using rat SC (RSC96) and murine fibroblast (L929) cell lines to examine the effects of high glucose conditions (50 mM) on the de-differentiation of SCs and the paracrine effects of SCs on myofibroblast formation. Here, we found that, compared with that in normal mice, wound healing was delayed and SCs failed to rapidly activate a repair program after skin wound injury in diabetic mice. Furthermore, we found that SCs from diabetic mice displayed functional impairments in cell de-differentiation, cell-cycle re-entry, and cell migration. In vitro, hyperglycemia impaired RSC 96 cell de-differentiation, cell-cycle re-entry, and cell migration, as well as their paracrine effects on myofibroblast formation, including the secretion of TGF-β and Timp1. These results suggest that delayed wound healing in diabetes is due in part to a diminished SC repair response and attenuated paracrine effects on myofibroblast formation. |
format | Online Article Text |
id | pubmed-9813439 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98134392023-01-06 Diminished schwann cell repair responses play a role in delayed diabetes-associated wound healing Zhou, Shaolong Wan, Lingling Liu, Xu Hu, Delin Lu, Feng Chen, Xihang Liang, Fangguo Front Physiol Physiology Diabetes mellitus is the most common metabolic disease associated with impaired wound healing. Recently, Schwann cells (SCs), the glia of the peripheral nervous system, have been suggested to accelerate normal skin wound healing. However, the roles of SCs in diabetic wound healing are not fully understood. In this study, Full-thickness wounds were made in the dorsal skin of C57/B6 mice and db/db (diabetic) mice. Tissue samples were collected at different time points, and immunohistochemical and immunofluorescence analyses were performed to detect markers of de-differentiated SCs, including myelin basic protein, Sox 10, p75, c-Jun, and Ki67. In addition, in vitro experiments were performed using rat SC (RSC96) and murine fibroblast (L929) cell lines to examine the effects of high glucose conditions (50 mM) on the de-differentiation of SCs and the paracrine effects of SCs on myofibroblast formation. Here, we found that, compared with that in normal mice, wound healing was delayed and SCs failed to rapidly activate a repair program after skin wound injury in diabetic mice. Furthermore, we found that SCs from diabetic mice displayed functional impairments in cell de-differentiation, cell-cycle re-entry, and cell migration. In vitro, hyperglycemia impaired RSC 96 cell de-differentiation, cell-cycle re-entry, and cell migration, as well as their paracrine effects on myofibroblast formation, including the secretion of TGF-β and Timp1. These results suggest that delayed wound healing in diabetes is due in part to a diminished SC repair response and attenuated paracrine effects on myofibroblast formation. Frontiers Media S.A. 2022-12-22 /pmc/articles/PMC9813439/ /pubmed/36620211 http://dx.doi.org/10.3389/fphys.2022.814754 Text en Copyright © 2022 Zhou, Wan, Liu, Hu, Lu, Chen and Liang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Zhou, Shaolong Wan, Lingling Liu, Xu Hu, Delin Lu, Feng Chen, Xihang Liang, Fangguo Diminished schwann cell repair responses play a role in delayed diabetes-associated wound healing |
title | Diminished schwann cell repair responses play a role in delayed diabetes-associated wound healing |
title_full | Diminished schwann cell repair responses play a role in delayed diabetes-associated wound healing |
title_fullStr | Diminished schwann cell repair responses play a role in delayed diabetes-associated wound healing |
title_full_unstemmed | Diminished schwann cell repair responses play a role in delayed diabetes-associated wound healing |
title_short | Diminished schwann cell repair responses play a role in delayed diabetes-associated wound healing |
title_sort | diminished schwann cell repair responses play a role in delayed diabetes-associated wound healing |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813439/ https://www.ncbi.nlm.nih.gov/pubmed/36620211 http://dx.doi.org/10.3389/fphys.2022.814754 |
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