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Immobilized cellulose nanospheres enable rapid antigen detection in lateral flow immunoassays
Rapid diagnostic systems are essential in controlling the spread of viral pathogens and efficient patient management. The available technologies for low-cost viral antigen testing have several limitations, including a lack of accuracy and sensitivity. Here, we introduce a platform based on cellulose...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813465/ https://www.ncbi.nlm.nih.gov/pubmed/36624885 http://dx.doi.org/10.1007/s10570-022-05038-y |
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author | Solin, Katariina Beaumont, Marco Borghei, Maryam Orelma, Hannes Mertens, Pascal Rojas, Orlando J. |
author_facet | Solin, Katariina Beaumont, Marco Borghei, Maryam Orelma, Hannes Mertens, Pascal Rojas, Orlando J. |
author_sort | Solin, Katariina |
collection | PubMed |
description | Rapid diagnostic systems are essential in controlling the spread of viral pathogens and efficient patient management. The available technologies for low-cost viral antigen testing have several limitations, including a lack of accuracy and sensitivity. Here, we introduce a platform based on cellulose II nanoparticles (oppositely charged NPan and NPcat) for effective control of surface protein interactions, leading to rapid and sensitive antigen tests. Passivation against non-specific adsorption and augmented immobilization of sensing antibodies is achieved by adjusting the electrostatic charge of the nanoparticles. The interactions affecting the performance of the system are investigated by microgravimetry and confocal imaging. As a proof-of-concept test, SARS-CoV-2 nucleocapsid sensing was carried out by using saliva-wicking by channels that were stencil-printed on paper. We conclude that inkjet-printed NPcat elicits strong optical signals, visible after a few minutes, opening the opportunity for cost-effective and rapid diagnostic. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10570-022-05038-y. |
format | Online Article Text |
id | pubmed-9813465 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-98134652023-01-05 Immobilized cellulose nanospheres enable rapid antigen detection in lateral flow immunoassays Solin, Katariina Beaumont, Marco Borghei, Maryam Orelma, Hannes Mertens, Pascal Rojas, Orlando J. Cellulose (Lond) Original Research Rapid diagnostic systems are essential in controlling the spread of viral pathogens and efficient patient management. The available technologies for low-cost viral antigen testing have several limitations, including a lack of accuracy and sensitivity. Here, we introduce a platform based on cellulose II nanoparticles (oppositely charged NPan and NPcat) for effective control of surface protein interactions, leading to rapid and sensitive antigen tests. Passivation against non-specific adsorption and augmented immobilization of sensing antibodies is achieved by adjusting the electrostatic charge of the nanoparticles. The interactions affecting the performance of the system are investigated by microgravimetry and confocal imaging. As a proof-of-concept test, SARS-CoV-2 nucleocapsid sensing was carried out by using saliva-wicking by channels that were stencil-printed on paper. We conclude that inkjet-printed NPcat elicits strong optical signals, visible after a few minutes, opening the opportunity for cost-effective and rapid diagnostic. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10570-022-05038-y. Springer Netherlands 2023-01-05 2023 /pmc/articles/PMC9813465/ /pubmed/36624885 http://dx.doi.org/10.1007/s10570-022-05038-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Research Solin, Katariina Beaumont, Marco Borghei, Maryam Orelma, Hannes Mertens, Pascal Rojas, Orlando J. Immobilized cellulose nanospheres enable rapid antigen detection in lateral flow immunoassays |
title | Immobilized cellulose nanospheres enable rapid antigen detection in lateral flow immunoassays |
title_full | Immobilized cellulose nanospheres enable rapid antigen detection in lateral flow immunoassays |
title_fullStr | Immobilized cellulose nanospheres enable rapid antigen detection in lateral flow immunoassays |
title_full_unstemmed | Immobilized cellulose nanospheres enable rapid antigen detection in lateral flow immunoassays |
title_short | Immobilized cellulose nanospheres enable rapid antigen detection in lateral flow immunoassays |
title_sort | immobilized cellulose nanospheres enable rapid antigen detection in lateral flow immunoassays |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813465/ https://www.ncbi.nlm.nih.gov/pubmed/36624885 http://dx.doi.org/10.1007/s10570-022-05038-y |
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