Identification of a novel linear B-cell epitope in porcine deltacoronavirus nucleocapsid protein

ABSTRACT: Porcine deltacoronavirus (PDCoV) is an emerging swine enteropathogenic coronavirus that caused diarrhea and/or vomiting in neonatal piglets worldwide. Coronaviruses nucleocapsid (N) protein is the most conserved structural protein for viral replication and possesses good antigenicity. In t...

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Autores principales: He, Wei, Shi, Xinze, Guan, Haifei, Zou, Yuntong, Zhang, Shengkun, Jiang, Zhiwen, Su, Shuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813470/
https://www.ncbi.nlm.nih.gov/pubmed/36602561
http://dx.doi.org/10.1007/s00253-022-12348-5
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author He, Wei
Shi, Xinze
Guan, Haifei
Zou, Yuntong
Zhang, Shengkun
Jiang, Zhiwen
Su, Shuo
author_facet He, Wei
Shi, Xinze
Guan, Haifei
Zou, Yuntong
Zhang, Shengkun
Jiang, Zhiwen
Su, Shuo
author_sort He, Wei
collection PubMed
description ABSTRACT: Porcine deltacoronavirus (PDCoV) is an emerging swine enteropathogenic coronavirus that caused diarrhea and/or vomiting in neonatal piglets worldwide. Coronaviruses nucleocapsid (N) protein is the most conserved structural protein for viral replication and possesses good antigenicity. In this study, three monoclonal antibodies (mAbs), 3B4, 4D3, and 4E3 identified as subclass IgG2aκ were prepared using the lymphocytic hybridoma technology against PDCoV N protein. Furthermore, the B-cell epitope recognized by mAb 4D3 was mapped by dozens of overlapping truncated recombinant proteins based on the western blotting. The polypeptide (28)QFRGNGVPLNSAIKPVE(44) (EP-4D3) in the N-terminal of PDCoV N protein was identified as the minimal linear epitope for binding mAb 4D3. And the EP-4D3 epitope’s amino acid sequence homology study revealed that PDCoV strains are substantially conserved, with the exception of the Alanine(43) substitution Valine(43) in the China lineage, the Early China lineage, and the Thailand, Vietnam, and Laos lineage. The epitope sequences shared high similarity (94.1%) with porcine coronavirus HKU15-155 (PorCoV HKU15), Asian leopard cats coronavirus (ALCCoV), sparrow coronavirus HKU17 (SpCoV HKU17), and sparrow deltacoronavirus. In contrast, the epitope sequences shared a very low homology (11.8 to 29.4%) with other porcine CoVs (PEDV, TGEV, PRCV, SADS-CoV, PHEV). Overall, the study will enrich the biological function of PDCoV N protein and provide foundational data for further development of diagnostic applications. KEY POINTS: • Three monoclonal antibodies against PDCoV N protein were prepared. • Discovery of a novel B-cell liner epitope ((28)QFRGNGVPLNSAIKPVE(44)) of PDCoV N protein. • The epitope EP-4D3 was conserved among PDCoV strains. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-022-12348-5.
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spelling pubmed-98134702023-01-05 Identification of a novel linear B-cell epitope in porcine deltacoronavirus nucleocapsid protein He, Wei Shi, Xinze Guan, Haifei Zou, Yuntong Zhang, Shengkun Jiang, Zhiwen Su, Shuo Appl Microbiol Biotechnol Biotechnological Products and Process Engineering ABSTRACT: Porcine deltacoronavirus (PDCoV) is an emerging swine enteropathogenic coronavirus that caused diarrhea and/or vomiting in neonatal piglets worldwide. Coronaviruses nucleocapsid (N) protein is the most conserved structural protein for viral replication and possesses good antigenicity. In this study, three monoclonal antibodies (mAbs), 3B4, 4D3, and 4E3 identified as subclass IgG2aκ were prepared using the lymphocytic hybridoma technology against PDCoV N protein. Furthermore, the B-cell epitope recognized by mAb 4D3 was mapped by dozens of overlapping truncated recombinant proteins based on the western blotting. The polypeptide (28)QFRGNGVPLNSAIKPVE(44) (EP-4D3) in the N-terminal of PDCoV N protein was identified as the minimal linear epitope for binding mAb 4D3. And the EP-4D3 epitope’s amino acid sequence homology study revealed that PDCoV strains are substantially conserved, with the exception of the Alanine(43) substitution Valine(43) in the China lineage, the Early China lineage, and the Thailand, Vietnam, and Laos lineage. The epitope sequences shared high similarity (94.1%) with porcine coronavirus HKU15-155 (PorCoV HKU15), Asian leopard cats coronavirus (ALCCoV), sparrow coronavirus HKU17 (SpCoV HKU17), and sparrow deltacoronavirus. In contrast, the epitope sequences shared a very low homology (11.8 to 29.4%) with other porcine CoVs (PEDV, TGEV, PRCV, SADS-CoV, PHEV). Overall, the study will enrich the biological function of PDCoV N protein and provide foundational data for further development of diagnostic applications. KEY POINTS: • Three monoclonal antibodies against PDCoV N protein were prepared. • Discovery of a novel B-cell liner epitope ((28)QFRGNGVPLNSAIKPVE(44)) of PDCoV N protein. • The epitope EP-4D3 was conserved among PDCoV strains. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-022-12348-5. Springer Berlin Heidelberg 2023-01-05 2023 /pmc/articles/PMC9813470/ /pubmed/36602561 http://dx.doi.org/10.1007/s00253-022-12348-5 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Biotechnological Products and Process Engineering
He, Wei
Shi, Xinze
Guan, Haifei
Zou, Yuntong
Zhang, Shengkun
Jiang, Zhiwen
Su, Shuo
Identification of a novel linear B-cell epitope in porcine deltacoronavirus nucleocapsid protein
title Identification of a novel linear B-cell epitope in porcine deltacoronavirus nucleocapsid protein
title_full Identification of a novel linear B-cell epitope in porcine deltacoronavirus nucleocapsid protein
title_fullStr Identification of a novel linear B-cell epitope in porcine deltacoronavirus nucleocapsid protein
title_full_unstemmed Identification of a novel linear B-cell epitope in porcine deltacoronavirus nucleocapsid protein
title_short Identification of a novel linear B-cell epitope in porcine deltacoronavirus nucleocapsid protein
title_sort identification of a novel linear b-cell epitope in porcine deltacoronavirus nucleocapsid protein
topic Biotechnological Products and Process Engineering
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813470/
https://www.ncbi.nlm.nih.gov/pubmed/36602561
http://dx.doi.org/10.1007/s00253-022-12348-5
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