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Anesthetic-specific lncRNA and mRNA profile changes in blood during colorectal cancer resection: A prospective, matched-case pilot study

Prometastatic and antitumor effects of different anesthetics have been previously analyzed in several studies with conflicting results. Thus, the underlying perioperative molecular mechanisms mediated by anesthetics potentially affecting tumor phenotype and metastasis remain unclear. It was hypothes...

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Autores principales: Lindemann, Anja, Brandes, Florian, Borrmann, Melanie, Meidert, Agnes S., Kirchner, Benedikt, Steinlein, Ortrud K., Schelling, Gustav, Pfaffl, Michael W., Reithmair, Marlene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813548/
https://www.ncbi.nlm.nih.gov/pubmed/36562401
http://dx.doi.org/10.3892/or.2022.8465
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author Lindemann, Anja
Brandes, Florian
Borrmann, Melanie
Meidert, Agnes S.
Kirchner, Benedikt
Steinlein, Ortrud K.
Schelling, Gustav
Pfaffl, Michael W.
Reithmair, Marlene
author_facet Lindemann, Anja
Brandes, Florian
Borrmann, Melanie
Meidert, Agnes S.
Kirchner, Benedikt
Steinlein, Ortrud K.
Schelling, Gustav
Pfaffl, Michael W.
Reithmair, Marlene
author_sort Lindemann, Anja
collection PubMed
description Prometastatic and antitumor effects of different anesthetics have been previously analyzed in several studies with conflicting results. Thus, the underlying perioperative molecular mechanisms mediated by anesthetics potentially affecting tumor phenotype and metastasis remain unclear. It was hypothesized that anesthetic-specific long non-coding RNA (lncRNA) expression changes are induced in the blood circulation and play a crucial role in tumor outcome. In the present study, high-throughput sequencing and quantitative PCR were performed in order to identify lncRNA and mRNA expression changes affected by two therapeutic regimes, total intravenous anesthesia (TIVA) and volatile anesthetic gas (VAG) in patients undergoing colorectal cancer (CRC) resection. Total blood RNA was isolated prior to and following resection and characterized using RNA sequencing. mRNA-lncRNA interactions and their roles in cancer-related signaling of differentially expressed lncRNAs were identified using bioinformatics analyses. The comparison of these two time points revealed 35 differentially expressed lncRNAs in the TIVA-group, and 25 in the VAG-group, whereas eight were shared by both groups. Two lncRNAs in the TIVA-group, and 23 in the VAG-group of in silico identified target-mRNAs were confirmed as differentially regulated in the NGS dataset of the present study. Pathway analysis was performed and cancer relevant canonical pathways for TIVA were identified. Target-mRNA analysis of VAG revealed a markedly worsened immunological response against cancer. In this proof-of-concept study, anesthesic-specific expression changes in lncRNA and mRNA profiles in blood were successfully identified. Moreover, the data of the present study provide the first evidence that anesthesia-induced lncRNA pattern changes may contribute further in the observed differences in CRC outcome following tumor resection.
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spelling pubmed-98135482023-01-12 Anesthetic-specific lncRNA and mRNA profile changes in blood during colorectal cancer resection: A prospective, matched-case pilot study Lindemann, Anja Brandes, Florian Borrmann, Melanie Meidert, Agnes S. Kirchner, Benedikt Steinlein, Ortrud K. Schelling, Gustav Pfaffl, Michael W. Reithmair, Marlene Oncol Rep Articles Prometastatic and antitumor effects of different anesthetics have been previously analyzed in several studies with conflicting results. Thus, the underlying perioperative molecular mechanisms mediated by anesthetics potentially affecting tumor phenotype and metastasis remain unclear. It was hypothesized that anesthetic-specific long non-coding RNA (lncRNA) expression changes are induced in the blood circulation and play a crucial role in tumor outcome. In the present study, high-throughput sequencing and quantitative PCR were performed in order to identify lncRNA and mRNA expression changes affected by two therapeutic regimes, total intravenous anesthesia (TIVA) and volatile anesthetic gas (VAG) in patients undergoing colorectal cancer (CRC) resection. Total blood RNA was isolated prior to and following resection and characterized using RNA sequencing. mRNA-lncRNA interactions and their roles in cancer-related signaling of differentially expressed lncRNAs were identified using bioinformatics analyses. The comparison of these two time points revealed 35 differentially expressed lncRNAs in the TIVA-group, and 25 in the VAG-group, whereas eight were shared by both groups. Two lncRNAs in the TIVA-group, and 23 in the VAG-group of in silico identified target-mRNAs were confirmed as differentially regulated in the NGS dataset of the present study. Pathway analysis was performed and cancer relevant canonical pathways for TIVA were identified. Target-mRNA analysis of VAG revealed a markedly worsened immunological response against cancer. In this proof-of-concept study, anesthesic-specific expression changes in lncRNA and mRNA profiles in blood were successfully identified. Moreover, the data of the present study provide the first evidence that anesthesia-induced lncRNA pattern changes may contribute further in the observed differences in CRC outcome following tumor resection. D.A. Spandidos 2022-12-16 /pmc/articles/PMC9813548/ /pubmed/36562401 http://dx.doi.org/10.3892/or.2022.8465 Text en Copyright: © Lindemann et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Lindemann, Anja
Brandes, Florian
Borrmann, Melanie
Meidert, Agnes S.
Kirchner, Benedikt
Steinlein, Ortrud K.
Schelling, Gustav
Pfaffl, Michael W.
Reithmair, Marlene
Anesthetic-specific lncRNA and mRNA profile changes in blood during colorectal cancer resection: A prospective, matched-case pilot study
title Anesthetic-specific lncRNA and mRNA profile changes in blood during colorectal cancer resection: A prospective, matched-case pilot study
title_full Anesthetic-specific lncRNA and mRNA profile changes in blood during colorectal cancer resection: A prospective, matched-case pilot study
title_fullStr Anesthetic-specific lncRNA and mRNA profile changes in blood during colorectal cancer resection: A prospective, matched-case pilot study
title_full_unstemmed Anesthetic-specific lncRNA and mRNA profile changes in blood during colorectal cancer resection: A prospective, matched-case pilot study
title_short Anesthetic-specific lncRNA and mRNA profile changes in blood during colorectal cancer resection: A prospective, matched-case pilot study
title_sort anesthetic-specific lncrna and mrna profile changes in blood during colorectal cancer resection: a prospective, matched-case pilot study
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813548/
https://www.ncbi.nlm.nih.gov/pubmed/36562401
http://dx.doi.org/10.3892/or.2022.8465
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