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Plastidic membrane lipids are oxidized by a lipoxygenase in Lobosphaera incisa
Green microalgae can accumulate neutral lipids, as part of a general lipid remodeling mechanism under stress such as nitrogen starvation. Lobosphaera incisa is of special interest because of its unique TAG acyl chain composition, especially 20:4 (n-6) can reach up to 21% of dry weight after nitrogen...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813749/ https://www.ncbi.nlm.nih.gov/pubmed/36618660 http://dx.doi.org/10.3389/fpls.2022.1102215 |
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author | Djian, Benjamin Feussner, Kirstin Herrfurth, Cornelia Zienkiewicz, Krzysztof Hornung, Ellen Feussner, Ivo |
author_facet | Djian, Benjamin Feussner, Kirstin Herrfurth, Cornelia Zienkiewicz, Krzysztof Hornung, Ellen Feussner, Ivo |
author_sort | Djian, Benjamin |
collection | PubMed |
description | Green microalgae can accumulate neutral lipids, as part of a general lipid remodeling mechanism under stress such as nitrogen starvation. Lobosphaera incisa is of special interest because of its unique TAG acyl chain composition, especially 20:4 (n-6) can reach up to 21% of dry weight after nitrogen starvation. In order to identify factors that may influence the accumulation of polyunsaturated fatty acids (PUFAs), we identified recently a linoleate 13-lipoxygenase (LiLOX). It shares highest identity with plastidic enzymes from vascular plants and is induced upon nitrogen starvation. Here, we confirmed the localization of LiLOX in the stroma of plastids via transient expression in epithelial onion cells. In order to further characterize this enzyme, we focused on the identification of the endogenous substrate of LiLOX. In this regard, an ex vivo enzymatic assay, coupled with non-targeted analysis via mass spectrometry allowed the identification of MGDG, DGDG and PC as three substrate candidates, later confirmed via in vitro assays. Further investigation revealed that LiLOX has preferences towards the lipid class MGDG, which seems in agreement with its localization in the galactolipid rich plastid. Altogether, this study shows the first characterization of plastidic LOX from green algae, showing preference for MGDGs. However, lipidomics analysis did neither reveal an endogenous LiLOX product nor the final end product of MGDG oxidation. Nevertheless, the latter is a key to understanding the role of this enzyme and since its expression is highest during the degradation of the plastidic membrane, it is tempting to assume its involvement in this process. |
format | Online Article Text |
id | pubmed-9813749 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98137492023-01-06 Plastidic membrane lipids are oxidized by a lipoxygenase in Lobosphaera incisa Djian, Benjamin Feussner, Kirstin Herrfurth, Cornelia Zienkiewicz, Krzysztof Hornung, Ellen Feussner, Ivo Front Plant Sci Plant Science Green microalgae can accumulate neutral lipids, as part of a general lipid remodeling mechanism under stress such as nitrogen starvation. Lobosphaera incisa is of special interest because of its unique TAG acyl chain composition, especially 20:4 (n-6) can reach up to 21% of dry weight after nitrogen starvation. In order to identify factors that may influence the accumulation of polyunsaturated fatty acids (PUFAs), we identified recently a linoleate 13-lipoxygenase (LiLOX). It shares highest identity with plastidic enzymes from vascular plants and is induced upon nitrogen starvation. Here, we confirmed the localization of LiLOX in the stroma of plastids via transient expression in epithelial onion cells. In order to further characterize this enzyme, we focused on the identification of the endogenous substrate of LiLOX. In this regard, an ex vivo enzymatic assay, coupled with non-targeted analysis via mass spectrometry allowed the identification of MGDG, DGDG and PC as three substrate candidates, later confirmed via in vitro assays. Further investigation revealed that LiLOX has preferences towards the lipid class MGDG, which seems in agreement with its localization in the galactolipid rich plastid. Altogether, this study shows the first characterization of plastidic LOX from green algae, showing preference for MGDGs. However, lipidomics analysis did neither reveal an endogenous LiLOX product nor the final end product of MGDG oxidation. Nevertheless, the latter is a key to understanding the role of this enzyme and since its expression is highest during the degradation of the plastidic membrane, it is tempting to assume its involvement in this process. Frontiers Media S.A. 2022-12-22 /pmc/articles/PMC9813749/ /pubmed/36618660 http://dx.doi.org/10.3389/fpls.2022.1102215 Text en Copyright © 2022 Djian, Feussner, Herrfurth, Zienkiewicz, Hornung and Feussner https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Djian, Benjamin Feussner, Kirstin Herrfurth, Cornelia Zienkiewicz, Krzysztof Hornung, Ellen Feussner, Ivo Plastidic membrane lipids are oxidized by a lipoxygenase in Lobosphaera incisa |
title | Plastidic membrane lipids are oxidized by a lipoxygenase in Lobosphaera incisa
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title_full | Plastidic membrane lipids are oxidized by a lipoxygenase in Lobosphaera incisa
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title_fullStr | Plastidic membrane lipids are oxidized by a lipoxygenase in Lobosphaera incisa
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title_full_unstemmed | Plastidic membrane lipids are oxidized by a lipoxygenase in Lobosphaera incisa
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title_short | Plastidic membrane lipids are oxidized by a lipoxygenase in Lobosphaera incisa
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title_sort | plastidic membrane lipids are oxidized by a lipoxygenase in lobosphaera incisa |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813749/ https://www.ncbi.nlm.nih.gov/pubmed/36618660 http://dx.doi.org/10.3389/fpls.2022.1102215 |
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