Quantitative trait loci and candidate genes for iron and zinc bio-fortification in genetically diverse germplasm of maize (Zea mays L): A systematic review

Genetically and economically, Maize plays a pivotal role in tackling Iron–Zinc mineral deficiency through the crop's biofortification approach to high-yielding cultivars. The objective of this study is to summarize quantitative trait loci (QTL) is useful for identifying novel genes of interest in diverse germplasm for understanding the exact genetic mechanism for Iron and zinc uptake, deposition, and biosynthesis in Zea mays L endosperm. various techniques like Germplasm Genetic Wide Association, QTL meta-analysis, and biparental linkage analysis are used by researchers in diverse germplasm of Maize for the gene of interest marking and are extracted as secondary information through a systematic review of scientific published sources in peer-reviewed sites. A literature review was focused on quantitative trait loci with candidate genes from different families like YS, NRAMP, ferritin, Cation efflux, etc., and cloned four phytase soluble genes which influence the concentration as well as bioavailability of Fe & Zn in the endosperm. More than 30 QTLs with 15-Fe, 17-Zn; 10 Meta QTLS are common and linked with micronutrient concentration as well 17 candidate genes from different families are responsible for the zinc-iron deposition on the endosperm. More than 46 Fe–Zn (20 + 26) SNPs and 22 SNPs (10 + 12) on nine different chromosomes play a significant role in the variation of the mineral value of inbreeds and Double haploid Bi-parental population of Zea mays L. In Rice and Maize, five different chromosomes are collinear for the uptake to deposition of these minerals in the endosperm. The success of marker-based biofortification depends upon the nature of germplasm, the gap between flanking marker and targeted genes, the selection of genotypes in each generation, and genotype-environment interaction which are the future area of study. This study can assist the breeders in fast-tracking Fe and Zn biofortification through frequency multiplication of these desired loci of Maize.