An efficient and cost-effective purification protocol for Staphylococcus aureus Cas9 nuclease

Here, we describe a protocol for purifying functional clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) from Staphylococcus aureus within 24 h and over 90% purity. SaCas9 purification begins with immobilized metal affinity chromatography, followed by cati...

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Detalles Bibliográficos
Autores principales: Teng, Allen C.T., Tavassoli, Marjan, Shrestha, Suja, Marks, Ryan M., McFadden, Meghan J., Evagelou, Sonia L., Lindsay, Kyle, Vandenbelt, Ava, Li, Wenping, Ivakine, Evgueni, Cohn, Ronald, Santerre, J. Paul, Gramolini, Anthony O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813775/
https://www.ncbi.nlm.nih.gov/pubmed/36574341
http://dx.doi.org/10.1016/j.xpro.2022.101933

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