A protocol to detect human CD4(+) T cell extracellular traps using scanning electron microscopy

We present a protocol to detect extracellular traps (ETs) induced by Cutibacterium acnes in cultured T(H)17 clones. We first describe the isolation of C. acnes-specific T(H)17 clones by sterile cell sorting. We then detail the in vitro induction of ETs in T(H)17 clones stimulated by C. acnes and the...

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Detalles Bibliográficos
Autores principales: Nguyen, Nam Phuong N., Oparaugo, Nicole Chizara, Ouyang, Kelsey, Agak, George W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813782/
https://www.ncbi.nlm.nih.gov/pubmed/36574340
http://dx.doi.org/10.1016/j.xpro.2022.101932
Descripción
Sumario:We present a protocol to detect extracellular traps (ETs) induced by Cutibacterium acnes in cultured T(H)17 clones. We first describe the isolation of C. acnes-specific T(H)17 clones by sterile cell sorting. We then detail the in vitro induction of ETs in T(H)17 clones stimulated by C. acnes and the imaging of released ETs using scanning electron microscopy. This protocol can be applied to the study of other ETs released by other T cell subsets. For complete details on the use and execution of this protocol, please refer to Agak et al. (2021).(1)

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