A protocol to detect human CD4(+) T cell extracellular traps using scanning electron microscopy

We present a protocol to detect extracellular traps (ETs) induced by Cutibacterium acnes in cultured T(H)17 clones. We first describe the isolation of C. acnes-specific T(H)17 clones by sterile cell sorting. We then detail the in vitro induction of ETs in T(H)17 clones stimulated by C. acnes and the...

Descripción completa

Detalles Bibliográficos
Autores principales: Nguyen, Nam Phuong N., Oparaugo, Nicole Chizara, Ouyang, Kelsey, Agak, George W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813782/
https://www.ncbi.nlm.nih.gov/pubmed/36574340
http://dx.doi.org/10.1016/j.xpro.2022.101932
_version_ 1784863995349434368
author Nguyen, Nam Phuong N.
Oparaugo, Nicole Chizara
Ouyang, Kelsey
Agak, George W.
author_facet Nguyen, Nam Phuong N.
Oparaugo, Nicole Chizara
Ouyang, Kelsey
Agak, George W.
author_sort Nguyen, Nam Phuong N.
collection PubMed
description We present a protocol to detect extracellular traps (ETs) induced by Cutibacterium acnes in cultured T(H)17 clones. We first describe the isolation of C. acnes-specific T(H)17 clones by sterile cell sorting. We then detail the in vitro induction of ETs in T(H)17 clones stimulated by C. acnes and the imaging of released ETs using scanning electron microscopy. This protocol can be applied to the study of other ETs released by other T cell subsets. For complete details on the use and execution of this protocol, please refer to Agak et al. (2021).(1)
format Online
Article
Text
id pubmed-9813782
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-98137822023-01-06 A protocol to detect human CD4(+) T cell extracellular traps using scanning electron microscopy Nguyen, Nam Phuong N. Oparaugo, Nicole Chizara Ouyang, Kelsey Agak, George W. STAR Protoc Protocol We present a protocol to detect extracellular traps (ETs) induced by Cutibacterium acnes in cultured T(H)17 clones. We first describe the isolation of C. acnes-specific T(H)17 clones by sterile cell sorting. We then detail the in vitro induction of ETs in T(H)17 clones stimulated by C. acnes and the imaging of released ETs using scanning electron microscopy. This protocol can be applied to the study of other ETs released by other T cell subsets. For complete details on the use and execution of this protocol, please refer to Agak et al. (2021).(1) Elsevier 2022-12-26 /pmc/articles/PMC9813782/ /pubmed/36574340 http://dx.doi.org/10.1016/j.xpro.2022.101932 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Nguyen, Nam Phuong N.
Oparaugo, Nicole Chizara
Ouyang, Kelsey
Agak, George W.
A protocol to detect human CD4(+) T cell extracellular traps using scanning electron microscopy
title A protocol to detect human CD4(+) T cell extracellular traps using scanning electron microscopy
title_full A protocol to detect human CD4(+) T cell extracellular traps using scanning electron microscopy
title_fullStr A protocol to detect human CD4(+) T cell extracellular traps using scanning electron microscopy
title_full_unstemmed A protocol to detect human CD4(+) T cell extracellular traps using scanning electron microscopy
title_short A protocol to detect human CD4(+) T cell extracellular traps using scanning electron microscopy
title_sort protocol to detect human cd4(+) t cell extracellular traps using scanning electron microscopy
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813782/
https://www.ncbi.nlm.nih.gov/pubmed/36574340
http://dx.doi.org/10.1016/j.xpro.2022.101932
work_keys_str_mv AT nguyennamphuongn aprotocoltodetecthumancd4tcellextracellulartrapsusingscanningelectronmicroscopy
AT oparaugonicolechizara aprotocoltodetecthumancd4tcellextracellulartrapsusingscanningelectronmicroscopy
AT ouyangkelsey aprotocoltodetecthumancd4tcellextracellulartrapsusingscanningelectronmicroscopy
AT agakgeorgew aprotocoltodetecthumancd4tcellextracellulartrapsusingscanningelectronmicroscopy
AT nguyennamphuongn protocoltodetecthumancd4tcellextracellulartrapsusingscanningelectronmicroscopy
AT oparaugonicolechizara protocoltodetecthumancd4tcellextracellulartrapsusingscanningelectronmicroscopy
AT ouyangkelsey protocoltodetecthumancd4tcellextracellulartrapsusingscanningelectronmicroscopy
AT agakgeorgew protocoltodetecthumancd4tcellextracellulartrapsusingscanningelectronmicroscopy

Ejemplares similares