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A protocol to detect human CD4(+) T cell extracellular traps using scanning electron microscopy

We present a protocol to detect extracellular traps (ETs) induced by Cutibacterium acnes in cultured T(H)17 clones. We first describe the isolation of C. acnes-specific T(H)17 clones by sterile cell sorting. We then detail the in vitro induction of ETs in T(H)17 clones stimulated by C. acnes and the...

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Detalles Bibliográficos
Autores principales:	Nguyen, Nam Phuong N., Oparaugo, Nicole Chizara, Ouyang, Kelsey, Agak, George W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813782/ https://www.ncbi.nlm.nih.gov/pubmed/36574340 http://dx.doi.org/10.1016/j.xpro.2022.101932

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