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Outcomes of nicotinic modulation on markers of intestinal IgA antibody response

Acetylcholine (ACh), as a ligand of nicotinic acetylcholine receptors (nAChRs), plays a key role in the cholinergic anti-inflammatory pathway; however, its role in the immunoglobulin A (IgA) response remains unknown. Therefore, the present study aimed to investigate the role of ACh in the intestinal...

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Autores principales: Carrizales-Luna, Jorge Emmanuel, Reséndiz-Albor, Aldo Arturo, Arciniega-Martínez, Ivonne Maciel, Gómez-López, Modesto, Campos-Rodríguez, Rafael, Pacheco-Yépez, Judith, Drago-Serrano, Maria Elisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813800/
https://www.ncbi.nlm.nih.gov/pubmed/36643694
http://dx.doi.org/10.3892/br.2022.1595
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author Carrizales-Luna, Jorge Emmanuel
Reséndiz-Albor, Aldo Arturo
Arciniega-Martínez, Ivonne Maciel
Gómez-López, Modesto
Campos-Rodríguez, Rafael
Pacheco-Yépez, Judith
Drago-Serrano, Maria Elisa
author_facet Carrizales-Luna, Jorge Emmanuel
Reséndiz-Albor, Aldo Arturo
Arciniega-Martínez, Ivonne Maciel
Gómez-López, Modesto
Campos-Rodríguez, Rafael
Pacheco-Yépez, Judith
Drago-Serrano, Maria Elisa
author_sort Carrizales-Luna, Jorge Emmanuel
collection PubMed
description Acetylcholine (ACh), as a ligand of nicotinic acetylcholine receptors (nAChRs), plays a key role in the cholinergic anti-inflammatory pathway; however, its role in the immunoglobulin A (IgA) response remains unknown. Therefore, the present study aimed to investigate the role of ACh in the intestinal biomarkers involved in IgA synthesis and the polymeric immunoglobulin receptor (pIgR) involved in IgA transcytosis. Groups of mice were administered GTS-21 (an α7nAChR agonist) or mecamylamine (a non-selective nAChR antagonist) intraperitoneally for 7 days. Intestinal fluids were used for antibody concentration assessment by ELISA, cell suspensions from Peyer's patches and the lamina propria were obtained for flow cytometric analysis of plasma cells, and CD4(+) T-cells expressing intracellular transforming growth factor (TGF)-β and IgA-producing interleukin (IL)-4, -5, -6 and -10, and isolated epithelial cells to determine the levels of pIgR mRNA using reverse transcription-quantitative PCR. Regarding to the untreated control group, the concentration of IgA was reduced in the mecamylamine group and unaltered in the GTS-21 group while IgM levels exhibited no differences; the percentage of IgA(+) plasma cells from Peyer's patches and the lamina propria, and the percentage of TGF-β(+)/CD4(+) T-cells from Peyer's patches were greater in the GTS-21-group. In both treatment groups, the percentages of IgM(+) plasma cells and IL-6(+)/IL-10(+) CD4(+) T cells were greater in both compartments; pIgR mRNA expression levels decreased in epithelial cells. The percentage of IL-4 CD4(+) T-cells were greater in Peyer's patches and lower in the lamina propria in the mecamylamine group, and the percentage of IL-5 CD4(+) T-cells in the lamina propria were decreased in both treatment groups. These findings require further examination to address the impact of cholinergic modulation on IgA-transcytosis via pIgR. The present study may be an experimental reference for clinical trials that address the role of nicotinic system in intestinal dysfunctions as postoperative ileus.
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spelling pubmed-98138002023-01-12 Outcomes of nicotinic modulation on markers of intestinal IgA antibody response Carrizales-Luna, Jorge Emmanuel Reséndiz-Albor, Aldo Arturo Arciniega-Martínez, Ivonne Maciel Gómez-López, Modesto Campos-Rodríguez, Rafael Pacheco-Yépez, Judith Drago-Serrano, Maria Elisa Biomed Rep Articles Acetylcholine (ACh), as a ligand of nicotinic acetylcholine receptors (nAChRs), plays a key role in the cholinergic anti-inflammatory pathway; however, its role in the immunoglobulin A (IgA) response remains unknown. Therefore, the present study aimed to investigate the role of ACh in the intestinal biomarkers involved in IgA synthesis and the polymeric immunoglobulin receptor (pIgR) involved in IgA transcytosis. Groups of mice were administered GTS-21 (an α7nAChR agonist) or mecamylamine (a non-selective nAChR antagonist) intraperitoneally for 7 days. Intestinal fluids were used for antibody concentration assessment by ELISA, cell suspensions from Peyer's patches and the lamina propria were obtained for flow cytometric analysis of plasma cells, and CD4(+) T-cells expressing intracellular transforming growth factor (TGF)-β and IgA-producing interleukin (IL)-4, -5, -6 and -10, and isolated epithelial cells to determine the levels of pIgR mRNA using reverse transcription-quantitative PCR. Regarding to the untreated control group, the concentration of IgA was reduced in the mecamylamine group and unaltered in the GTS-21 group while IgM levels exhibited no differences; the percentage of IgA(+) plasma cells from Peyer's patches and the lamina propria, and the percentage of TGF-β(+)/CD4(+) T-cells from Peyer's patches were greater in the GTS-21-group. In both treatment groups, the percentages of IgM(+) plasma cells and IL-6(+)/IL-10(+) CD4(+) T cells were greater in both compartments; pIgR mRNA expression levels decreased in epithelial cells. The percentage of IL-4 CD4(+) T-cells were greater in Peyer's patches and lower in the lamina propria in the mecamylamine group, and the percentage of IL-5 CD4(+) T-cells in the lamina propria were decreased in both treatment groups. These findings require further examination to address the impact of cholinergic modulation on IgA-transcytosis via pIgR. The present study may be an experimental reference for clinical trials that address the role of nicotinic system in intestinal dysfunctions as postoperative ileus. D.A. Spandidos 2022-12-12 /pmc/articles/PMC9813800/ /pubmed/36643694 http://dx.doi.org/10.3892/br.2022.1595 Text en Copyright: © Carrizales-Luna et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Carrizales-Luna, Jorge Emmanuel
Reséndiz-Albor, Aldo Arturo
Arciniega-Martínez, Ivonne Maciel
Gómez-López, Modesto
Campos-Rodríguez, Rafael
Pacheco-Yépez, Judith
Drago-Serrano, Maria Elisa
Outcomes of nicotinic modulation on markers of intestinal IgA antibody response
title Outcomes of nicotinic modulation on markers of intestinal IgA antibody response
title_full Outcomes of nicotinic modulation on markers of intestinal IgA antibody response
title_fullStr Outcomes of nicotinic modulation on markers of intestinal IgA antibody response
title_full_unstemmed Outcomes of nicotinic modulation on markers of intestinal IgA antibody response
title_short Outcomes of nicotinic modulation on markers of intestinal IgA antibody response
title_sort outcomes of nicotinic modulation on markers of intestinal iga antibody response
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813800/
https://www.ncbi.nlm.nih.gov/pubmed/36643694
http://dx.doi.org/10.3892/br.2022.1595
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