Mechanisms Underlying the Antiarrhythmic Action of Compound ALM-802

The mechanisms underlying the antiarrhythmic action of compound trihydrochloride N(1)-(2,3,4-trimethoxy)-N(2)-{2-[(2,3,4-trimethoxybenzyl)amino]ethyl}-1,2-ethane-diamine (code ALM-802) were studied in vitro. The experiments were performed on a culture of rat hippocampal neurons. The electrical activity of neurons was recorded by the patch-clamp method in the whole cell configuration. It is shown that the compound ALM-802 effectively blocks potential-dependent Na(+) and K(+) channels and does not affect the activity of potential-dependent Ca(2+) channels. The inhibition of currents through these channels is dose-dependent; the IC(50) of Na(+) and K(+) channels were 94±4 and 67±3 μM, respectively. These findings indicate that compound ALM-802 combines the properties of class I and class III antiarrhythmic agents according to the Vaughan—Williams classification.