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Joint X-ray/neutron structure of Lentinus similis AA9_A at room temperature
Lytic polysaccharide monooxygenases (LPMOs) are copper metalloenzymes which cleave polysaccharides oxidatively and are important in pathogen biology, carbon cycling and biotechnology. The Lentinus similis family AA9 isoform A (LsAA9_A) has been extensively studied as a model system because its activ...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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International Union of Crystallography
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813973/ https://www.ncbi.nlm.nih.gov/pubmed/36598350 http://dx.doi.org/10.1107/S2053230X22011335 |
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author | Tandrup, Tobias Lo Leggio, Leila Meilleur, Flora |
author_facet | Tandrup, Tobias Lo Leggio, Leila Meilleur, Flora |
author_sort | Tandrup, Tobias |
collection | PubMed |
description | Lytic polysaccharide monooxygenases (LPMOs) are copper metalloenzymes which cleave polysaccharides oxidatively and are important in pathogen biology, carbon cycling and biotechnology. The Lentinus similis family AA9 isoform A (LsAA9_A) has been extensively studied as a model system because its activity towards smaller soluble saccharide substrates has allowed detailed structural characterization of its interaction with a variety of substrates by X-ray crystallography at high resolution. Here, the joint X-ray/neutron room-temperature crystallographic structure of carbohydrate-free LsAA9_A in the copper(II) resting state refined against X-ray and neutron data at 2.1 and 2.8 Å resolution, respectively, is presented. The results provide an experimental determination of the protonation states of the copper(II)-coordinating residues and second-shell residues in LsAA9_A, paving the way for future neutron crystallographic studies of LPMO–carbohydrate complexes. |
format | Online Article Text |
id | pubmed-9813973 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-98139732023-01-09 Joint X-ray/neutron structure of Lentinus similis AA9_A at room temperature Tandrup, Tobias Lo Leggio, Leila Meilleur, Flora Acta Crystallogr F Struct Biol Commun Research Communications Lytic polysaccharide monooxygenases (LPMOs) are copper metalloenzymes which cleave polysaccharides oxidatively and are important in pathogen biology, carbon cycling and biotechnology. The Lentinus similis family AA9 isoform A (LsAA9_A) has been extensively studied as a model system because its activity towards smaller soluble saccharide substrates has allowed detailed structural characterization of its interaction with a variety of substrates by X-ray crystallography at high resolution. Here, the joint X-ray/neutron room-temperature crystallographic structure of carbohydrate-free LsAA9_A in the copper(II) resting state refined against X-ray and neutron data at 2.1 and 2.8 Å resolution, respectively, is presented. The results provide an experimental determination of the protonation states of the copper(II)-coordinating residues and second-shell residues in LsAA9_A, paving the way for future neutron crystallographic studies of LPMO–carbohydrate complexes. International Union of Crystallography 2023-01-01 /pmc/articles/PMC9813973/ /pubmed/36598350 http://dx.doi.org/10.1107/S2053230X22011335 Text en © Tobias Tandrup et al. 2023 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Communications Tandrup, Tobias Lo Leggio, Leila Meilleur, Flora Joint X-ray/neutron structure of Lentinus similis AA9_A at room temperature |
title | Joint X-ray/neutron structure of Lentinus similis AA9_A at room temperature |
title_full | Joint X-ray/neutron structure of Lentinus similis AA9_A at room temperature |
title_fullStr | Joint X-ray/neutron structure of Lentinus similis AA9_A at room temperature |
title_full_unstemmed | Joint X-ray/neutron structure of Lentinus similis AA9_A at room temperature |
title_short | Joint X-ray/neutron structure of Lentinus similis AA9_A at room temperature |
title_sort | joint x-ray/neutron structure of lentinus similis aa9_a at room temperature |
topic | Research Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9813973/ https://www.ncbi.nlm.nih.gov/pubmed/36598350 http://dx.doi.org/10.1107/S2053230X22011335 |
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