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BioSAXS at European Synchrotron Radiation Facility – Extremely Brilliant Source: BM29 with an upgraded source, detector, robot, sample environment, data collection and analysis software
As part of its Extremely Brilliant Source (EBS) upgrade project, the ESRF’s BM29 BioSAXS beamline was subject to a significant upgrade and refurbishment. In addition to the replacement of the beamline’s original bending magnet source by a two-pole wiggler, leading to an increase in brilliance by a f...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9814054/ https://www.ncbi.nlm.nih.gov/pubmed/36601945 http://dx.doi.org/10.1107/S1600577522011286 |
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author | Tully, Mark D. Kieffer, Jerome Brennich, Martha E. Cohen Aberdam, Raphael Florial, Jean Baptiste Hutin, Stephanie Oscarsson, Markus Beteva, Antonia Popov, Anton Moussaoui, Dihia Theveneau, Pascal Papp, Gergely Gigmes, Jonathan Cipriani, Florent McCarthy, Andrew Zubieta, Chloe Mueller-Dieckmann, Christoph Leonard, Gordon Pernot, Petra |
author_facet | Tully, Mark D. Kieffer, Jerome Brennich, Martha E. Cohen Aberdam, Raphael Florial, Jean Baptiste Hutin, Stephanie Oscarsson, Markus Beteva, Antonia Popov, Anton Moussaoui, Dihia Theveneau, Pascal Papp, Gergely Gigmes, Jonathan Cipriani, Florent McCarthy, Andrew Zubieta, Chloe Mueller-Dieckmann, Christoph Leonard, Gordon Pernot, Petra |
author_sort | Tully, Mark D. |
collection | PubMed |
description | As part of its Extremely Brilliant Source (EBS) upgrade project, the ESRF’s BM29 BioSAXS beamline was subject to a significant upgrade and refurbishment. In addition to the replacement of the beamline’s original bending magnet source by a two-pole wiggler, leading to an increase in brilliance by a factor of 60, the sample environment of the beamline was almost completely refurbished: a vacuum-compatible Pilatus3 X 2M with a sensitive area of 253.7 mm × 288 mm and frame rates up to 250 Hz was installed, increasing the active area available and thus the q-scaling of scattering images taken; the sample changer was replaced with an upgraded version, allowing more space for customizable sample environments and the installation of two new sample exposure units; the software associated with the beamline was also renewed. In addition, the layout and functionality of the BSXCuBE3 (BioSAXS Customized Beamline Environment) data acquisition software was redesigned, providing an intuitive ‘user first’ approach for inexperienced users, while at the same time maintaining more powerful options for experienced users and beamline staff. Additional features of BSXCuBE3 are queuing of samples; either consecutive sample changer and/or SEC-SAXS (size-exclusion chromatography small-angle X-ray scattering) experiments, including column equilibration were also implemented. Automatic data processing and analysis are now managed via Dahu, an online server with upstream data reduction, data scaling and azimuthal integration built around PyFAI (Python Fast Azimuthal Integration), and data analysis performed using the open source FreeSAS. The results of this automated data analysis pipeline are displayed in ISPyB/ExiSAXS. The upgraded BM29 has been in operation since the post-EBS restart in September 2020, and here a full description of its new hardware and software characteristics together with examples of data obtained are provided. |
format | Online Article Text |
id | pubmed-9814054 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-98140542023-01-09 BioSAXS at European Synchrotron Radiation Facility – Extremely Brilliant Source: BM29 with an upgraded source, detector, robot, sample environment, data collection and analysis software Tully, Mark D. Kieffer, Jerome Brennich, Martha E. Cohen Aberdam, Raphael Florial, Jean Baptiste Hutin, Stephanie Oscarsson, Markus Beteva, Antonia Popov, Anton Moussaoui, Dihia Theveneau, Pascal Papp, Gergely Gigmes, Jonathan Cipriani, Florent McCarthy, Andrew Zubieta, Chloe Mueller-Dieckmann, Christoph Leonard, Gordon Pernot, Petra J Synchrotron Radiat Beamlines As part of its Extremely Brilliant Source (EBS) upgrade project, the ESRF’s BM29 BioSAXS beamline was subject to a significant upgrade and refurbishment. In addition to the replacement of the beamline’s original bending magnet source by a two-pole wiggler, leading to an increase in brilliance by a factor of 60, the sample environment of the beamline was almost completely refurbished: a vacuum-compatible Pilatus3 X 2M with a sensitive area of 253.7 mm × 288 mm and frame rates up to 250 Hz was installed, increasing the active area available and thus the q-scaling of scattering images taken; the sample changer was replaced with an upgraded version, allowing more space for customizable sample environments and the installation of two new sample exposure units; the software associated with the beamline was also renewed. In addition, the layout and functionality of the BSXCuBE3 (BioSAXS Customized Beamline Environment) data acquisition software was redesigned, providing an intuitive ‘user first’ approach for inexperienced users, while at the same time maintaining more powerful options for experienced users and beamline staff. Additional features of BSXCuBE3 are queuing of samples; either consecutive sample changer and/or SEC-SAXS (size-exclusion chromatography small-angle X-ray scattering) experiments, including column equilibration were also implemented. Automatic data processing and analysis are now managed via Dahu, an online server with upstream data reduction, data scaling and azimuthal integration built around PyFAI (Python Fast Azimuthal Integration), and data analysis performed using the open source FreeSAS. The results of this automated data analysis pipeline are displayed in ISPyB/ExiSAXS. The upgraded BM29 has been in operation since the post-EBS restart in September 2020, and here a full description of its new hardware and software characteristics together with examples of data obtained are provided. International Union of Crystallography 2023-01-01 /pmc/articles/PMC9814054/ /pubmed/36601945 http://dx.doi.org/10.1107/S1600577522011286 Text en © Mark D. Tully et al. 2023 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Beamlines Tully, Mark D. Kieffer, Jerome Brennich, Martha E. Cohen Aberdam, Raphael Florial, Jean Baptiste Hutin, Stephanie Oscarsson, Markus Beteva, Antonia Popov, Anton Moussaoui, Dihia Theveneau, Pascal Papp, Gergely Gigmes, Jonathan Cipriani, Florent McCarthy, Andrew Zubieta, Chloe Mueller-Dieckmann, Christoph Leonard, Gordon Pernot, Petra BioSAXS at European Synchrotron Radiation Facility – Extremely Brilliant Source: BM29 with an upgraded source, detector, robot, sample environment, data collection and analysis software |
title | BioSAXS at European Synchrotron Radiation Facility – Extremely Brilliant Source: BM29 with an upgraded source, detector, robot, sample environment, data collection and analysis software |
title_full | BioSAXS at European Synchrotron Radiation Facility – Extremely Brilliant Source: BM29 with an upgraded source, detector, robot, sample environment, data collection and analysis software |
title_fullStr | BioSAXS at European Synchrotron Radiation Facility – Extremely Brilliant Source: BM29 with an upgraded source, detector, robot, sample environment, data collection and analysis software |
title_full_unstemmed | BioSAXS at European Synchrotron Radiation Facility – Extremely Brilliant Source: BM29 with an upgraded source, detector, robot, sample environment, data collection and analysis software |
title_short | BioSAXS at European Synchrotron Radiation Facility – Extremely Brilliant Source: BM29 with an upgraded source, detector, robot, sample environment, data collection and analysis software |
title_sort | biosaxs at european synchrotron radiation facility – extremely brilliant source: bm29 with an upgraded source, detector, robot, sample environment, data collection and analysis software |
topic | Beamlines |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9814054/ https://www.ncbi.nlm.nih.gov/pubmed/36601945 http://dx.doi.org/10.1107/S1600577522011286 |
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