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Automated device for continuous stirring while sampling in liquid chromatography systems

Ultra-performance liquid chromatography is a common analysis tool, and stirring is common in many laboratory setups. Here we show a device which enables continuous stirring of samples whilst inside an ultra-performance liquid chromatography system. Utilizing standard magnetic stirring bars that fit...

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Autores principales: Markovitch, Omer, Ottelé, Jim, Veldman, Obe, Otto, Sijbren
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9814086/
https://www.ncbi.nlm.nih.gov/pubmed/36703458
http://dx.doi.org/10.1038/s42004-020-00427-5
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author Markovitch, Omer
Ottelé, Jim
Veldman, Obe
Otto, Sijbren
author_facet Markovitch, Omer
Ottelé, Jim
Veldman, Obe
Otto, Sijbren
author_sort Markovitch, Omer
collection PubMed
description Ultra-performance liquid chromatography is a common analysis tool, and stirring is common in many laboratory setups. Here we show a device which enables continuous stirring of samples whilst inside an ultra-performance liquid chromatography system. Utilizing standard magnetic stirring bars that fit standard vials, the device allows for the automation of experimental setups that require stirring. The device is designed such that it can replace the standard sample holder and fits in its place, while being battery operated. The use of three-dimensional (3D) printing and commercially available parts enables low-effort and low-cost device production, as well as easy modifications. Testing the device was performed by video analysis and by following the kinetics of a dynamic combinatorial library that is known to be exquisitely sensitive to agitation, as a result of involving a fiber growth-breakage mechanism. Design files and schematics are provided.
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spelling pubmed-98140862023-01-10 Automated device for continuous stirring while sampling in liquid chromatography systems Markovitch, Omer Ottelé, Jim Veldman, Obe Otto, Sijbren Commun Chem Article Ultra-performance liquid chromatography is a common analysis tool, and stirring is common in many laboratory setups. Here we show a device which enables continuous stirring of samples whilst inside an ultra-performance liquid chromatography system. Utilizing standard magnetic stirring bars that fit standard vials, the device allows for the automation of experimental setups that require stirring. The device is designed such that it can replace the standard sample holder and fits in its place, while being battery operated. The use of three-dimensional (3D) printing and commercially available parts enables low-effort and low-cost device production, as well as easy modifications. Testing the device was performed by video analysis and by following the kinetics of a dynamic combinatorial library that is known to be exquisitely sensitive to agitation, as a result of involving a fiber growth-breakage mechanism. Design files and schematics are provided. Nature Publishing Group UK 2020-12-03 /pmc/articles/PMC9814086/ /pubmed/36703458 http://dx.doi.org/10.1038/s42004-020-00427-5 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Markovitch, Omer
Ottelé, Jim
Veldman, Obe
Otto, Sijbren
Automated device for continuous stirring while sampling in liquid chromatography systems
title Automated device for continuous stirring while sampling in liquid chromatography systems
title_full Automated device for continuous stirring while sampling in liquid chromatography systems
title_fullStr Automated device for continuous stirring while sampling in liquid chromatography systems
title_full_unstemmed Automated device for continuous stirring while sampling in liquid chromatography systems
title_short Automated device for continuous stirring while sampling in liquid chromatography systems
title_sort automated device for continuous stirring while sampling in liquid chromatography systems
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9814086/
https://www.ncbi.nlm.nih.gov/pubmed/36703458
http://dx.doi.org/10.1038/s42004-020-00427-5
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