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MiRNAs from serum-derived extracellular vesicles as biomarkers for uveal melanoma progression

Uveal melanoma (UM) is a rare type of malignancy that originates from melanocytes in the choroid, iris and the eye’s ciliary body. Biomarkers for early detection and progression of UM, especially the molecular traits governing the development of metastasis, are still not available in clinical practi...

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Autores principales: Wróblewska, Joanna Patrycja, Lach, Michał Stefan, Rucinski, Marcin, Piotrowski, Igor, Galus, Lukasz, Suchorska, Wiktoria Maria, Kreis, Stephanie, Marszałek, Andrzej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9814164/
https://www.ncbi.nlm.nih.gov/pubmed/36619870
http://dx.doi.org/10.3389/fcell.2022.1008901
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author Wróblewska, Joanna Patrycja
Lach, Michał Stefan
Rucinski, Marcin
Piotrowski, Igor
Galus, Lukasz
Suchorska, Wiktoria Maria
Kreis, Stephanie
Marszałek, Andrzej
author_facet Wróblewska, Joanna Patrycja
Lach, Michał Stefan
Rucinski, Marcin
Piotrowski, Igor
Galus, Lukasz
Suchorska, Wiktoria Maria
Kreis, Stephanie
Marszałek, Andrzej
author_sort Wróblewska, Joanna Patrycja
collection PubMed
description Uveal melanoma (UM) is a rare type of malignancy that originates from melanocytes in the choroid, iris and the eye’s ciliary body. Biomarkers for early detection and progression of UM, especially the molecular traits governing the development of metastasis, are still not available in clinical practice. One extensively studied components of liquid biopsies are extracellular vesicles. Due to their unique molecular cargo, they can contribute to early cancer development and at the same time carry markers for disease onset and progression. For characterisation of the miRNA profiles present in circulating serum-derived exosomes of patients with diagnosed primary and metastatic UM, we have analyzed the miRNA cargos using next-generation sequencing followed by RT-qPCR validation in a cohort of patients (control n = 20; primary n = 9; metastatic n = 11). Nine miRNAs differentiating these patient groups have been established. We show that hsa-miR-144-5p and hsa-miR-191-5p are the most promising biomarker candidates, allowing the categorization of patients into local and advanced UM. Additionally, the comparison of miRNA expression levels in exosomes derived from UM patients with those derived from healthy donors revealed that hsa-miR-191-5p, -223-3p, -483-5p, -203a has the potential to be used as an early marker for the presence of UM. This pilot study reveals that miRNAs extracted from circulating exosomes could be exploited as potential biomarkers in UM diagnosis and, more importantly, for indicating metastatic spread.
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spelling pubmed-98141642023-01-06 MiRNAs from serum-derived extracellular vesicles as biomarkers for uveal melanoma progression Wróblewska, Joanna Patrycja Lach, Michał Stefan Rucinski, Marcin Piotrowski, Igor Galus, Lukasz Suchorska, Wiktoria Maria Kreis, Stephanie Marszałek, Andrzej Front Cell Dev Biol Cell and Developmental Biology Uveal melanoma (UM) is a rare type of malignancy that originates from melanocytes in the choroid, iris and the eye’s ciliary body. Biomarkers for early detection and progression of UM, especially the molecular traits governing the development of metastasis, are still not available in clinical practice. One extensively studied components of liquid biopsies are extracellular vesicles. Due to their unique molecular cargo, they can contribute to early cancer development and at the same time carry markers for disease onset and progression. For characterisation of the miRNA profiles present in circulating serum-derived exosomes of patients with diagnosed primary and metastatic UM, we have analyzed the miRNA cargos using next-generation sequencing followed by RT-qPCR validation in a cohort of patients (control n = 20; primary n = 9; metastatic n = 11). Nine miRNAs differentiating these patient groups have been established. We show that hsa-miR-144-5p and hsa-miR-191-5p are the most promising biomarker candidates, allowing the categorization of patients into local and advanced UM. Additionally, the comparison of miRNA expression levels in exosomes derived from UM patients with those derived from healthy donors revealed that hsa-miR-191-5p, -223-3p, -483-5p, -203a has the potential to be used as an early marker for the presence of UM. This pilot study reveals that miRNAs extracted from circulating exosomes could be exploited as potential biomarkers in UM diagnosis and, more importantly, for indicating metastatic spread. Frontiers Media S.A. 2022-12-22 /pmc/articles/PMC9814164/ /pubmed/36619870 http://dx.doi.org/10.3389/fcell.2022.1008901 Text en Copyright © 2022 Wróblewska, Lach, Rucinski, Piotrowski, Galus, Suchorska, Kreis and Marszałek. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Wróblewska, Joanna Patrycja
Lach, Michał Stefan
Rucinski, Marcin
Piotrowski, Igor
Galus, Lukasz
Suchorska, Wiktoria Maria
Kreis, Stephanie
Marszałek, Andrzej
MiRNAs from serum-derived extracellular vesicles as biomarkers for uveal melanoma progression
title MiRNAs from serum-derived extracellular vesicles as biomarkers for uveal melanoma progression
title_full MiRNAs from serum-derived extracellular vesicles as biomarkers for uveal melanoma progression
title_fullStr MiRNAs from serum-derived extracellular vesicles as biomarkers for uveal melanoma progression
title_full_unstemmed MiRNAs from serum-derived extracellular vesicles as biomarkers for uveal melanoma progression
title_short MiRNAs from serum-derived extracellular vesicles as biomarkers for uveal melanoma progression
title_sort mirnas from serum-derived extracellular vesicles as biomarkers for uveal melanoma progression
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9814164/
https://www.ncbi.nlm.nih.gov/pubmed/36619870
http://dx.doi.org/10.3389/fcell.2022.1008901
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