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Acyl-chain saturation regulates the order of phosphatidylinositol 4,5-bisphosphate nanodomains
Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P(2)) plays a critical role in the regulation of various plasma membrane processes and signaling pathways in eukaryotes. A significant amount of cellular resources are spent on maintaining the dominant 1-stearoyl-2-arachidonyl PI(4,5)P(2) acyl-chain comp...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9814227/ https://www.ncbi.nlm.nih.gov/pubmed/36697613 http://dx.doi.org/10.1038/s42004-021-00603-1 |
Sumario: | Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P(2)) plays a critical role in the regulation of various plasma membrane processes and signaling pathways in eukaryotes. A significant amount of cellular resources are spent on maintaining the dominant 1-stearoyl-2-arachidonyl PI(4,5)P(2) acyl-chain composition, while less abundant and more saturated species become more prevalent in response to specific stimuli, stress or aging. Here, we report the impact of acyl-chain structure on the biophysical properties of cation-induced PI(4,5)P(2) nanodomains. PI(4,5)P(2) species with increasing levels of acyl-chain saturation cluster in progressively more ordered nanodomains, culminating in the formation of gel-like nanodomains for fully saturated species. The formation of these gel-like domains was largely abrogated in the presence of 1-stearoyl-2-arachidonyl PI(4,5)P(2.) This is, to the best of our knowledge, the first report of the impact of PI(4,5)P(2) acyl-chain composition on cation-dependent nanodomain ordering, and provides important clues to the motives behind the enrichment of PI(4,5)P(2) with polyunsaturated acyl-chains. We also show how Ca(2+)-induced PI(4,5)P(2) nanodomains are able to generate local negative curvature, a phenomenon likely to play a role in membrane remodeling events. |
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