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Dual sequence definition increases the data storage capacity of sequence-defined macromolecules

Sequence-defined macromolecules offer applications in the field of data storage. Challenges include synthesising precise and pure sequences, reading stored information and increasing data storage capacity. Herein, the synthesis of dual sequence-defined oligomers and their application for data storag...

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Autores principales: Wetzel, Katharina S., Frölich, Maximiliane, Solleder, Susanne C., Nickisch, Roman, Treu, Philipp, Meier, Michael A. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9814518/
https://www.ncbi.nlm.nih.gov/pubmed/36703457
http://dx.doi.org/10.1038/s42004-020-0308-z
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author Wetzel, Katharina S.
Frölich, Maximiliane
Solleder, Susanne C.
Nickisch, Roman
Treu, Philipp
Meier, Michael A. R.
author_facet Wetzel, Katharina S.
Frölich, Maximiliane
Solleder, Susanne C.
Nickisch, Roman
Treu, Philipp
Meier, Michael A. R.
author_sort Wetzel, Katharina S.
collection PubMed
description Sequence-defined macromolecules offer applications in the field of data storage. Challenges include synthesising precise and pure sequences, reading stored information and increasing data storage capacity. Herein, the synthesis of dual sequence-defined oligomers and their application for data storage is demonstrated. While applying the well-established Passerini three-component reaction, the degree of definition of the prepared monodisperse macromolecules is improved compared to previous reports by utilising nine specifically designed isocyanide monomers to introduce backbone definition. The monomers are combined with various aldehyde components to synthesise dual-sequence defined oligomers. Thus, the side chains and the backbones of these macromolecules can be varied independently, exhibiting increased molecular diversity and hence data storage capacity per repeat unit. In case of a dual sequence-defined pentamer, 33 bits are achieved in a single molecule. The oligomers are obtained in multigram scale and excellent purity. Sequential read-out by tandem ESI-MS/MS verifies the high data storage capacity of the prepared oligomers per repeat unit in comparison to other sequence defined macromolecules.
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spelling pubmed-98145182023-01-10 Dual sequence definition increases the data storage capacity of sequence-defined macromolecules Wetzel, Katharina S. Frölich, Maximiliane Solleder, Susanne C. Nickisch, Roman Treu, Philipp Meier, Michael A. R. Commun Chem Article Sequence-defined macromolecules offer applications in the field of data storage. Challenges include synthesising precise and pure sequences, reading stored information and increasing data storage capacity. Herein, the synthesis of dual sequence-defined oligomers and their application for data storage is demonstrated. While applying the well-established Passerini three-component reaction, the degree of definition of the prepared monodisperse macromolecules is improved compared to previous reports by utilising nine specifically designed isocyanide monomers to introduce backbone definition. The monomers are combined with various aldehyde components to synthesise dual-sequence defined oligomers. Thus, the side chains and the backbones of these macromolecules can be varied independently, exhibiting increased molecular diversity and hence data storage capacity per repeat unit. In case of a dual sequence-defined pentamer, 33 bits are achieved in a single molecule. The oligomers are obtained in multigram scale and excellent purity. Sequential read-out by tandem ESI-MS/MS verifies the high data storage capacity of the prepared oligomers per repeat unit in comparison to other sequence defined macromolecules. Nature Publishing Group UK 2020-05-20 /pmc/articles/PMC9814518/ /pubmed/36703457 http://dx.doi.org/10.1038/s42004-020-0308-z Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wetzel, Katharina S.
Frölich, Maximiliane
Solleder, Susanne C.
Nickisch, Roman
Treu, Philipp
Meier, Michael A. R.
Dual sequence definition increases the data storage capacity of sequence-defined macromolecules
title Dual sequence definition increases the data storage capacity of sequence-defined macromolecules
title_full Dual sequence definition increases the data storage capacity of sequence-defined macromolecules
title_fullStr Dual sequence definition increases the data storage capacity of sequence-defined macromolecules
title_full_unstemmed Dual sequence definition increases the data storage capacity of sequence-defined macromolecules
title_short Dual sequence definition increases the data storage capacity of sequence-defined macromolecules
title_sort dual sequence definition increases the data storage capacity of sequence-defined macromolecules
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9814518/
https://www.ncbi.nlm.nih.gov/pubmed/36703457
http://dx.doi.org/10.1038/s42004-020-0308-z
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