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Streamlining of a synthetic co‐culture towards an individually controllable one‐pot process for polyhydroxyalkanoate production from light and CO(2)

Rationally designed synthetic microbial consortia carry a vast potential for biotechnological applications. The application of such a consortium in a bioprocess, however, requires tight and individual controllability of the involved microbes. Here, we present the streamlining of a co‐cultivation pro...

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Autores principales: Kratzl, Franziska, Kremling, Andreas, Pflüger‐Grau, Katharina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9815089/
https://www.ncbi.nlm.nih.gov/pubmed/36619884
http://dx.doi.org/10.1002/elsc.202100156
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author Kratzl, Franziska
Kremling, Andreas
Pflüger‐Grau, Katharina
author_facet Kratzl, Franziska
Kremling, Andreas
Pflüger‐Grau, Katharina
author_sort Kratzl, Franziska
collection PubMed
description Rationally designed synthetic microbial consortia carry a vast potential for biotechnological applications. The application of such a consortium in a bioprocess, however, requires tight and individual controllability of the involved microbes. Here, we present the streamlining of a co‐cultivation process consisting of Synechococcus elongatus cscB and Pseudomonas putida for the production of polyhydroxyalkanoates (PHA) from light and CO(2). First, the process was improved by employing P. putida cscRABY, a strain with a higher metabolic activity towards sucrose. Next, the individual controllability of the co‐culture partners was addressed by providing different nitrogen sources, each exclusively available for one strain. By this, the growth rate of the co‐culture partners could be regulated individually, and defined conditions could be set. The molC/molN ratio, a key value for PHA accumulation, was estimated from the experimental data, and the necessary feeding rates to obtain a specific ratio could be predicted. This information was then implemented in the co‐cultivation process, following the concept of a DBTL‐cycle. In total, the streamlining of the process resulted in an increased maximal PHA titer of 393 mg/L and a PHA production rate of 42.1 mg/(L•day).
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spelling pubmed-98150892023-01-05 Streamlining of a synthetic co‐culture towards an individually controllable one‐pot process for polyhydroxyalkanoate production from light and CO(2) Kratzl, Franziska Kremling, Andreas Pflüger‐Grau, Katharina Eng Life Sci Research Article Rationally designed synthetic microbial consortia carry a vast potential for biotechnological applications. The application of such a consortium in a bioprocess, however, requires tight and individual controllability of the involved microbes. Here, we present the streamlining of a co‐cultivation process consisting of Synechococcus elongatus cscB and Pseudomonas putida for the production of polyhydroxyalkanoates (PHA) from light and CO(2). First, the process was improved by employing P. putida cscRABY, a strain with a higher metabolic activity towards sucrose. Next, the individual controllability of the co‐culture partners was addressed by providing different nitrogen sources, each exclusively available for one strain. By this, the growth rate of the co‐culture partners could be regulated individually, and defined conditions could be set. The molC/molN ratio, a key value for PHA accumulation, was estimated from the experimental data, and the necessary feeding rates to obtain a specific ratio could be predicted. This information was then implemented in the co‐cultivation process, following the concept of a DBTL‐cycle. In total, the streamlining of the process resulted in an increased maximal PHA titer of 393 mg/L and a PHA production rate of 42.1 mg/(L•day). John Wiley and Sons Inc. 2022-02-15 /pmc/articles/PMC9815089/ /pubmed/36619884 http://dx.doi.org/10.1002/elsc.202100156 Text en © 2022 The Authors. Engineering in Life Sciences published by Wiley‐VCH GmbH. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kratzl, Franziska
Kremling, Andreas
Pflüger‐Grau, Katharina
Streamlining of a synthetic co‐culture towards an individually controllable one‐pot process for polyhydroxyalkanoate production from light and CO(2)
title Streamlining of a synthetic co‐culture towards an individually controllable one‐pot process for polyhydroxyalkanoate production from light and CO(2)
title_full Streamlining of a synthetic co‐culture towards an individually controllable one‐pot process for polyhydroxyalkanoate production from light and CO(2)
title_fullStr Streamlining of a synthetic co‐culture towards an individually controllable one‐pot process for polyhydroxyalkanoate production from light and CO(2)
title_full_unstemmed Streamlining of a synthetic co‐culture towards an individually controllable one‐pot process for polyhydroxyalkanoate production from light and CO(2)
title_short Streamlining of a synthetic co‐culture towards an individually controllable one‐pot process for polyhydroxyalkanoate production from light and CO(2)
title_sort streamlining of a synthetic co‐culture towards an individually controllable one‐pot process for polyhydroxyalkanoate production from light and co(2)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9815089/
https://www.ncbi.nlm.nih.gov/pubmed/36619884
http://dx.doi.org/10.1002/elsc.202100156
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