Cargando…

Detection of ryanodine receptor G4911E and I4754M mutation sites and analysis of binding modes of diamide insecticides with RyR on Galeruca daurica (Coleoptera: Chrysomelidae)

In recent years, the leaf beetle Galeruca daurica has broken out in the northern grasslands of Inner Mongolia, its management still mainly depends on chemical control using traditional insecticides or with novel action. The study was aim to identify mutation locus associated with resistance to diami...

Descripción completa

Detalles Bibliográficos
Autores principales: Ren, Hao, Zhang, Hongling, Ni, Ruoyao, Li, Yanyan, Li, Ling, Wang, Wenhe, Tian, Yu, Pang, Baoping, Tan, Yao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9815114/
https://www.ncbi.nlm.nih.gov/pubmed/36620218
http://dx.doi.org/10.3389/fphys.2022.1107045
_version_ 1784864283508604928
author Ren, Hao
Zhang, Hongling
Ni, Ruoyao
Li, Yanyan
Li, Ling
Wang, Wenhe
Tian, Yu
Pang, Baoping
Tan, Yao
author_facet Ren, Hao
Zhang, Hongling
Ni, Ruoyao
Li, Yanyan
Li, Ling
Wang, Wenhe
Tian, Yu
Pang, Baoping
Tan, Yao
author_sort Ren, Hao
collection PubMed
description In recent years, the leaf beetle Galeruca daurica has broken out in the northern grasslands of Inner Mongolia, its management still mainly depends on chemical control using traditional insecticides or with novel action. The study was aim to identify mutation locus associated with resistance to diamide insecticides in field population of G. daurica, to provide a reference for rational selection of insecticides and to avoid the rapid resistance development to diamide insecticides. We cloned the full length of the ryanodine receptor gene of G. daurica (GdRyR), constructed 3D model and transmembrane regions by homologous modeling based on deduced amino acid sequence. Two potential mutation loci (Gly4911Glu and Ile4754Met) and allelic mutation frequencies were detected in individuals of G. daurica. In addition, their binding patterns to two diamide insecticides (chlorantraniliprole, cyantraniliprole) were analyzed separately using a molecular docking method. The full-length cDNA sequence of GdRyR (GenBank accession number: OP828593) was obtained by splicing and assembling, which is 15,399 bp in length and encodes 5,133 amino acids. The amino acid similarity of GdRyR with that of other Coleopteran insects were 86.70%–91.33%, which possessed the typical structural characteristics. An individual resistance allelic mutation frequency test on fifty field leaf beetles has identified 12% and 32% heterozygous individuals at two potential mutation loci Gly4911Glu and Ile4754Met, respectively. The affinity of the I4754M mutant model of GdRyR for chlorantraniliprole and cyantraniliprole was not significantly different from that of the wild type, and all had non-covalent interactions such as hydrogen bonding, hydrophobic interactions and π-cation interactions. However, the G4911E mutant model showed reduced affinity and reduced mode of action with two diamide insecticides, thus affecting the binding stability of the ryanodine receptor to the diamide insecticides. In conclusion, the G4911E mutation in GdRyR may be a potential mechanism for the development of resistance to diamide insecticides on G. daurica and should be a key concern for resistance risk assessment and reasonable applications of diamide insecticides for control in future. Moreover, this study could provide a reference for ryanodine receptor structure-based insecticides design.
format Online
Article
Text
id pubmed-9815114
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-98151142023-01-06 Detection of ryanodine receptor G4911E and I4754M mutation sites and analysis of binding modes of diamide insecticides with RyR on Galeruca daurica (Coleoptera: Chrysomelidae) Ren, Hao Zhang, Hongling Ni, Ruoyao Li, Yanyan Li, Ling Wang, Wenhe Tian, Yu Pang, Baoping Tan, Yao Front Physiol Physiology In recent years, the leaf beetle Galeruca daurica has broken out in the northern grasslands of Inner Mongolia, its management still mainly depends on chemical control using traditional insecticides or with novel action. The study was aim to identify mutation locus associated with resistance to diamide insecticides in field population of G. daurica, to provide a reference for rational selection of insecticides and to avoid the rapid resistance development to diamide insecticides. We cloned the full length of the ryanodine receptor gene of G. daurica (GdRyR), constructed 3D model and transmembrane regions by homologous modeling based on deduced amino acid sequence. Two potential mutation loci (Gly4911Glu and Ile4754Met) and allelic mutation frequencies were detected in individuals of G. daurica. In addition, their binding patterns to two diamide insecticides (chlorantraniliprole, cyantraniliprole) were analyzed separately using a molecular docking method. The full-length cDNA sequence of GdRyR (GenBank accession number: OP828593) was obtained by splicing and assembling, which is 15,399 bp in length and encodes 5,133 amino acids. The amino acid similarity of GdRyR with that of other Coleopteran insects were 86.70%–91.33%, which possessed the typical structural characteristics. An individual resistance allelic mutation frequency test on fifty field leaf beetles has identified 12% and 32% heterozygous individuals at two potential mutation loci Gly4911Glu and Ile4754Met, respectively. The affinity of the I4754M mutant model of GdRyR for chlorantraniliprole and cyantraniliprole was not significantly different from that of the wild type, and all had non-covalent interactions such as hydrogen bonding, hydrophobic interactions and π-cation interactions. However, the G4911E mutant model showed reduced affinity and reduced mode of action with two diamide insecticides, thus affecting the binding stability of the ryanodine receptor to the diamide insecticides. In conclusion, the G4911E mutation in GdRyR may be a potential mechanism for the development of resistance to diamide insecticides on G. daurica and should be a key concern for resistance risk assessment and reasonable applications of diamide insecticides for control in future. Moreover, this study could provide a reference for ryanodine receptor structure-based insecticides design. Frontiers Media S.A. 2022-12-22 /pmc/articles/PMC9815114/ /pubmed/36620218 http://dx.doi.org/10.3389/fphys.2022.1107045 Text en Copyright © 2022 Ren, Zhang, Ni, Li, Li, Wang, Tian, Pang and Tan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Ren, Hao
Zhang, Hongling
Ni, Ruoyao
Li, Yanyan
Li, Ling
Wang, Wenhe
Tian, Yu
Pang, Baoping
Tan, Yao
Detection of ryanodine receptor G4911E and I4754M mutation sites and analysis of binding modes of diamide insecticides with RyR on Galeruca daurica (Coleoptera: Chrysomelidae)
title Detection of ryanodine receptor G4911E and I4754M mutation sites and analysis of binding modes of diamide insecticides with RyR on Galeruca daurica (Coleoptera: Chrysomelidae)
title_full Detection of ryanodine receptor G4911E and I4754M mutation sites and analysis of binding modes of diamide insecticides with RyR on Galeruca daurica (Coleoptera: Chrysomelidae)
title_fullStr Detection of ryanodine receptor G4911E and I4754M mutation sites and analysis of binding modes of diamide insecticides with RyR on Galeruca daurica (Coleoptera: Chrysomelidae)
title_full_unstemmed Detection of ryanodine receptor G4911E and I4754M mutation sites and analysis of binding modes of diamide insecticides with RyR on Galeruca daurica (Coleoptera: Chrysomelidae)
title_short Detection of ryanodine receptor G4911E and I4754M mutation sites and analysis of binding modes of diamide insecticides with RyR on Galeruca daurica (Coleoptera: Chrysomelidae)
title_sort detection of ryanodine receptor g4911e and i4754m mutation sites and analysis of binding modes of diamide insecticides with ryr on galeruca daurica (coleoptera: chrysomelidae)
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9815114/
https://www.ncbi.nlm.nih.gov/pubmed/36620218
http://dx.doi.org/10.3389/fphys.2022.1107045
work_keys_str_mv AT renhao detectionofryanodinereceptorg4911eandi4754mmutationsitesandanalysisofbindingmodesofdiamideinsecticideswithryrongalerucadauricacoleopterachrysomelidae
AT zhanghongling detectionofryanodinereceptorg4911eandi4754mmutationsitesandanalysisofbindingmodesofdiamideinsecticideswithryrongalerucadauricacoleopterachrysomelidae
AT niruoyao detectionofryanodinereceptorg4911eandi4754mmutationsitesandanalysisofbindingmodesofdiamideinsecticideswithryrongalerucadauricacoleopterachrysomelidae
AT liyanyan detectionofryanodinereceptorg4911eandi4754mmutationsitesandanalysisofbindingmodesofdiamideinsecticideswithryrongalerucadauricacoleopterachrysomelidae
AT liling detectionofryanodinereceptorg4911eandi4754mmutationsitesandanalysisofbindingmodesofdiamideinsecticideswithryrongalerucadauricacoleopterachrysomelidae
AT wangwenhe detectionofryanodinereceptorg4911eandi4754mmutationsitesandanalysisofbindingmodesofdiamideinsecticideswithryrongalerucadauricacoleopterachrysomelidae
AT tianyu detectionofryanodinereceptorg4911eandi4754mmutationsitesandanalysisofbindingmodesofdiamideinsecticideswithryrongalerucadauricacoleopterachrysomelidae
AT pangbaoping detectionofryanodinereceptorg4911eandi4754mmutationsitesandanalysisofbindingmodesofdiamideinsecticideswithryrongalerucadauricacoleopterachrysomelidae
AT tanyao detectionofryanodinereceptorg4911eandi4754mmutationsitesandanalysisofbindingmodesofdiamideinsecticideswithryrongalerucadauricacoleopterachrysomelidae