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Girdin acts as an oncogene in gastric cancer by regulating AKT/GSK3β/β-catenin signaling

ThE present work focused on exploring Girdin expression within gastric cancer (GC), examining the effect of Girdin on the cell phenotype of GC, and clarifying the underlying mechanisms. Girdin expression in GC samples was identified by immunohistochemistry (IHC) and quantitative real-time PCR (qRT-P...

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Autores principales: Wang, Yun, Fu, Qiang, Tao, Yun-jian, Ying, Sheng-nan, Zhong, Heng-gao, Zhu, Yue, Qian, Xiao-han, Miao, Lin, Yang, Li-hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9816263/
https://www.ncbi.nlm.nih.gov/pubmed/36604355
http://dx.doi.org/10.1007/s10142-022-00927-8
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author Wang, Yun
Fu, Qiang
Tao, Yun-jian
Ying, Sheng-nan
Zhong, Heng-gao
Zhu, Yue
Qian, Xiao-han
Miao, Lin
Yang, Li-hua
author_facet Wang, Yun
Fu, Qiang
Tao, Yun-jian
Ying, Sheng-nan
Zhong, Heng-gao
Zhu, Yue
Qian, Xiao-han
Miao, Lin
Yang, Li-hua
author_sort Wang, Yun
collection PubMed
description ThE present work focused on exploring Girdin expression within gastric cancer (GC), examining the effect of Girdin on the cell phenotype of GC, and clarifying the underlying mechanisms. Girdin expression in GC samples was identified by immunohistochemistry (IHC) and quantitative real-time PCR (qRT-PCR) assays. Girdin-targeting siRNAs were transfected into GC cells; later, we examined GC cell proliferation, migration, invasion, and apoptosis, respectively. Additionally, the protein expression was examined through Western blotting assay. Moreover, the tumor implantation experiment was conducted for examining Girdin knockdown in vivo. The results showed that Girdin expression elevated within GC samples, which was associated with the dismal prognostic outcome. Girdin knockdown suppressed GC cell proliferation, migration, and invasion, and enhanced apoptosis and cell cycle arrest. Girdin promoted the phosphorylation of AKT, GSK3β, and β-catenin. Moreover, Girdin inhibited the phosphorylation of β-catenin. Girdin suppressed cell apoptosis and stimulated cell migration and invasion, while AKT inhibitor (MK2206) treatment reversed the effect of Girdin overexpression, and GSK3β inhibitor (CHIR99021) treatment enhanced the effect of Girdin overexpression on GC cells. Besides, Girdin delayed tumor growth in vivo. In conclusion, Girdin was abnormally expressed in GC samples, which promoted the development of GC by regulating AKT/GSK3β/β-catenin signaling.
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spelling pubmed-98162632023-01-07 Girdin acts as an oncogene in gastric cancer by regulating AKT/GSK3β/β-catenin signaling Wang, Yun Fu, Qiang Tao, Yun-jian Ying, Sheng-nan Zhong, Heng-gao Zhu, Yue Qian, Xiao-han Miao, Lin Yang, Li-hua Funct Integr Genomics Original Article ThE present work focused on exploring Girdin expression within gastric cancer (GC), examining the effect of Girdin on the cell phenotype of GC, and clarifying the underlying mechanisms. Girdin expression in GC samples was identified by immunohistochemistry (IHC) and quantitative real-time PCR (qRT-PCR) assays. Girdin-targeting siRNAs were transfected into GC cells; later, we examined GC cell proliferation, migration, invasion, and apoptosis, respectively. Additionally, the protein expression was examined through Western blotting assay. Moreover, the tumor implantation experiment was conducted for examining Girdin knockdown in vivo. The results showed that Girdin expression elevated within GC samples, which was associated with the dismal prognostic outcome. Girdin knockdown suppressed GC cell proliferation, migration, and invasion, and enhanced apoptosis and cell cycle arrest. Girdin promoted the phosphorylation of AKT, GSK3β, and β-catenin. Moreover, Girdin inhibited the phosphorylation of β-catenin. Girdin suppressed cell apoptosis and stimulated cell migration and invasion, while AKT inhibitor (MK2206) treatment reversed the effect of Girdin overexpression, and GSK3β inhibitor (CHIR99021) treatment enhanced the effect of Girdin overexpression on GC cells. Besides, Girdin delayed tumor growth in vivo. In conclusion, Girdin was abnormally expressed in GC samples, which promoted the development of GC by regulating AKT/GSK3β/β-catenin signaling. Springer Berlin Heidelberg 2023-01-06 2023 /pmc/articles/PMC9816263/ /pubmed/36604355 http://dx.doi.org/10.1007/s10142-022-00927-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Wang, Yun
Fu, Qiang
Tao, Yun-jian
Ying, Sheng-nan
Zhong, Heng-gao
Zhu, Yue
Qian, Xiao-han
Miao, Lin
Yang, Li-hua
Girdin acts as an oncogene in gastric cancer by regulating AKT/GSK3β/β-catenin signaling
title Girdin acts as an oncogene in gastric cancer by regulating AKT/GSK3β/β-catenin signaling
title_full Girdin acts as an oncogene in gastric cancer by regulating AKT/GSK3β/β-catenin signaling
title_fullStr Girdin acts as an oncogene in gastric cancer by regulating AKT/GSK3β/β-catenin signaling
title_full_unstemmed Girdin acts as an oncogene in gastric cancer by regulating AKT/GSK3β/β-catenin signaling
title_short Girdin acts as an oncogene in gastric cancer by regulating AKT/GSK3β/β-catenin signaling
title_sort girdin acts as an oncogene in gastric cancer by regulating akt/gsk3β/β-catenin signaling
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9816263/
https://www.ncbi.nlm.nih.gov/pubmed/36604355
http://dx.doi.org/10.1007/s10142-022-00927-8
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