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The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth
INTRODUCTION: To survive in various hostile environments, two-component system is an adaptive mechanism for diverse bacteria. Activity of the CpxA/CpxR two-component system contributes to coping with different stimuli, such as pH, osmotic and heat stress. METHODS: However, the role of the CpxA/CpxR...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9816388/ https://www.ncbi.nlm.nih.gov/pubmed/36619992 http://dx.doi.org/10.3389/fmicb.2022.1079390 |
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author | Yao, Qing Xie, Tingting Fu, Yu Wan, Jiajia Zhang, Wendie Gao, Xuejun Huang, Jing Sun, Diangang Zhang, Fuxian Bei, Weicheng Lei, Liancheng Liu, Feng |
author_facet | Yao, Qing Xie, Tingting Fu, Yu Wan, Jiajia Zhang, Wendie Gao, Xuejun Huang, Jing Sun, Diangang Zhang, Fuxian Bei, Weicheng Lei, Liancheng Liu, Feng |
author_sort | Yao, Qing |
collection | PubMed |
description | INTRODUCTION: To survive in various hostile environments, two-component system is an adaptive mechanism for diverse bacteria. Activity of the CpxA/CpxR two-component system contributes to coping with different stimuli, such as pH, osmotic and heat stress. METHODS: However, the role of the CpxA/CpxR system in cold resistance is little-known. In this study, we showed that CpxA/CpxRwas critical for A. pleuropneumoniae growth under cold stress. RESULTS: β-Galactosidaseanalysis showed that CpxA/CpxR positively regulated the predicted cold stress gene cspC. The mutant for cold stress gene cspC was impaired in the optimal growth of A. pleuropneumoniae under cold stress. Furthermore, electrophoretic mobility shift assays demonstrated that CpxR-P could directly regulate the transcription of the cold stress gene cspC. DISCUSSION: These results presented in this study illustrated that the CpxA/CpxR system plays an important role in cold resistance by upregulating expression of CspC. The data give new insights into how A. pleuropneumoniae survives in cold stress. |
format | Online Article Text |
id | pubmed-9816388 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98163882023-01-07 The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth Yao, Qing Xie, Tingting Fu, Yu Wan, Jiajia Zhang, Wendie Gao, Xuejun Huang, Jing Sun, Diangang Zhang, Fuxian Bei, Weicheng Lei, Liancheng Liu, Feng Front Microbiol Microbiology INTRODUCTION: To survive in various hostile environments, two-component system is an adaptive mechanism for diverse bacteria. Activity of the CpxA/CpxR two-component system contributes to coping with different stimuli, such as pH, osmotic and heat stress. METHODS: However, the role of the CpxA/CpxR system in cold resistance is little-known. In this study, we showed that CpxA/CpxRwas critical for A. pleuropneumoniae growth under cold stress. RESULTS: β-Galactosidaseanalysis showed that CpxA/CpxR positively regulated the predicted cold stress gene cspC. The mutant for cold stress gene cspC was impaired in the optimal growth of A. pleuropneumoniae under cold stress. Furthermore, electrophoretic mobility shift assays demonstrated that CpxR-P could directly regulate the transcription of the cold stress gene cspC. DISCUSSION: These results presented in this study illustrated that the CpxA/CpxR system plays an important role in cold resistance by upregulating expression of CspC. The data give new insights into how A. pleuropneumoniae survives in cold stress. Frontiers Media S.A. 2022-12-23 /pmc/articles/PMC9816388/ /pubmed/36619992 http://dx.doi.org/10.3389/fmicb.2022.1079390 Text en Copyright © 2022 Yao, Xie, Fu, Wan, Zhang, Gao, Huang, Sun, Zhang, Bei, Lei and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Yao, Qing Xie, Tingting Fu, Yu Wan, Jiajia Zhang, Wendie Gao, Xuejun Huang, Jing Sun, Diangang Zhang, Fuxian Bei, Weicheng Lei, Liancheng Liu, Feng The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth |
title | The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth |
title_full | The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth |
title_fullStr | The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth |
title_full_unstemmed | The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth |
title_short | The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth |
title_sort | cpxa/cpxr two-component system mediates regulation of actinobacillus pleuropneumoniae cold growth |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9816388/ https://www.ncbi.nlm.nih.gov/pubmed/36619992 http://dx.doi.org/10.3389/fmicb.2022.1079390 |
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