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The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth

INTRODUCTION: To survive in various hostile environments, two-component system is an adaptive mechanism for diverse bacteria. Activity of the CpxA/CpxR two-component system contributes to coping with different stimuli, such as pH, osmotic and heat stress. METHODS: However, the role of the CpxA/CpxR...

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Autores principales: Yao, Qing, Xie, Tingting, Fu, Yu, Wan, Jiajia, Zhang, Wendie, Gao, Xuejun, Huang, Jing, Sun, Diangang, Zhang, Fuxian, Bei, Weicheng, Lei, Liancheng, Liu, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9816388/
https://www.ncbi.nlm.nih.gov/pubmed/36619992
http://dx.doi.org/10.3389/fmicb.2022.1079390
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author Yao, Qing
Xie, Tingting
Fu, Yu
Wan, Jiajia
Zhang, Wendie
Gao, Xuejun
Huang, Jing
Sun, Diangang
Zhang, Fuxian
Bei, Weicheng
Lei, Liancheng
Liu, Feng
author_facet Yao, Qing
Xie, Tingting
Fu, Yu
Wan, Jiajia
Zhang, Wendie
Gao, Xuejun
Huang, Jing
Sun, Diangang
Zhang, Fuxian
Bei, Weicheng
Lei, Liancheng
Liu, Feng
author_sort Yao, Qing
collection PubMed
description INTRODUCTION: To survive in various hostile environments, two-component system is an adaptive mechanism for diverse bacteria. Activity of the CpxA/CpxR two-component system contributes to coping with different stimuli, such as pH, osmotic and heat stress. METHODS: However, the role of the CpxA/CpxR system in cold resistance is little-known. In this study, we showed that CpxA/CpxRwas critical for A. pleuropneumoniae growth under cold stress. RESULTS: β-Galactosidaseanalysis showed that CpxA/CpxR positively regulated the predicted cold stress gene cspC. The mutant for cold stress gene cspC was impaired in the optimal growth of A. pleuropneumoniae under cold stress. Furthermore, electrophoretic mobility shift assays demonstrated that CpxR-P could directly regulate the transcription of the cold stress gene cspC. DISCUSSION: These results presented in this study illustrated that the CpxA/CpxR system plays an important role in cold resistance by upregulating expression of CspC. The data give new insights into how A. pleuropneumoniae survives in cold stress.
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spelling pubmed-98163882023-01-07 The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth Yao, Qing Xie, Tingting Fu, Yu Wan, Jiajia Zhang, Wendie Gao, Xuejun Huang, Jing Sun, Diangang Zhang, Fuxian Bei, Weicheng Lei, Liancheng Liu, Feng Front Microbiol Microbiology INTRODUCTION: To survive in various hostile environments, two-component system is an adaptive mechanism for diverse bacteria. Activity of the CpxA/CpxR two-component system contributes to coping with different stimuli, such as pH, osmotic and heat stress. METHODS: However, the role of the CpxA/CpxR system in cold resistance is little-known. In this study, we showed that CpxA/CpxRwas critical for A. pleuropneumoniae growth under cold stress. RESULTS: β-Galactosidaseanalysis showed that CpxA/CpxR positively regulated the predicted cold stress gene cspC. The mutant for cold stress gene cspC was impaired in the optimal growth of A. pleuropneumoniae under cold stress. Furthermore, electrophoretic mobility shift assays demonstrated that CpxR-P could directly regulate the transcription of the cold stress gene cspC. DISCUSSION: These results presented in this study illustrated that the CpxA/CpxR system plays an important role in cold resistance by upregulating expression of CspC. The data give new insights into how A. pleuropneumoniae survives in cold stress. Frontiers Media S.A. 2022-12-23 /pmc/articles/PMC9816388/ /pubmed/36619992 http://dx.doi.org/10.3389/fmicb.2022.1079390 Text en Copyright © 2022 Yao, Xie, Fu, Wan, Zhang, Gao, Huang, Sun, Zhang, Bei, Lei and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yao, Qing
Xie, Tingting
Fu, Yu
Wan, Jiajia
Zhang, Wendie
Gao, Xuejun
Huang, Jing
Sun, Diangang
Zhang, Fuxian
Bei, Weicheng
Lei, Liancheng
Liu, Feng
The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth
title The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth
title_full The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth
title_fullStr The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth
title_full_unstemmed The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth
title_short The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth
title_sort cpxa/cpxr two-component system mediates regulation of actinobacillus pleuropneumoniae cold growth
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9816388/
https://www.ncbi.nlm.nih.gov/pubmed/36619992
http://dx.doi.org/10.3389/fmicb.2022.1079390
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