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Comparative analysis of fungal communities between herbicide-resistant and -susceptible Alopecurus aequalis

INTRODUCTION: Alopecurus aequalis is a grass species invading Chinese canola and wheat fields. An A. aequalis KMN-R population surviving mesosulfuron-methyl treatment with recommended rates was acquired from wheatland. Here, we aimed to confirm the resistance profiles of KMN-R to acetolactate synthe...

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Autores principales: Zhan, You, Liu, Haozhe, Cao, Ziheng, Chen, Wen, Li, Zongfang, Bai, Lianyang, Pan, Lang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9816403/
https://www.ncbi.nlm.nih.gov/pubmed/36619755
http://dx.doi.org/10.3389/fcimb.2022.1094853
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author Zhan, You
Liu, Haozhe
Cao, Ziheng
Chen, Wen
Li, Zongfang
Bai, Lianyang
Pan, Lang
author_facet Zhan, You
Liu, Haozhe
Cao, Ziheng
Chen, Wen
Li, Zongfang
Bai, Lianyang
Pan, Lang
author_sort Zhan, You
collection PubMed
description INTRODUCTION: Alopecurus aequalis is a grass species invading Chinese canola and wheat fields. An A. aequalis KMN-R population surviving mesosulfuron-methyl treatment with recommended rates was acquired from wheatland. Here, we aimed to confirm the resistance profiles of KMN-R to acetolactate synthetase (ALS) inhibiting herbicides and explore the possible resistance mechanisms to mesosulfuron-methyl in this weed population. METHODS: The dose-response tests performed in our study were used to test the toxicity of A. aequalis to ALS-inhibiting herbicides. Sanger sequencing was used to analyze the ALS gene of mesosulfuron-methyl -resistant and -susceptible A. aequalis. RNA sequencing analysis was used to find candidate genes that may confer metabolic resistance to the mesosulfuron-methyl in resistant A. aequalis population. Mesosulfuron-methyl -resistant and -susceptible A. aequalis populations fungal composition was measured via Illumina MiSeq Sequencing. RESULTS: Dose-response results indicated that KMN-R population evolved resistance to mesosulfuron-methyl and other tested ALS-inhibiting herbicides. Known resistance-conferring Trp-574-Leu gene mutation in A. aequalis ALS was detected in the KMN-R population. Pretreatment with 4-chloro-7-nitrobenzoxadiazole reversed mesosulfuron-methyl resistance in KMN-R. Glutathione S-transferases (GST) gene GSTZ2 and GSTT3 were highly expressed in KMN-R population. In addition, we evaluated the alpha diversity in A. aequalis, centering on OTU abundance, equality, and multiplicity, and found that the fungal community composition had more unexplained variance between KMN-R and KMN-S A. aequalis. We also observed higher abundances of specific fungi in KMN-R A. aequalis. DISCUSSION: The results proved that resistance to mesosulfuron-methyl in A. aequalis KMN-R population is probably caused by target site- and non-target site-based relating GST and provided the basis for further research between fungal interaction and herbicide resistance.
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spelling pubmed-98164032023-01-07 Comparative analysis of fungal communities between herbicide-resistant and -susceptible Alopecurus aequalis Zhan, You Liu, Haozhe Cao, Ziheng Chen, Wen Li, Zongfang Bai, Lianyang Pan, Lang Front Cell Infect Microbiol Cellular and Infection Microbiology INTRODUCTION: Alopecurus aequalis is a grass species invading Chinese canola and wheat fields. An A. aequalis KMN-R population surviving mesosulfuron-methyl treatment with recommended rates was acquired from wheatland. Here, we aimed to confirm the resistance profiles of KMN-R to acetolactate synthetase (ALS) inhibiting herbicides and explore the possible resistance mechanisms to mesosulfuron-methyl in this weed population. METHODS: The dose-response tests performed in our study were used to test the toxicity of A. aequalis to ALS-inhibiting herbicides. Sanger sequencing was used to analyze the ALS gene of mesosulfuron-methyl -resistant and -susceptible A. aequalis. RNA sequencing analysis was used to find candidate genes that may confer metabolic resistance to the mesosulfuron-methyl in resistant A. aequalis population. Mesosulfuron-methyl -resistant and -susceptible A. aequalis populations fungal composition was measured via Illumina MiSeq Sequencing. RESULTS: Dose-response results indicated that KMN-R population evolved resistance to mesosulfuron-methyl and other tested ALS-inhibiting herbicides. Known resistance-conferring Trp-574-Leu gene mutation in A. aequalis ALS was detected in the KMN-R population. Pretreatment with 4-chloro-7-nitrobenzoxadiazole reversed mesosulfuron-methyl resistance in KMN-R. Glutathione S-transferases (GST) gene GSTZ2 and GSTT3 were highly expressed in KMN-R population. In addition, we evaluated the alpha diversity in A. aequalis, centering on OTU abundance, equality, and multiplicity, and found that the fungal community composition had more unexplained variance between KMN-R and KMN-S A. aequalis. We also observed higher abundances of specific fungi in KMN-R A. aequalis. DISCUSSION: The results proved that resistance to mesosulfuron-methyl in A. aequalis KMN-R population is probably caused by target site- and non-target site-based relating GST and provided the basis for further research between fungal interaction and herbicide resistance. Frontiers Media S.A. 2022-12-23 /pmc/articles/PMC9816403/ /pubmed/36619755 http://dx.doi.org/10.3389/fcimb.2022.1094853 Text en Copyright © 2022 Zhan, Liu, Cao, Chen, Li, Bai and Pan https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Zhan, You
Liu, Haozhe
Cao, Ziheng
Chen, Wen
Li, Zongfang
Bai, Lianyang
Pan, Lang
Comparative analysis of fungal communities between herbicide-resistant and -susceptible Alopecurus aequalis
title Comparative analysis of fungal communities between herbicide-resistant and -susceptible Alopecurus aequalis
title_full Comparative analysis of fungal communities between herbicide-resistant and -susceptible Alopecurus aequalis
title_fullStr Comparative analysis of fungal communities between herbicide-resistant and -susceptible Alopecurus aequalis
title_full_unstemmed Comparative analysis of fungal communities between herbicide-resistant and -susceptible Alopecurus aequalis
title_short Comparative analysis of fungal communities between herbicide-resistant and -susceptible Alopecurus aequalis
title_sort comparative analysis of fungal communities between herbicide-resistant and -susceptible alopecurus aequalis
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9816403/
https://www.ncbi.nlm.nih.gov/pubmed/36619755
http://dx.doi.org/10.3389/fcimb.2022.1094853
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