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Methods to mechanically perturb and characterize GUV-based minimal cell models

Cells shield organelles and the cytosol via an active boundary predominantly made of phospholipids and membrane proteins, yet allowing communication between the intracellular and extracellular environment. Micron-sized liposome compartments commonly known as giant unilamellar vesicles (GUVs) are use...

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Detalles Bibliográficos
Autores principales: Wubshet, Nadab H., Liu, Allen P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research Network of Computational and Structural Biotechnology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9816913/
https://www.ncbi.nlm.nih.gov/pubmed/36659916
http://dx.doi.org/10.1016/j.csbj.2022.12.025
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author Wubshet, Nadab H.
Liu, Allen P.
author_facet Wubshet, Nadab H.
Liu, Allen P.
author_sort Wubshet, Nadab H.
collection PubMed
description Cells shield organelles and the cytosol via an active boundary predominantly made of phospholipids and membrane proteins, yet allowing communication between the intracellular and extracellular environment. Micron-sized liposome compartments commonly known as giant unilamellar vesicles (GUVs) are used to model the cell membrane and encapsulate biological materials and processes in a cell-like confinement. In the field of bottom-up synthetic biology, many have utilized GUVs as substrates to study various biological processes such as protein-lipid interactions, cytoskeletal assembly, and dynamics of protein synthesis. Like cells, it is ideal that GUVs are also mechanically durable and able to stay intact when the inner and outer environment changes. As a result, studies have demonstrated approaches to tune the mechanical properties of GUVs by modulating membrane composition and lumenal material property. In this context, there have been many different methods developed to test the mechanical properties of GUVs. In this review, we will survey various perturbation techniques employed to mechanically characterize GUVs.
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spelling pubmed-98169132023-01-18 Methods to mechanically perturb and characterize GUV-based minimal cell models Wubshet, Nadab H. Liu, Allen P. Comput Struct Biotechnol J Mini Review Cells shield organelles and the cytosol via an active boundary predominantly made of phospholipids and membrane proteins, yet allowing communication between the intracellular and extracellular environment. Micron-sized liposome compartments commonly known as giant unilamellar vesicles (GUVs) are used to model the cell membrane and encapsulate biological materials and processes in a cell-like confinement. In the field of bottom-up synthetic biology, many have utilized GUVs as substrates to study various biological processes such as protein-lipid interactions, cytoskeletal assembly, and dynamics of protein synthesis. Like cells, it is ideal that GUVs are also mechanically durable and able to stay intact when the inner and outer environment changes. As a result, studies have demonstrated approaches to tune the mechanical properties of GUVs by modulating membrane composition and lumenal material property. In this context, there have been many different methods developed to test the mechanical properties of GUVs. In this review, we will survey various perturbation techniques employed to mechanically characterize GUVs. Research Network of Computational and Structural Biotechnology 2022-12-18 /pmc/articles/PMC9816913/ /pubmed/36659916 http://dx.doi.org/10.1016/j.csbj.2022.12.025 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Mini Review
Wubshet, Nadab H.
Liu, Allen P.
Methods to mechanically perturb and characterize GUV-based minimal cell models
title Methods to mechanically perturb and characterize GUV-based minimal cell models
title_full Methods to mechanically perturb and characterize GUV-based minimal cell models
title_fullStr Methods to mechanically perturb and characterize GUV-based minimal cell models
title_full_unstemmed Methods to mechanically perturb and characterize GUV-based minimal cell models
title_short Methods to mechanically perturb and characterize GUV-based minimal cell models
title_sort methods to mechanically perturb and characterize guv-based minimal cell models
topic Mini Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9816913/
https://www.ncbi.nlm.nih.gov/pubmed/36659916
http://dx.doi.org/10.1016/j.csbj.2022.12.025
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