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Novel strategy for primary epithelial cell isolation: Combination of hyaluronidase and collagenase I

OBJECTIVE: Different strategies for epithelial cell isolation significantly affect the viability and physiological properties of primary cells. Trypsin digestion, a conventional method, causes collateral damage owing to its strong digestive potential. To better preserve the physiological properties...

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Detalles Bibliográficos
Autores principales: Hu, Zhewen, Chen, Yiming, Gao, Min, Chi, Xiaopei, He, Ying, Zhang, Chenguang, Yang, Yue, Li, Yuman, Lv, Yan, Huang, Ying, Deng, Xuliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9816927/
https://www.ncbi.nlm.nih.gov/pubmed/35920005
http://dx.doi.org/10.1111/cpr.13320