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Raman microscopy tracks maturity of melanin intermediates in Botrytis cinerea, a plant pathogen

We use Raman microscopy to describe the structure and chemical composition of both conidiophore and hyphae of Botrytis cinerea, a common plant pathogen. To interpret experimental data, we use density functional theory (DFT) to compute Raman tensors specific to an important fungal glycopeptide, a seg...

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Detalles Bibliográficos
Autores principales: Volkov, Victor V., Sadaf, Ayesha, Perry, Carole C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9817083/
https://www.ncbi.nlm.nih.gov/pubmed/36686955
http://dx.doi.org/10.1039/d2ra06439a
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author Volkov, Victor V.
Sadaf, Ayesha
Perry, Carole C.
author_facet Volkov, Victor V.
Sadaf, Ayesha
Perry, Carole C.
author_sort Volkov, Victor V.
collection PubMed
description We use Raman microscopy to describe the structure and chemical composition of both conidiophore and hyphae of Botrytis cinerea, a common plant pathogen. To interpret experimental data, we use density functional theory (DFT) to compute Raman tensors specific to an important fungal glycopeptide, a segment of α-chitin, and several naphthalene-based precursors of increasing complexity, which we propose play a role in the melanin synthesis pathway. Using spectral interpretations based on quantum chemical validation, we review microscopy images reconstructed for specific Raman activities and describe differences in distributions of structural components, photo-protective secondary naphthalene-based pigments, and proteins in both spores and hyphal filaments. Comparison of our results with literature data on other fungi suggests an example of convergent evolution expressed at the level of secondary metabolites specific to plant pathogenic fungi. Our results indicate that pre-resonant Raman monitoring of melanin precursors may help assessment of local Botrytis population biology to aid agricultural production.
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spelling pubmed-98170832023-01-20 Raman microscopy tracks maturity of melanin intermediates in Botrytis cinerea, a plant pathogen Volkov, Victor V. Sadaf, Ayesha Perry, Carole C. RSC Adv Chemistry We use Raman microscopy to describe the structure and chemical composition of both conidiophore and hyphae of Botrytis cinerea, a common plant pathogen. To interpret experimental data, we use density functional theory (DFT) to compute Raman tensors specific to an important fungal glycopeptide, a segment of α-chitin, and several naphthalene-based precursors of increasing complexity, which we propose play a role in the melanin synthesis pathway. Using spectral interpretations based on quantum chemical validation, we review microscopy images reconstructed for specific Raman activities and describe differences in distributions of structural components, photo-protective secondary naphthalene-based pigments, and proteins in both spores and hyphal filaments. Comparison of our results with literature data on other fungi suggests an example of convergent evolution expressed at the level of secondary metabolites specific to plant pathogenic fungi. Our results indicate that pre-resonant Raman monitoring of melanin precursors may help assessment of local Botrytis population biology to aid agricultural production. The Royal Society of Chemistry 2023-01-06 /pmc/articles/PMC9817083/ /pubmed/36686955 http://dx.doi.org/10.1039/d2ra06439a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Volkov, Victor V.
Sadaf, Ayesha
Perry, Carole C.
Raman microscopy tracks maturity of melanin intermediates in Botrytis cinerea, a plant pathogen
title Raman microscopy tracks maturity of melanin intermediates in Botrytis cinerea, a plant pathogen
title_full Raman microscopy tracks maturity of melanin intermediates in Botrytis cinerea, a plant pathogen
title_fullStr Raman microscopy tracks maturity of melanin intermediates in Botrytis cinerea, a plant pathogen
title_full_unstemmed Raman microscopy tracks maturity of melanin intermediates in Botrytis cinerea, a plant pathogen
title_short Raman microscopy tracks maturity of melanin intermediates in Botrytis cinerea, a plant pathogen
title_sort raman microscopy tracks maturity of melanin intermediates in botrytis cinerea, a plant pathogen
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9817083/
https://www.ncbi.nlm.nih.gov/pubmed/36686955
http://dx.doi.org/10.1039/d2ra06439a
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