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Comprehensive analysis of the significance of METTL7A gene in the prognosis of lung adenocarcinoma

BACKGROUND: The most common subtype of lung cancer, called lung adenocarcinoma (LUAD), is also the largest cause of cancer death in the world. The aim of this study was to determine the importance of the METTL7A gene in the prognosis of patients with LUAD. METHODS: This particular study used a total...

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Autores principales: Pan, Ya-Qiang, Xiao, Ying, Li, Zhenhua, Tao, Long, Chen, Ge, Zhu, Jing-Feng, Lv, Lu, Liu, Jian-Chao, Qi, Jun-Qing, Shao, AiZhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9817104/
https://www.ncbi.nlm.nih.gov/pubmed/36620541
http://dx.doi.org/10.3389/fonc.2022.1071100
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author Pan, Ya-Qiang
Xiao, Ying
Li, Zhenhua
Tao, Long
Chen, Ge
Zhu, Jing-Feng
Lv, Lu
Liu, Jian-Chao
Qi, Jun-Qing
Shao, AiZhong
author_facet Pan, Ya-Qiang
Xiao, Ying
Li, Zhenhua
Tao, Long
Chen, Ge
Zhu, Jing-Feng
Lv, Lu
Liu, Jian-Chao
Qi, Jun-Qing
Shao, AiZhong
author_sort Pan, Ya-Qiang
collection PubMed
description BACKGROUND: The most common subtype of lung cancer, called lung adenocarcinoma (LUAD), is also the largest cause of cancer death in the world. The aim of this study was to determine the importance of the METTL7A gene in the prognosis of patients with LUAD. METHODS: This particular study used a total of four different LUAD datasets, namely TCGA-LUAD, GSE32863, GSE31210 and GSE13213. Using RT-qPCR, we were able to determine METTL7A expression levels in clinical samples. Univariate and multivariate Cox regression analyses were used to identify factors with independent effects on prognosis in patients with LUAD, and nomograms were designed to predict survival in these patients. Using gene set variation analysis (GSVA), we investigated differences in enriched pathways between METTL7A high and low expression groups. Microenvironmental cell population counter (MCP-counter) and single-sample gene set enrichment analysis (ssGSEA) methods were used to study immune infiltration in LUAD samples. Using the ESTIMATE technique, we were able to determine the immune score, stromal score, and estimated score for each LUAD patient. A competing endogenous RNA network, also known as ceRNA, was established with the help of the Cytoscape program. RESULTS: We detected that METTL7A was down-regulated in pan-cancer, including LUAD. The survival study indicates that METTL7A was a protective factor in the prognosis of LUAD. The univariate and multivariate Cox regression analyses revealed that METTL7A was a robust independent prognostic indicator in survival prediction. Through the use of GSVA, several immune-related pathways were shown to be enriched in both the high-expression and low-expression groups of METTL7A. Analysis of the tumor microenvironment revealed that the immune microenvironment of the group with low expression was suppressed, which may be connected to the poor prognosis. To explore the ceRNA regulatory mechanism of METTL7A, we finally constructed a regulatory network containing 1 mRNA, 2 miRNAs, and 5 long non-coding RNAs (lncRNAs). CONCLUSION: In conclusion, we presented METTL7A as a potential and promising prognostic indicator of LUAD. This biomarker has the potential to offer us with a comprehensive perspective of the prediction of prognosis and treatment for LUAD patients.
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spelling pubmed-98171042023-01-07 Comprehensive analysis of the significance of METTL7A gene in the prognosis of lung adenocarcinoma Pan, Ya-Qiang Xiao, Ying Li, Zhenhua Tao, Long Chen, Ge Zhu, Jing-Feng Lv, Lu Liu, Jian-Chao Qi, Jun-Qing Shao, AiZhong Front Oncol Oncology BACKGROUND: The most common subtype of lung cancer, called lung adenocarcinoma (LUAD), is also the largest cause of cancer death in the world. The aim of this study was to determine the importance of the METTL7A gene in the prognosis of patients with LUAD. METHODS: This particular study used a total of four different LUAD datasets, namely TCGA-LUAD, GSE32863, GSE31210 and GSE13213. Using RT-qPCR, we were able to determine METTL7A expression levels in clinical samples. Univariate and multivariate Cox regression analyses were used to identify factors with independent effects on prognosis in patients with LUAD, and nomograms were designed to predict survival in these patients. Using gene set variation analysis (GSVA), we investigated differences in enriched pathways between METTL7A high and low expression groups. Microenvironmental cell population counter (MCP-counter) and single-sample gene set enrichment analysis (ssGSEA) methods were used to study immune infiltration in LUAD samples. Using the ESTIMATE technique, we were able to determine the immune score, stromal score, and estimated score for each LUAD patient. A competing endogenous RNA network, also known as ceRNA, was established with the help of the Cytoscape program. RESULTS: We detected that METTL7A was down-regulated in pan-cancer, including LUAD. The survival study indicates that METTL7A was a protective factor in the prognosis of LUAD. The univariate and multivariate Cox regression analyses revealed that METTL7A was a robust independent prognostic indicator in survival prediction. Through the use of GSVA, several immune-related pathways were shown to be enriched in both the high-expression and low-expression groups of METTL7A. Analysis of the tumor microenvironment revealed that the immune microenvironment of the group with low expression was suppressed, which may be connected to the poor prognosis. To explore the ceRNA regulatory mechanism of METTL7A, we finally constructed a regulatory network containing 1 mRNA, 2 miRNAs, and 5 long non-coding RNAs (lncRNAs). CONCLUSION: In conclusion, we presented METTL7A as a potential and promising prognostic indicator of LUAD. This biomarker has the potential to offer us with a comprehensive perspective of the prediction of prognosis and treatment for LUAD patients. Frontiers Media S.A. 2022-12-23 /pmc/articles/PMC9817104/ /pubmed/36620541 http://dx.doi.org/10.3389/fonc.2022.1071100 Text en Copyright © 2022 Pan, Xiao, Li, Tao, Chen, Zhu, Lv, Liu, Qi and Shao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Pan, Ya-Qiang
Xiao, Ying
Li, Zhenhua
Tao, Long
Chen, Ge
Zhu, Jing-Feng
Lv, Lu
Liu, Jian-Chao
Qi, Jun-Qing
Shao, AiZhong
Comprehensive analysis of the significance of METTL7A gene in the prognosis of lung adenocarcinoma
title Comprehensive analysis of the significance of METTL7A gene in the prognosis of lung adenocarcinoma
title_full Comprehensive analysis of the significance of METTL7A gene in the prognosis of lung adenocarcinoma
title_fullStr Comprehensive analysis of the significance of METTL7A gene in the prognosis of lung adenocarcinoma
title_full_unstemmed Comprehensive analysis of the significance of METTL7A gene in the prognosis of lung adenocarcinoma
title_short Comprehensive analysis of the significance of METTL7A gene in the prognosis of lung adenocarcinoma
title_sort comprehensive analysis of the significance of mettl7a gene in the prognosis of lung adenocarcinoma
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9817104/
https://www.ncbi.nlm.nih.gov/pubmed/36620541
http://dx.doi.org/10.3389/fonc.2022.1071100
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