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ASV portal: an interface to DNA-based biodiversity data in the Living Atlas

BACKGROUND: The Living Atlas is an open source platform used to collect, visualise and analyse biodiversity data from multiple sources, and serves as the national biodiversity data hub in many countries. Although powerful, the Living Atlas has had limited functionality for species occurrence data de...

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Autores principales: Prager, Maria, Lundin, Daniel, Ronquist, Fredrik, Andersson, Anders F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9817246/
https://www.ncbi.nlm.nih.gov/pubmed/36604610
http://dx.doi.org/10.1186/s12859-022-05120-z
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author Prager, Maria
Lundin, Daniel
Ronquist, Fredrik
Andersson, Anders F.
author_facet Prager, Maria
Lundin, Daniel
Ronquist, Fredrik
Andersson, Anders F.
author_sort Prager, Maria
collection PubMed
description BACKGROUND: The Living Atlas is an open source platform used to collect, visualise and analyse biodiversity data from multiple sources, and serves as the national biodiversity data hub in many countries. Although powerful, the Living Atlas has had limited functionality for species occurrence data derived from DNA sequences. As a step toward integrating this fast-growing data source into the platform, we developed the Amplicon Sequence Variant (ASV) portal: a web interface to sequence-based biodiversity observations in the Living Atlas. RESULTS: The ASV portal allows data providers to submit denoised metabarcoding output to the Living Atlas platform via an intermediary ASV database. It also enables users to search for existing ASVs and associated Living Atlas records using the Basic Local Alignment Search Tool, or via filters on taxonomy and sequencing details. The ASV portal is a Python-Flask/jQuery web interface, implemented as a multi-container docker service, and is an integral part of the Swedish Biodiversity Data Infrastructure. CONCLUSION: The ASV portal is a web interface that effectively integrates biodiversity data derived from DNA sequences into the Living Atlas platform.
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spelling pubmed-98172462023-01-07 ASV portal: an interface to DNA-based biodiversity data in the Living Atlas Prager, Maria Lundin, Daniel Ronquist, Fredrik Andersson, Anders F. BMC Bioinformatics Software BACKGROUND: The Living Atlas is an open source platform used to collect, visualise and analyse biodiversity data from multiple sources, and serves as the national biodiversity data hub in many countries. Although powerful, the Living Atlas has had limited functionality for species occurrence data derived from DNA sequences. As a step toward integrating this fast-growing data source into the platform, we developed the Amplicon Sequence Variant (ASV) portal: a web interface to sequence-based biodiversity observations in the Living Atlas. RESULTS: The ASV portal allows data providers to submit denoised metabarcoding output to the Living Atlas platform via an intermediary ASV database. It also enables users to search for existing ASVs and associated Living Atlas records using the Basic Local Alignment Search Tool, or via filters on taxonomy and sequencing details. The ASV portal is a Python-Flask/jQuery web interface, implemented as a multi-container docker service, and is an integral part of the Swedish Biodiversity Data Infrastructure. CONCLUSION: The ASV portal is a web interface that effectively integrates biodiversity data derived from DNA sequences into the Living Atlas platform. BioMed Central 2023-01-05 /pmc/articles/PMC9817246/ /pubmed/36604610 http://dx.doi.org/10.1186/s12859-022-05120-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Software
Prager, Maria
Lundin, Daniel
Ronquist, Fredrik
Andersson, Anders F.
ASV portal: an interface to DNA-based biodiversity data in the Living Atlas
title ASV portal: an interface to DNA-based biodiversity data in the Living Atlas
title_full ASV portal: an interface to DNA-based biodiversity data in the Living Atlas
title_fullStr ASV portal: an interface to DNA-based biodiversity data in the Living Atlas
title_full_unstemmed ASV portal: an interface to DNA-based biodiversity data in the Living Atlas
title_short ASV portal: an interface to DNA-based biodiversity data in the Living Atlas
title_sort asv portal: an interface to dna-based biodiversity data in the living atlas
topic Software
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9817246/
https://www.ncbi.nlm.nih.gov/pubmed/36604610
http://dx.doi.org/10.1186/s12859-022-05120-z
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