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Laminin-binding protein of Streptococcus suis serotype 2 influences zinc acquisition and cytokine responses

Streptococcus suis serotype 2 is an important bacterial pathogen of swine, responsible for substantial economic losses to the swine industry worldwide. The knowledge on the pathogenesis of the infection caused by S. suis is still poorly known. It has been previously described that S. suis possesses...

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Autores principales: Payen, Servane, Rrodriguez, Jesús Aranda, Segura, Mariela, Gottschalk, Marcelo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9817373/
https://www.ncbi.nlm.nih.gov/pubmed/36604750
http://dx.doi.org/10.1186/s13567-022-01128-8
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author Payen, Servane
Rrodriguez, Jesús Aranda
Segura, Mariela
Gottschalk, Marcelo
author_facet Payen, Servane
Rrodriguez, Jesús Aranda
Segura, Mariela
Gottschalk, Marcelo
author_sort Payen, Servane
collection PubMed
description Streptococcus suis serotype 2 is an important bacterial pathogen of swine, responsible for substantial economic losses to the swine industry worldwide. The knowledge on the pathogenesis of the infection caused by S. suis is still poorly known. It has been previously described that S. suis possesses at least one lipoprotein with double laminin and zinc (Zn)-binding properties, which was described in the literature as either laminin-binding protein (Lmb, as in the current study), lipoprotein 103, CDS 0330 or AdcAII. In the present study, the role of the Lmb in the pathogenesis of the infection caused by S. suis serotype 2 was dissected. Using isogenic mutants, results showed that Lmb does not play an important role in the laminin-binding activity of S. suis, even when clearly exposed at the bacterial surface. In addition, the presence of this lipoprotein does not influence bacterial adhesion to and invasion of porcine respiratory epithelial and brain endothelial cells and it does not increase the susceptibility of S. suis to phagocytosis. On the other hand, the Lmb was shown to play an important role as cytokine activator when tested in vitro with dendritic cells. Finally, this lipoprotein plays a critical role in Zn acquisition from the host environment allowing bacteria to grow in vivo. The significant lower virulence of the Lmb defective mutant may be related to a combination of a lower bacterial survival due to the incapacity to acquire Zn from their surrounding milieu and a reduced cytokine activation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13567-022-01128-8.
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spelling pubmed-98173732023-01-07 Laminin-binding protein of Streptococcus suis serotype 2 influences zinc acquisition and cytokine responses Payen, Servane Rrodriguez, Jesús Aranda Segura, Mariela Gottschalk, Marcelo Vet Res Research Article Streptococcus suis serotype 2 is an important bacterial pathogen of swine, responsible for substantial economic losses to the swine industry worldwide. The knowledge on the pathogenesis of the infection caused by S. suis is still poorly known. It has been previously described that S. suis possesses at least one lipoprotein with double laminin and zinc (Zn)-binding properties, which was described in the literature as either laminin-binding protein (Lmb, as in the current study), lipoprotein 103, CDS 0330 or AdcAII. In the present study, the role of the Lmb in the pathogenesis of the infection caused by S. suis serotype 2 was dissected. Using isogenic mutants, results showed that Lmb does not play an important role in the laminin-binding activity of S. suis, even when clearly exposed at the bacterial surface. In addition, the presence of this lipoprotein does not influence bacterial adhesion to and invasion of porcine respiratory epithelial and brain endothelial cells and it does not increase the susceptibility of S. suis to phagocytosis. On the other hand, the Lmb was shown to play an important role as cytokine activator when tested in vitro with dendritic cells. Finally, this lipoprotein plays a critical role in Zn acquisition from the host environment allowing bacteria to grow in vivo. The significant lower virulence of the Lmb defective mutant may be related to a combination of a lower bacterial survival due to the incapacity to acquire Zn from their surrounding milieu and a reduced cytokine activation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13567-022-01128-8. BioMed Central 2023-01-05 2023 /pmc/articles/PMC9817373/ /pubmed/36604750 http://dx.doi.org/10.1186/s13567-022-01128-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Payen, Servane
Rrodriguez, Jesús Aranda
Segura, Mariela
Gottschalk, Marcelo
Laminin-binding protein of Streptococcus suis serotype 2 influences zinc acquisition and cytokine responses
title Laminin-binding protein of Streptococcus suis serotype 2 influences zinc acquisition and cytokine responses
title_full Laminin-binding protein of Streptococcus suis serotype 2 influences zinc acquisition and cytokine responses
title_fullStr Laminin-binding protein of Streptococcus suis serotype 2 influences zinc acquisition and cytokine responses
title_full_unstemmed Laminin-binding protein of Streptococcus suis serotype 2 influences zinc acquisition and cytokine responses
title_short Laminin-binding protein of Streptococcus suis serotype 2 influences zinc acquisition and cytokine responses
title_sort laminin-binding protein of streptococcus suis serotype 2 influences zinc acquisition and cytokine responses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9817373/
https://www.ncbi.nlm.nih.gov/pubmed/36604750
http://dx.doi.org/10.1186/s13567-022-01128-8
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