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Gut microbiome and stages of diabetes in middle-aged adults: CARDIA microbiome study
BACKGROUND: Animal and human studies suggest the gut microbiome is linked to diabetes but additional data are needed on the associations of the gut microbiome to specific diabetes characteristics. The aim of this study was to examine the associations of gut microbiome composition to insulin resistan...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9817375/ https://www.ncbi.nlm.nih.gov/pubmed/36604708 http://dx.doi.org/10.1186/s12986-022-00721-0 |
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author | Hu, Yi-Han Meyer, Katie Lulla, Anju Lewis, Cora E. Carnethon, Mercedes R. Schreiner, Pamela J. Sidney, Stephen Shikany, James M. Meirelles, Osorio Launer, Lenore J. |
author_facet | Hu, Yi-Han Meyer, Katie Lulla, Anju Lewis, Cora E. Carnethon, Mercedes R. Schreiner, Pamela J. Sidney, Stephen Shikany, James M. Meirelles, Osorio Launer, Lenore J. |
author_sort | Hu, Yi-Han |
collection | PubMed |
description | BACKGROUND: Animal and human studies suggest the gut microbiome is linked to diabetes but additional data are needed on the associations of the gut microbiome to specific diabetes characteristics. The aim of this study was to examine the associations of gut microbiome composition to insulin resistance [Homeostatic Model Assessment of Insulin Resistance (HOMA-IR)], duration of diabetes, and 4 stages of diabetes [normoglycemia, pre-diabetes, and diabetes with (+) and without (−) medication for diabetes]. METHODS: Data are from a sub-sample (n = 605) of Black and White participants from the 30-year follow-up exam of the prospectively followed community-based Coronary Artery Risk Development in Young Adults cohort (2015–2016; aged 48–60 years). Stool samples were collected and sequenced using the 16S ribosomal RNA method. Microbial measures included: α diversity (within-person), β diversity (between-person), and taxonomies. All analyses were adjusted for demographic, clinical, lifestyle factors, and use of relevant medications (full adjustment). Multivariate linear regression models were used to assess the association of diabetes characteristics with α diversity and genera abundance, while the association with β diversity was analyzed using permutational multivariate analysis of variance. Statistical significance was set to p-value < 0.05 for α and β diversity analyses and to q-value < 0.1 for genera abundance analyses. RESULTS: There were 16.7% of participants with pre-diabetes, and 14.4% with diabetes (9% diabetes+) with median (interquartile range) diabetes duration of 5 (5–10) years. In the fully adjusted models, compared to those with no diabetes, longer diabetes duration and the diabetes + group had a lower α diversity. There were significant differences in β diversity across diabetes-related characteristics. A significantly reduced abundance of butyrate-producing genera was associated with higher HOMA-IR (ex., Anaerostipes and Lachnospiraceae_UCG.004), longer diabetes duration (ex., Agathobacter and Ruminococcus), and diabetes + (ex., Faecalibacterium and Romboutsia). CONCLUSIONS: Our results suggest that an adverse alteration of gut microbiome composition is related to higher insulin resistance, longer diabetes duration, and is present in those persons with diabetes using medications. These diabetes-related characteristics were also associated with lower levels of certain butyrate-producing bacteria that produce health-promoting short‐chain fatty acids. Understanding the role of gut microbiota in glucose regulation may provide new strategies to reduce the burden of diabetes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12986-022-00721-0. |
format | Online Article Text |
id | pubmed-9817375 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-98173752023-01-07 Gut microbiome and stages of diabetes in middle-aged adults: CARDIA microbiome study Hu, Yi-Han Meyer, Katie Lulla, Anju Lewis, Cora E. Carnethon, Mercedes R. Schreiner, Pamela J. Sidney, Stephen Shikany, James M. Meirelles, Osorio Launer, Lenore J. Nutr Metab (Lond) Research BACKGROUND: Animal and human studies suggest the gut microbiome is linked to diabetes but additional data are needed on the associations of the gut microbiome to specific diabetes characteristics. The aim of this study was to examine the associations of gut microbiome composition to insulin resistance [Homeostatic Model Assessment of Insulin Resistance (HOMA-IR)], duration of diabetes, and 4 stages of diabetes [normoglycemia, pre-diabetes, and diabetes with (+) and without (−) medication for diabetes]. METHODS: Data are from a sub-sample (n = 605) of Black and White participants from the 30-year follow-up exam of the prospectively followed community-based Coronary Artery Risk Development in Young Adults cohort (2015–2016; aged 48–60 years). Stool samples were collected and sequenced using the 16S ribosomal RNA method. Microbial measures included: α diversity (within-person), β diversity (between-person), and taxonomies. All analyses were adjusted for demographic, clinical, lifestyle factors, and use of relevant medications (full adjustment). Multivariate linear regression models were used to assess the association of diabetes characteristics with α diversity and genera abundance, while the association with β diversity was analyzed using permutational multivariate analysis of variance. Statistical significance was set to p-value < 0.05 for α and β diversity analyses and to q-value < 0.1 for genera abundance analyses. RESULTS: There were 16.7% of participants with pre-diabetes, and 14.4% with diabetes (9% diabetes+) with median (interquartile range) diabetes duration of 5 (5–10) years. In the fully adjusted models, compared to those with no diabetes, longer diabetes duration and the diabetes + group had a lower α diversity. There were significant differences in β diversity across diabetes-related characteristics. A significantly reduced abundance of butyrate-producing genera was associated with higher HOMA-IR (ex., Anaerostipes and Lachnospiraceae_UCG.004), longer diabetes duration (ex., Agathobacter and Ruminococcus), and diabetes + (ex., Faecalibacterium and Romboutsia). CONCLUSIONS: Our results suggest that an adverse alteration of gut microbiome composition is related to higher insulin resistance, longer diabetes duration, and is present in those persons with diabetes using medications. These diabetes-related characteristics were also associated with lower levels of certain butyrate-producing bacteria that produce health-promoting short‐chain fatty acids. Understanding the role of gut microbiota in glucose regulation may provide new strategies to reduce the burden of diabetes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12986-022-00721-0. BioMed Central 2023-01-05 /pmc/articles/PMC9817375/ /pubmed/36604708 http://dx.doi.org/10.1186/s12986-022-00721-0 Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Hu, Yi-Han Meyer, Katie Lulla, Anju Lewis, Cora E. Carnethon, Mercedes R. Schreiner, Pamela J. Sidney, Stephen Shikany, James M. Meirelles, Osorio Launer, Lenore J. Gut microbiome and stages of diabetes in middle-aged adults: CARDIA microbiome study |
title | Gut microbiome and stages of diabetes in middle-aged adults: CARDIA microbiome study |
title_full | Gut microbiome and stages of diabetes in middle-aged adults: CARDIA microbiome study |
title_fullStr | Gut microbiome and stages of diabetes in middle-aged adults: CARDIA microbiome study |
title_full_unstemmed | Gut microbiome and stages of diabetes in middle-aged adults: CARDIA microbiome study |
title_short | Gut microbiome and stages of diabetes in middle-aged adults: CARDIA microbiome study |
title_sort | gut microbiome and stages of diabetes in middle-aged adults: cardia microbiome study |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9817375/ https://www.ncbi.nlm.nih.gov/pubmed/36604708 http://dx.doi.org/10.1186/s12986-022-00721-0 |
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