Cargando…

Evaluating Ruminal and Small Intestinal Morphology and Microbiota Composition of Calves Fed a Macleaya cordata Extract Preparation

SIMPLE SUMMARY: Mucosal morphology and microbiota composition are key components of the gut development of calves. Adverse health events during pre-weaning and diet changes at weaning can create a lasting impact on those components. Phytogenic feed additives such as the Macleaya cordata plant extrac...

Descripción completa

Detalles Bibliográficos
Autores principales: Wickramasinghe, Janaka, Anderson, Chiron J., Kaya, Can Ayhan, Gorden, Patrick J., Ribeiro, Flavio Rodrigues Borges, Dohms, Juliane, Rigert, Sydney, Schmitz-Esser, Stephan, Appuhamy, Ranga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9817497/
https://www.ncbi.nlm.nih.gov/pubmed/36611664
http://dx.doi.org/10.3390/ani13010054
_version_ 1784864764486221824
author Wickramasinghe, Janaka
Anderson, Chiron J.
Kaya, Can Ayhan
Gorden, Patrick J.
Ribeiro, Flavio Rodrigues Borges
Dohms, Juliane
Rigert, Sydney
Schmitz-Esser, Stephan
Appuhamy, Ranga
author_facet Wickramasinghe, Janaka
Anderson, Chiron J.
Kaya, Can Ayhan
Gorden, Patrick J.
Ribeiro, Flavio Rodrigues Borges
Dohms, Juliane
Rigert, Sydney
Schmitz-Esser, Stephan
Appuhamy, Ranga
author_sort Wickramasinghe, Janaka
collection PubMed
description SIMPLE SUMMARY: Mucosal morphology and microbiota composition are key components of the gut development of calves. Adverse health events during pre-weaning and diet changes at weaning can create a lasting impact on those components. Phytogenic feed additives such as the Macleaya cordata plant extract preparations (MCE) improved the gut morphology and microbiota composition of pigs and poultry in previous studies. In this study, MCE was fed to dairy × beef crossbred calves before (6 to 45 d) and after weaning (45 to 90 d). The cumulative impact on the ruminal and small intestinal morphology and microbiota composition were evaluated at 95 d of age. Despite the minor changes in ruminal and intestinal microbial populations, MCE increased the rumen papillae length and villus height: crypt depth in the small intestine, suggesting the potential to enhance gut health and development. ABSTRACT: The objective was to determine the impact of feeding MCE on ruminal and intestinal morphology and microbiota composition of calves. A total of 10 male and 10 female crossbred (dairy × beef) calves (6 d of age) were assigned randomly to control (CTL; n = 10) or MCE-supplemented (TRT; n = 10) groups. The MCE was fed in the milk replacer and top-dressed on the calf starter during pre-weaning (6 to 49 d) and post-weaning (50 to 95 d) periods, respectively. Calves were slaughtered at 95 d to collect rumen and intestinal samples to determine volatile fatty acid (VFA) profile, mucosal morphology, and microbiota composition. The effects of MCE were analyzed by accounting for the sex and breed effects. Feeding MCE increased rumen papillae length (p = 0.010) and intestinal villus height: crypt depth (p < 0.030) compared to CTL but did not affect rumen VFA profile. The TRT had a negligible impact on microbial community composition in both the rumen and the jejunum. In conclusion, feeding MCE from birth through weaning can improve ruminal and small intestinal mucosa development of calves despite the negligible microbiota composition changes observed post-weaning.
format Online
Article
Text
id pubmed-9817497
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-98174972023-01-07 Evaluating Ruminal and Small Intestinal Morphology and Microbiota Composition of Calves Fed a Macleaya cordata Extract Preparation Wickramasinghe, Janaka Anderson, Chiron J. Kaya, Can Ayhan Gorden, Patrick J. Ribeiro, Flavio Rodrigues Borges Dohms, Juliane Rigert, Sydney Schmitz-Esser, Stephan Appuhamy, Ranga Animals (Basel) Article SIMPLE SUMMARY: Mucosal morphology and microbiota composition are key components of the gut development of calves. Adverse health events during pre-weaning and diet changes at weaning can create a lasting impact on those components. Phytogenic feed additives such as the Macleaya cordata plant extract preparations (MCE) improved the gut morphology and microbiota composition of pigs and poultry in previous studies. In this study, MCE was fed to dairy × beef crossbred calves before (6 to 45 d) and after weaning (45 to 90 d). The cumulative impact on the ruminal and small intestinal morphology and microbiota composition were evaluated at 95 d of age. Despite the minor changes in ruminal and intestinal microbial populations, MCE increased the rumen papillae length and villus height: crypt depth in the small intestine, suggesting the potential to enhance gut health and development. ABSTRACT: The objective was to determine the impact of feeding MCE on ruminal and intestinal morphology and microbiota composition of calves. A total of 10 male and 10 female crossbred (dairy × beef) calves (6 d of age) were assigned randomly to control (CTL; n = 10) or MCE-supplemented (TRT; n = 10) groups. The MCE was fed in the milk replacer and top-dressed on the calf starter during pre-weaning (6 to 49 d) and post-weaning (50 to 95 d) periods, respectively. Calves were slaughtered at 95 d to collect rumen and intestinal samples to determine volatile fatty acid (VFA) profile, mucosal morphology, and microbiota composition. The effects of MCE were analyzed by accounting for the sex and breed effects. Feeding MCE increased rumen papillae length (p = 0.010) and intestinal villus height: crypt depth (p < 0.030) compared to CTL but did not affect rumen VFA profile. The TRT had a negligible impact on microbial community composition in both the rumen and the jejunum. In conclusion, feeding MCE from birth through weaning can improve ruminal and small intestinal mucosa development of calves despite the negligible microbiota composition changes observed post-weaning. MDPI 2022-12-23 /pmc/articles/PMC9817497/ /pubmed/36611664 http://dx.doi.org/10.3390/ani13010054 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wickramasinghe, Janaka
Anderson, Chiron J.
Kaya, Can Ayhan
Gorden, Patrick J.
Ribeiro, Flavio Rodrigues Borges
Dohms, Juliane
Rigert, Sydney
Schmitz-Esser, Stephan
Appuhamy, Ranga
Evaluating Ruminal and Small Intestinal Morphology and Microbiota Composition of Calves Fed a Macleaya cordata Extract Preparation
title Evaluating Ruminal and Small Intestinal Morphology and Microbiota Composition of Calves Fed a Macleaya cordata Extract Preparation
title_full Evaluating Ruminal and Small Intestinal Morphology and Microbiota Composition of Calves Fed a Macleaya cordata Extract Preparation
title_fullStr Evaluating Ruminal and Small Intestinal Morphology and Microbiota Composition of Calves Fed a Macleaya cordata Extract Preparation
title_full_unstemmed Evaluating Ruminal and Small Intestinal Morphology and Microbiota Composition of Calves Fed a Macleaya cordata Extract Preparation
title_short Evaluating Ruminal and Small Intestinal Morphology and Microbiota Composition of Calves Fed a Macleaya cordata Extract Preparation
title_sort evaluating ruminal and small intestinal morphology and microbiota composition of calves fed a macleaya cordata extract preparation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9817497/
https://www.ncbi.nlm.nih.gov/pubmed/36611664
http://dx.doi.org/10.3390/ani13010054
work_keys_str_mv AT wickramasinghejanaka evaluatingruminalandsmallintestinalmorphologyandmicrobiotacompositionofcalvesfedamacleayacordataextractpreparation
AT andersonchironj evaluatingruminalandsmallintestinalmorphologyandmicrobiotacompositionofcalvesfedamacleayacordataextractpreparation
AT kayacanayhan evaluatingruminalandsmallintestinalmorphologyandmicrobiotacompositionofcalvesfedamacleayacordataextractpreparation
AT gordenpatrickj evaluatingruminalandsmallintestinalmorphologyandmicrobiotacompositionofcalvesfedamacleayacordataextractpreparation
AT ribeiroflaviorodriguesborges evaluatingruminalandsmallintestinalmorphologyandmicrobiotacompositionofcalvesfedamacleayacordataextractpreparation
AT dohmsjuliane evaluatingruminalandsmallintestinalmorphologyandmicrobiotacompositionofcalvesfedamacleayacordataextractpreparation
AT rigertsydney evaluatingruminalandsmallintestinalmorphologyandmicrobiotacompositionofcalvesfedamacleayacordataextractpreparation
AT schmitzesserstephan evaluatingruminalandsmallintestinalmorphologyandmicrobiotacompositionofcalvesfedamacleayacordataextractpreparation
AT appuhamyranga evaluatingruminalandsmallintestinalmorphologyandmicrobiotacompositionofcalvesfedamacleayacordataextractpreparation