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An Epidemiological Investigation and Drug-Resistant Strain Isolation of Nematodirus oiratianus in Sheep in Inner Mongolia, China

SIMPLE SUMMARY: Nematodirus has a serious impact on the gastrointestinal health of ruminants and the economic development of animal husbandry. This study investigated Nematodirus infections of different rearing patterns in sheep in two well-known natural pastures in Inner Mongolia, China. The result...

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Detalles Bibliográficos
Autores principales: Liu, Yang, Wang, Penglong, Wang, Rui, Li, Junyan, Zhai, Bintao, Luo, Xiaoping, Yang, Xiaoye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9817721/
https://www.ncbi.nlm.nih.gov/pubmed/36611640
http://dx.doi.org/10.3390/ani13010030
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author Liu, Yang
Wang, Penglong
Wang, Rui
Li, Junyan
Zhai, Bintao
Luo, Xiaoping
Yang, Xiaoye
author_facet Liu, Yang
Wang, Penglong
Wang, Rui
Li, Junyan
Zhai, Bintao
Luo, Xiaoping
Yang, Xiaoye
author_sort Liu, Yang
collection PubMed
description SIMPLE SUMMARY: Nematodirus has a serious impact on the gastrointestinal health of ruminants and the economic development of animal husbandry. This study investigated Nematodirus infections of different rearing patterns in sheep in two well-known natural pastures in Inner Mongolia, China. The results indicated that the average infection rate was more than 50%. Then, a naturally occurring strain of Nematodirus was obtained by using numerous isolation methods. We recorded the detailed development process of the nematode eggs and tested for drug resistance, and the results showed that the isolated strains were found to be ivermectin-resistant strains. Our study provides useful data for the morphological study of Nematodirus and the prevention and control of nematodes, offering valuable species resources for studying anthelmintic resistance in nematodes. ABSTRACT: Nematodirus causes major economic losses in the development of the livestock industry, as they are common gastrointestinal parasites of cattle, sheep, and other ruminants. The present study investigated Nematodirus infections in sheep in the Hulunbuir and Xilingol Grasslands, two well-known natural pastures in Inner Mongolia, China. The results indicated that the average infection rate was more than 50%. Furthermore, a naturally occurring strain of Nematodirus was obtained using numerous isolation methods in the survey region. Conventional morphology and molecular biology were used to identify the strain. The larval migration inhibition test was used to determine the ivermectin level in the isolated strain. The results indicated that the larvae belonged to the species Nematodirus oiratianus. The strain was found to be ivermectin-resistant. Thus, these results recorded the detailed development processes of Nematodirus eggs, providing novel insights regarding the prevention and control of nematodes by using conventional anthelmintic regimens and by offering valuable species resources for studying anthelmintic resistance in nematodes.
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spelling pubmed-98177212023-01-07 An Epidemiological Investigation and Drug-Resistant Strain Isolation of Nematodirus oiratianus in Sheep in Inner Mongolia, China Liu, Yang Wang, Penglong Wang, Rui Li, Junyan Zhai, Bintao Luo, Xiaoping Yang, Xiaoye Animals (Basel) Article SIMPLE SUMMARY: Nematodirus has a serious impact on the gastrointestinal health of ruminants and the economic development of animal husbandry. This study investigated Nematodirus infections of different rearing patterns in sheep in two well-known natural pastures in Inner Mongolia, China. The results indicated that the average infection rate was more than 50%. Then, a naturally occurring strain of Nematodirus was obtained by using numerous isolation methods. We recorded the detailed development process of the nematode eggs and tested for drug resistance, and the results showed that the isolated strains were found to be ivermectin-resistant strains. Our study provides useful data for the morphological study of Nematodirus and the prevention and control of nematodes, offering valuable species resources for studying anthelmintic resistance in nematodes. ABSTRACT: Nematodirus causes major economic losses in the development of the livestock industry, as they are common gastrointestinal parasites of cattle, sheep, and other ruminants. The present study investigated Nematodirus infections in sheep in the Hulunbuir and Xilingol Grasslands, two well-known natural pastures in Inner Mongolia, China. The results indicated that the average infection rate was more than 50%. Furthermore, a naturally occurring strain of Nematodirus was obtained using numerous isolation methods in the survey region. Conventional morphology and molecular biology were used to identify the strain. The larval migration inhibition test was used to determine the ivermectin level in the isolated strain. The results indicated that the larvae belonged to the species Nematodirus oiratianus. The strain was found to be ivermectin-resistant. Thus, these results recorded the detailed development processes of Nematodirus eggs, providing novel insights regarding the prevention and control of nematodes by using conventional anthelmintic regimens and by offering valuable species resources for studying anthelmintic resistance in nematodes. MDPI 2022-12-21 /pmc/articles/PMC9817721/ /pubmed/36611640 http://dx.doi.org/10.3390/ani13010030 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Yang
Wang, Penglong
Wang, Rui
Li, Junyan
Zhai, Bintao
Luo, Xiaoping
Yang, Xiaoye
An Epidemiological Investigation and Drug-Resistant Strain Isolation of Nematodirus oiratianus in Sheep in Inner Mongolia, China
title An Epidemiological Investigation and Drug-Resistant Strain Isolation of Nematodirus oiratianus in Sheep in Inner Mongolia, China
title_full An Epidemiological Investigation and Drug-Resistant Strain Isolation of Nematodirus oiratianus in Sheep in Inner Mongolia, China
title_fullStr An Epidemiological Investigation and Drug-Resistant Strain Isolation of Nematodirus oiratianus in Sheep in Inner Mongolia, China
title_full_unstemmed An Epidemiological Investigation and Drug-Resistant Strain Isolation of Nematodirus oiratianus in Sheep in Inner Mongolia, China
title_short An Epidemiological Investigation and Drug-Resistant Strain Isolation of Nematodirus oiratianus in Sheep in Inner Mongolia, China
title_sort epidemiological investigation and drug-resistant strain isolation of nematodirus oiratianus in sheep in inner mongolia, china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9817721/
https://www.ncbi.nlm.nih.gov/pubmed/36611640
http://dx.doi.org/10.3390/ani13010030
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