Size-Mediated Sea Turtle Behavioral Responses at Artificial Habitats in the Northern Gulf of Mexico

SIMPLE SUMMARY: In-water behavioral observation studies of sea turtles are lacking primarily because they are highly migratory throughout their lifecycles. However, utilizing new technology, such as remote stereo-video cameras, allows researchers to collect size-specific data and view relatively natural sea turtle behavior. In this study, we completed stereo-video camera surveys at local artificial reefs, piers, and jetties within the northern Gulf of Mexico from May 2019 to August 2021. We remotely measured sea turtle straight carapace length, assessed wariness using minimum approach distance (MAD) between the turtle and camera operator, and documented behaviors during encounters. Our results indicated that green sea turtles had the smallest mean MAD before a startle response occurred, but the largest range compared to loggerhead and Kemp’s ridley sea turtles. Using a linear mixed model, we found that the size of the turtle was an important predictor of MAD. Overall, our results suggest that larger sea turtles became startled at longer distances compared to the smaller individuals surveyed across all the species studied. This study supports the use of stereo-video camera systems as a non-invasive tool to conduct surveys to assess sea turtle behavioral observations in relation to body size. ABSTRACT: Our understanding of size-specific sea turtle behavior has lagged due to methodological limitations. However, stereo-video cameras (SVC) are an in-water approach that can link body-size and allow for relatively undisturbed behavioral observations. In this study, we conducted SVC dive surveys at local artificial reefs, piers, and jetties in the northern Gulf of Mexico (nGOM) from May 2019 to August 2021. Using SVCs, we measured sea turtle straight carapace length, documented behaviors, and quantified wariness by assessing minimum approach distance (MAD). In green sea turtles (Chelonia mydas), the observed MAD ranged from 0.72 to 5.99 m (mean 2.10 m ± 1.10 standard deviation (SD), n = 73). For loggerhead sea turtles (Caretta caretta), the MAD ranged between 0.93 and 3.80 m (mean 2.12 m ± 0.99 SD, n = 16). Kemp’s ridley sea turtles (Lepidochelys kempii) were similar to loggerheads, and MAD ranged from 0.78 to 3.63 m (mean 2.35 m ± 0.99 SD, n = 8). We then evaluated what biological factors could impact the MAD observed by species, but we excluded Kemp’s ridleys as the sample size was small. Using a linear mixed model and model selection based on AICc, the top ranked model for both green and loggerhead sea turtles included SCL as the most important factor influencing MAD. MAD did not vary with habitat type for either species. Our results showed that larger individuals, regardless of species, have a greater wariness response, becoming startled at greater distances than smaller individuals. The findings of our study support the use of SVC as an accessible, non-invasive tool to conduct ecologically relevant in-water surveys of sea turtles to link behavioral observations to body size.