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Sperm Cryopreservation as a Tool for Amphibian Conservation: Production of F2 Generation Offspring from Cryo-Produced F1 Progeny

SIMPLE SUMMARY: Assisted reproductive technologies are key components in augmenting captive breeding programs for at-risk amphibian species. Although numerous studies show that these reproductive technologies are successful in generating offspring, there is very little research investigating the rep...

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Autores principales: Lampert, Shaina S., Burger, Isabella J., Julien, Allison R., Gillis, Amanda B., Kouba, Andrew J., Barber, Diane, Kouba, Carrie K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9817882/
https://www.ncbi.nlm.nih.gov/pubmed/36611663
http://dx.doi.org/10.3390/ani13010053
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author Lampert, Shaina S.
Burger, Isabella J.
Julien, Allison R.
Gillis, Amanda B.
Kouba, Andrew J.
Barber, Diane
Kouba, Carrie K.
author_facet Lampert, Shaina S.
Burger, Isabella J.
Julien, Allison R.
Gillis, Amanda B.
Kouba, Andrew J.
Barber, Diane
Kouba, Carrie K.
author_sort Lampert, Shaina S.
collection PubMed
description SIMPLE SUMMARY: Assisted reproductive technologies are key components in augmenting captive breeding programs for at-risk amphibian species. Although numerous studies show that these reproductive technologies are successful in generating offspring, there is very little research investigating the reproductive viability of those individuals. It is crucial to gain insight on the reproductive potential of offspring generated using assisted reproductive technologies when those individuals are to be used for future breeding, or wild release. We report three instances where amphibian offspring generated using a suite of assisted reproductive technologies were monitored through reproductive maturity and subsequently produced an F2 generation. ABSTRACT: Sperm cryopreservation and biobanking are emerging as tools for supporting genetic management of small and threatened populations in amphibian conservation programs. However, there is little to no evidence demonstrating reproductive maturity and viability of offspring generated with cryopreserved sperm, potentially limiting widespread integration of these technologies. The purpose of this report is to demonstrate that amphibian sperm can be cryopreserved and thawed to successfully produce individuals of an F1 generation that can reach adulthood and reproductive maturity, to generating viable gametes and an F2 generation. Species-specific exogenous hormones were administered to both F0 and F1 adults to stimulate spermiation and oviposition in the eastern tiger salamander (Ambystoma tigrinum), dusky gopher frog (Lithobates sevosa), and Puerto Rican crested toad (Peltophryne lemur). Sperm cells collected non-lethally from F0 adults were cryopreserved, thawed, and used for in vitro fertilization (IVF) to produce F1 offspring. Individuals of the F1 generation are shown to reach adulthood, express viable gametes, and produce offspring through facilitated breeding, or IVF. The production of amphibian F2 generations shown here demonstrates that amphibian sperm collected non-lethally can be banked and used to generate reproductively viable animals of subsequent generations, thus maintaining valuable genetic linages and diversity in threatened amphibian species. The incredible value that cryopreservation of sperm has for long-term genetic management aids in the sustainability of both in situ and ex situ conservation efforts for this taxon.
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spelling pubmed-98178822023-01-07 Sperm Cryopreservation as a Tool for Amphibian Conservation: Production of F2 Generation Offspring from Cryo-Produced F1 Progeny Lampert, Shaina S. Burger, Isabella J. Julien, Allison R. Gillis, Amanda B. Kouba, Andrew J. Barber, Diane Kouba, Carrie K. Animals (Basel) Brief Report SIMPLE SUMMARY: Assisted reproductive technologies are key components in augmenting captive breeding programs for at-risk amphibian species. Although numerous studies show that these reproductive technologies are successful in generating offspring, there is very little research investigating the reproductive viability of those individuals. It is crucial to gain insight on the reproductive potential of offspring generated using assisted reproductive technologies when those individuals are to be used for future breeding, or wild release. We report three instances where amphibian offspring generated using a suite of assisted reproductive technologies were monitored through reproductive maturity and subsequently produced an F2 generation. ABSTRACT: Sperm cryopreservation and biobanking are emerging as tools for supporting genetic management of small and threatened populations in amphibian conservation programs. However, there is little to no evidence demonstrating reproductive maturity and viability of offspring generated with cryopreserved sperm, potentially limiting widespread integration of these technologies. The purpose of this report is to demonstrate that amphibian sperm can be cryopreserved and thawed to successfully produce individuals of an F1 generation that can reach adulthood and reproductive maturity, to generating viable gametes and an F2 generation. Species-specific exogenous hormones were administered to both F0 and F1 adults to stimulate spermiation and oviposition in the eastern tiger salamander (Ambystoma tigrinum), dusky gopher frog (Lithobates sevosa), and Puerto Rican crested toad (Peltophryne lemur). Sperm cells collected non-lethally from F0 adults were cryopreserved, thawed, and used for in vitro fertilization (IVF) to produce F1 offspring. Individuals of the F1 generation are shown to reach adulthood, express viable gametes, and produce offspring through facilitated breeding, or IVF. The production of amphibian F2 generations shown here demonstrates that amphibian sperm collected non-lethally can be banked and used to generate reproductively viable animals of subsequent generations, thus maintaining valuable genetic linages and diversity in threatened amphibian species. The incredible value that cryopreservation of sperm has for long-term genetic management aids in the sustainability of both in situ and ex situ conservation efforts for this taxon. MDPI 2022-12-23 /pmc/articles/PMC9817882/ /pubmed/36611663 http://dx.doi.org/10.3390/ani13010053 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Lampert, Shaina S.
Burger, Isabella J.
Julien, Allison R.
Gillis, Amanda B.
Kouba, Andrew J.
Barber, Diane
Kouba, Carrie K.
Sperm Cryopreservation as a Tool for Amphibian Conservation: Production of F2 Generation Offspring from Cryo-Produced F1 Progeny
title Sperm Cryopreservation as a Tool for Amphibian Conservation: Production of F2 Generation Offspring from Cryo-Produced F1 Progeny
title_full Sperm Cryopreservation as a Tool for Amphibian Conservation: Production of F2 Generation Offspring from Cryo-Produced F1 Progeny
title_fullStr Sperm Cryopreservation as a Tool for Amphibian Conservation: Production of F2 Generation Offspring from Cryo-Produced F1 Progeny
title_full_unstemmed Sperm Cryopreservation as a Tool for Amphibian Conservation: Production of F2 Generation Offspring from Cryo-Produced F1 Progeny
title_short Sperm Cryopreservation as a Tool for Amphibian Conservation: Production of F2 Generation Offspring from Cryo-Produced F1 Progeny
title_sort sperm cryopreservation as a tool for amphibian conservation: production of f2 generation offspring from cryo-produced f1 progeny
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9817882/
https://www.ncbi.nlm.nih.gov/pubmed/36611663
http://dx.doi.org/10.3390/ani13010053
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