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Platelet Aggregation, Mitochondrial Function and Morphology in Cold Storage: Impact of Resveratrol and Cytochrome c Supplementation

Donated platelets are critical components of hemostasis management. Extending platelet storage beyond the recommended guidelines (5 days, 22 °C) is of clinical significance. Platelet coagulation function can be prolonged with resveratrol (Res) or cytochrome c (Cyt c) at 4 °C. We hypothesized that st...

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Autores principales: Ekaney, Michael L., Carrillo-Garcia, Juan Carlos, Gonzalez-Gray, Gabrielle, Wilson, Hadley H., Jordan, Mary M., McKillop, Iain H., Evans, Susan L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818067/
https://www.ncbi.nlm.nih.gov/pubmed/36611959
http://dx.doi.org/10.3390/cells12010166
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author Ekaney, Michael L.
Carrillo-Garcia, Juan Carlos
Gonzalez-Gray, Gabrielle
Wilson, Hadley H.
Jordan, Mary M.
McKillop, Iain H.
Evans, Susan L.
author_facet Ekaney, Michael L.
Carrillo-Garcia, Juan Carlos
Gonzalez-Gray, Gabrielle
Wilson, Hadley H.
Jordan, Mary M.
McKillop, Iain H.
Evans, Susan L.
author_sort Ekaney, Michael L.
collection PubMed
description Donated platelets are critical components of hemostasis management. Extending platelet storage beyond the recommended guidelines (5 days, 22 °C) is of clinical significance. Platelet coagulation function can be prolonged with resveratrol (Res) or cytochrome c (Cyt c) at 4 °C. We hypothesized that storage under these conditions is associated with maintained aggregation function, decreased reactive oxygen species (ROS) production, increased mitochondrial respiratory function, and preserved morphology. Donated platelets were stored at 22 °C or 4 °C supplemented with 50 μM Res or 100 μM Cyt c and assayed on days 0 (baseline), 5, 7 and 10 for platelet aggregation, morphology, intracellular ROS, and mitochondrial function. Declining platelet function and increased intracellular ROS were maintained by Res and Cyt c. Platelet respiratory control ratio declined during storage using complex I + II (CI + CII) or CIV substrates. No temperature-dependent differences (4 °C versus 22 °C) in respiratory function were observed. Altered platelet morphology was observed after 7 days at 22 °C, effects that were blunted at 4 °C independent of exposure to Res or Cyt c. Storage of platelets at 4 °C with Res and Cyt c modulates ROS generation and platelet structural integrity.
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spelling pubmed-98180672023-01-07 Platelet Aggregation, Mitochondrial Function and Morphology in Cold Storage: Impact of Resveratrol and Cytochrome c Supplementation Ekaney, Michael L. Carrillo-Garcia, Juan Carlos Gonzalez-Gray, Gabrielle Wilson, Hadley H. Jordan, Mary M. McKillop, Iain H. Evans, Susan L. Cells Article Donated platelets are critical components of hemostasis management. Extending platelet storage beyond the recommended guidelines (5 days, 22 °C) is of clinical significance. Platelet coagulation function can be prolonged with resveratrol (Res) or cytochrome c (Cyt c) at 4 °C. We hypothesized that storage under these conditions is associated with maintained aggregation function, decreased reactive oxygen species (ROS) production, increased mitochondrial respiratory function, and preserved morphology. Donated platelets were stored at 22 °C or 4 °C supplemented with 50 μM Res or 100 μM Cyt c and assayed on days 0 (baseline), 5, 7 and 10 for platelet aggregation, morphology, intracellular ROS, and mitochondrial function. Declining platelet function and increased intracellular ROS were maintained by Res and Cyt c. Platelet respiratory control ratio declined during storage using complex I + II (CI + CII) or CIV substrates. No temperature-dependent differences (4 °C versus 22 °C) in respiratory function were observed. Altered platelet morphology was observed after 7 days at 22 °C, effects that were blunted at 4 °C independent of exposure to Res or Cyt c. Storage of platelets at 4 °C with Res and Cyt c modulates ROS generation and platelet structural integrity. MDPI 2022-12-30 /pmc/articles/PMC9818067/ /pubmed/36611959 http://dx.doi.org/10.3390/cells12010166 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ekaney, Michael L.
Carrillo-Garcia, Juan Carlos
Gonzalez-Gray, Gabrielle
Wilson, Hadley H.
Jordan, Mary M.
McKillop, Iain H.
Evans, Susan L.
Platelet Aggregation, Mitochondrial Function and Morphology in Cold Storage: Impact of Resveratrol and Cytochrome c Supplementation
title Platelet Aggregation, Mitochondrial Function and Morphology in Cold Storage: Impact of Resveratrol and Cytochrome c Supplementation
title_full Platelet Aggregation, Mitochondrial Function and Morphology in Cold Storage: Impact of Resveratrol and Cytochrome c Supplementation
title_fullStr Platelet Aggregation, Mitochondrial Function and Morphology in Cold Storage: Impact of Resveratrol and Cytochrome c Supplementation
title_full_unstemmed Platelet Aggregation, Mitochondrial Function and Morphology in Cold Storage: Impact of Resveratrol and Cytochrome c Supplementation
title_short Platelet Aggregation, Mitochondrial Function and Morphology in Cold Storage: Impact of Resveratrol and Cytochrome c Supplementation
title_sort platelet aggregation, mitochondrial function and morphology in cold storage: impact of resveratrol and cytochrome c supplementation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818067/
https://www.ncbi.nlm.nih.gov/pubmed/36611959
http://dx.doi.org/10.3390/cells12010166
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