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Cervicovaginal-Microbiome Analysis by 16S Sequencing and Real-Time PCR in Patients from Novosibirsk (Russia) with Cervical Lesions and Several Years after Cancer Treatment

Disturbed cervicovaginal-microbiome (CVM) structure promotes human papillomavirus (HPV) persistence and reflects risks of cervical lesions and cancer onset and recurrence. Therefore, microbiomic biomarkers may be useful for cervical disease screening and patient management. Here, by 16S rRNA gene se...

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Autores principales: Ivanov, Mikhail K., Brenner, Evgeny V., Hodkevich, Anastasia A., Dzyubenko, Victoria V., Krasilnikov, Sergey E., Mansurova, Alphiya S., Vakhturova, Irina E., Agletdinov, Eduard F., Shumeikina, Anastasia O., Chernyshova, Alyona L., Titov, Sergei E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818139/
https://www.ncbi.nlm.nih.gov/pubmed/36611432
http://dx.doi.org/10.3390/diagnostics13010140
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author Ivanov, Mikhail K.
Brenner, Evgeny V.
Hodkevich, Anastasia A.
Dzyubenko, Victoria V.
Krasilnikov, Sergey E.
Mansurova, Alphiya S.
Vakhturova, Irina E.
Agletdinov, Eduard F.
Shumeikina, Anastasia O.
Chernyshova, Alyona L.
Titov, Sergei E.
author_facet Ivanov, Mikhail K.
Brenner, Evgeny V.
Hodkevich, Anastasia A.
Dzyubenko, Victoria V.
Krasilnikov, Sergey E.
Mansurova, Alphiya S.
Vakhturova, Irina E.
Agletdinov, Eduard F.
Shumeikina, Anastasia O.
Chernyshova, Alyona L.
Titov, Sergei E.
author_sort Ivanov, Mikhail K.
collection PubMed
description Disturbed cervicovaginal-microbiome (CVM) structure promotes human papillomavirus (HPV) persistence and reflects risks of cervical lesions and cancer onset and recurrence. Therefore, microbiomic biomarkers may be useful for cervical disease screening and patient management. Here, by 16S rRNA gene sequencing and commercial PCR-based diagnostic kits, we profiled CVM in cytological preparations from 140 HPV-tested women (from Novosibirsk, Russia) with normal cytological findings, cervical lesions, or cancer and from 101 women who had recently received different cancer therapies. An increase in lesion severity was accompanied by higher HPV prevalence and elevated CVM biodiversity. Post-treatment CVM was found to be enriched with well-known microbial biomarkers of dysbiosis, just as in cervical disease. Nonetheless, concentrations of some skin-borne and environmental species (which gradually increased with increasing lesion severity)—especially Cutibacterium spp., Achromobacter spp., and Ralstonia pickettii—was low in post-treatment patients and depended on treatment types. Frequency of Lactobacillus iners dominance was high in all groups and depended on treatment types in post-treatment patients. Microbiome analysis via PCR-based kits revealed statistically significant differences among all groups of patients. Thus, microbiome profiling may help to find diagnostic and prognostic markers for management of cervical lesions; quantitative PCR-based kits may be suitable for these purposes.
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spelling pubmed-98181392023-01-07 Cervicovaginal-Microbiome Analysis by 16S Sequencing and Real-Time PCR in Patients from Novosibirsk (Russia) with Cervical Lesions and Several Years after Cancer Treatment Ivanov, Mikhail K. Brenner, Evgeny V. Hodkevich, Anastasia A. Dzyubenko, Victoria V. Krasilnikov, Sergey E. Mansurova, Alphiya S. Vakhturova, Irina E. Agletdinov, Eduard F. Shumeikina, Anastasia O. Chernyshova, Alyona L. Titov, Sergei E. Diagnostics (Basel) Article Disturbed cervicovaginal-microbiome (CVM) structure promotes human papillomavirus (HPV) persistence and reflects risks of cervical lesions and cancer onset and recurrence. Therefore, microbiomic biomarkers may be useful for cervical disease screening and patient management. Here, by 16S rRNA gene sequencing and commercial PCR-based diagnostic kits, we profiled CVM in cytological preparations from 140 HPV-tested women (from Novosibirsk, Russia) with normal cytological findings, cervical lesions, or cancer and from 101 women who had recently received different cancer therapies. An increase in lesion severity was accompanied by higher HPV prevalence and elevated CVM biodiversity. Post-treatment CVM was found to be enriched with well-known microbial biomarkers of dysbiosis, just as in cervical disease. Nonetheless, concentrations of some skin-borne and environmental species (which gradually increased with increasing lesion severity)—especially Cutibacterium spp., Achromobacter spp., and Ralstonia pickettii—was low in post-treatment patients and depended on treatment types. Frequency of Lactobacillus iners dominance was high in all groups and depended on treatment types in post-treatment patients. Microbiome analysis via PCR-based kits revealed statistically significant differences among all groups of patients. Thus, microbiome profiling may help to find diagnostic and prognostic markers for management of cervical lesions; quantitative PCR-based kits may be suitable for these purposes. MDPI 2023-01-01 /pmc/articles/PMC9818139/ /pubmed/36611432 http://dx.doi.org/10.3390/diagnostics13010140 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ivanov, Mikhail K.
Brenner, Evgeny V.
Hodkevich, Anastasia A.
Dzyubenko, Victoria V.
Krasilnikov, Sergey E.
Mansurova, Alphiya S.
Vakhturova, Irina E.
Agletdinov, Eduard F.
Shumeikina, Anastasia O.
Chernyshova, Alyona L.
Titov, Sergei E.
Cervicovaginal-Microbiome Analysis by 16S Sequencing and Real-Time PCR in Patients from Novosibirsk (Russia) with Cervical Lesions and Several Years after Cancer Treatment
title Cervicovaginal-Microbiome Analysis by 16S Sequencing and Real-Time PCR in Patients from Novosibirsk (Russia) with Cervical Lesions and Several Years after Cancer Treatment
title_full Cervicovaginal-Microbiome Analysis by 16S Sequencing and Real-Time PCR in Patients from Novosibirsk (Russia) with Cervical Lesions and Several Years after Cancer Treatment
title_fullStr Cervicovaginal-Microbiome Analysis by 16S Sequencing and Real-Time PCR in Patients from Novosibirsk (Russia) with Cervical Lesions and Several Years after Cancer Treatment
title_full_unstemmed Cervicovaginal-Microbiome Analysis by 16S Sequencing and Real-Time PCR in Patients from Novosibirsk (Russia) with Cervical Lesions and Several Years after Cancer Treatment
title_short Cervicovaginal-Microbiome Analysis by 16S Sequencing and Real-Time PCR in Patients from Novosibirsk (Russia) with Cervical Lesions and Several Years after Cancer Treatment
title_sort cervicovaginal-microbiome analysis by 16s sequencing and real-time pcr in patients from novosibirsk (russia) with cervical lesions and several years after cancer treatment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818139/
https://www.ncbi.nlm.nih.gov/pubmed/36611432
http://dx.doi.org/10.3390/diagnostics13010140
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