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Mechanism Underlying Light Intensity-Induced Melanin Synthesis of Auricularia heimuer Revealed by Transcriptome Analysis

Auricularia heimuer is a traditional edible and medicinal mushroom, which is widely used in biochemical research and is regarded as a good dietary supplement. The color of the ear-like fruiting body is an important indicator of its commercial quality. However, the mechanism by which light intensity...

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Autores principales: Qiu, Zhiheng, Gao, Yanliang, Wang, Shuang, Wang, Jun, Wang, Xinyi, Cai, Nuo, Zhao, Jiazhi, Li, Tingshu, Li, Hongpeng, Li, Tianlai, Shu, Lili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818193/
https://www.ncbi.nlm.nih.gov/pubmed/36611851
http://dx.doi.org/10.3390/cells12010056
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author Qiu, Zhiheng
Gao, Yanliang
Wang, Shuang
Wang, Jun
Wang, Xinyi
Cai, Nuo
Zhao, Jiazhi
Li, Tingshu
Li, Hongpeng
Li, Tianlai
Shu, Lili
author_facet Qiu, Zhiheng
Gao, Yanliang
Wang, Shuang
Wang, Jun
Wang, Xinyi
Cai, Nuo
Zhao, Jiazhi
Li, Tingshu
Li, Hongpeng
Li, Tianlai
Shu, Lili
author_sort Qiu, Zhiheng
collection PubMed
description Auricularia heimuer is a traditional edible and medicinal mushroom, which is widely used in biochemical research and is regarded as a good dietary supplement. The color of the ear-like fruiting body is an important indicator of its commercial quality. However, the mechanism by which light intensity influences the melanin synthesis of A. heimuer remains unclear. Here, we show that fruiting body color is significantly affected by light intensity. Transcriptional profiles of the fruiting bodies of A. heimuer grown in different light intensities were further analyzed. More differentially expressed genes (DEGs) were identified with a greater light intensity difference. A total of 1388 DEGs were identified from six comparisons, including 503 up-regulated genes and 885 down-regulated genes. The up-regulated genes were mainly associated with light sensing via photoreceptors, signal transduction via the mitogen-activated protein kinase (MAPK) signaling pathway, and melanin synthesis via the tyrosine metabolic pathway. Therefore, the genes involved in these processes may participate in regulating melanin synthesis under high light intensity. This insight into the transcriptional regulation of A. heimuer to light intensity should help to further comprehensively elucidate the underlying mechanism of light-induced melanin synthesis.
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spelling pubmed-98181932023-01-07 Mechanism Underlying Light Intensity-Induced Melanin Synthesis of Auricularia heimuer Revealed by Transcriptome Analysis Qiu, Zhiheng Gao, Yanliang Wang, Shuang Wang, Jun Wang, Xinyi Cai, Nuo Zhao, Jiazhi Li, Tingshu Li, Hongpeng Li, Tianlai Shu, Lili Cells Article Auricularia heimuer is a traditional edible and medicinal mushroom, which is widely used in biochemical research and is regarded as a good dietary supplement. The color of the ear-like fruiting body is an important indicator of its commercial quality. However, the mechanism by which light intensity influences the melanin synthesis of A. heimuer remains unclear. Here, we show that fruiting body color is significantly affected by light intensity. Transcriptional profiles of the fruiting bodies of A. heimuer grown in different light intensities were further analyzed. More differentially expressed genes (DEGs) were identified with a greater light intensity difference. A total of 1388 DEGs were identified from six comparisons, including 503 up-regulated genes and 885 down-regulated genes. The up-regulated genes were mainly associated with light sensing via photoreceptors, signal transduction via the mitogen-activated protein kinase (MAPK) signaling pathway, and melanin synthesis via the tyrosine metabolic pathway. Therefore, the genes involved in these processes may participate in regulating melanin synthesis under high light intensity. This insight into the transcriptional regulation of A. heimuer to light intensity should help to further comprehensively elucidate the underlying mechanism of light-induced melanin synthesis. MDPI 2022-12-23 /pmc/articles/PMC9818193/ /pubmed/36611851 http://dx.doi.org/10.3390/cells12010056 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Qiu, Zhiheng
Gao, Yanliang
Wang, Shuang
Wang, Jun
Wang, Xinyi
Cai, Nuo
Zhao, Jiazhi
Li, Tingshu
Li, Hongpeng
Li, Tianlai
Shu, Lili
Mechanism Underlying Light Intensity-Induced Melanin Synthesis of Auricularia heimuer Revealed by Transcriptome Analysis
title Mechanism Underlying Light Intensity-Induced Melanin Synthesis of Auricularia heimuer Revealed by Transcriptome Analysis
title_full Mechanism Underlying Light Intensity-Induced Melanin Synthesis of Auricularia heimuer Revealed by Transcriptome Analysis
title_fullStr Mechanism Underlying Light Intensity-Induced Melanin Synthesis of Auricularia heimuer Revealed by Transcriptome Analysis
title_full_unstemmed Mechanism Underlying Light Intensity-Induced Melanin Synthesis of Auricularia heimuer Revealed by Transcriptome Analysis
title_short Mechanism Underlying Light Intensity-Induced Melanin Synthesis of Auricularia heimuer Revealed by Transcriptome Analysis
title_sort mechanism underlying light intensity-induced melanin synthesis of auricularia heimuer revealed by transcriptome analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818193/
https://www.ncbi.nlm.nih.gov/pubmed/36611851
http://dx.doi.org/10.3390/cells12010056
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