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Association of High Immunohistochemical Expression of Minichromosome Maintenance 3 with Human Oral Squamous Cell Carcinoma—A Preliminary Study

Background: Oral squamous cell carcinoma (OSCC) may arise from premalignant oral lesions (PMOL) in most cases. Minichromosome maintenance 3 (MCM3) is a proliferative marker that has been investigated as a potential diagnostic biomarker in the diagnosis of oral cancer. Objectives: To evaluate the ass...

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Autores principales: Zahir, Rabia, Khan, Zafar Ali, Aleem, Benish, Ahmad, Shahzad, Ali, Asif, Issrani, Rakhi, Alruwaili, Mohammed Katib, Iqbal, Shazia, Alghumaiz, Shmoukh Fahad, Alanazi, Sarah Hatab, Umer, Muhammad Farooq, Ullah, Ihsan, Sumera, Ganji, Kiran Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818330/
https://www.ncbi.nlm.nih.gov/pubmed/36611359
http://dx.doi.org/10.3390/diagnostics13010061
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author Zahir, Rabia
Khan, Zafar Ali
Aleem, Benish
Ahmad, Shahzad
Ali, Asif
Issrani, Rakhi
Alruwaili, Mohammed Katib
Iqbal, Shazia
Alghumaiz, Shmoukh Fahad
Alanazi, Sarah Hatab
Umer, Muhammad Farooq
Ullah, Ihsan
Sumera,
Ganji, Kiran Kumar
author_facet Zahir, Rabia
Khan, Zafar Ali
Aleem, Benish
Ahmad, Shahzad
Ali, Asif
Issrani, Rakhi
Alruwaili, Mohammed Katib
Iqbal, Shazia
Alghumaiz, Shmoukh Fahad
Alanazi, Sarah Hatab
Umer, Muhammad Farooq
Ullah, Ihsan
Sumera,
Ganji, Kiran Kumar
author_sort Zahir, Rabia
collection PubMed
description Background: Oral squamous cell carcinoma (OSCC) may arise from premalignant oral lesions (PMOL) in most cases. Minichromosome maintenance 3 (MCM3) is a proliferative marker that has been investigated as a potential diagnostic biomarker in the diagnosis of oral cancer. Objectives: To evaluate the association of MCM3 expression, its clinicopathologic parameters and to identify snuff (also called naswar) as a potential risk factor for changes in MCM3 expression in PMOL and OSCC. Methodology: Immunohistochemistry (IHC) of MCM3 was performed on 32 PMOL, 32 OSCC and 16 normal controls after optimization of IHC methodology. Histoscore (0–300) was used as a scoring system and seven different cut-offs were identified for analyses. Data were analyzed using various statistical tests. Results: Among the seven cutoffs, 40% strong positive cells were found to be a better cut-off as they were associated with many pathological variables (Broder’s grade, Aneroth’s grade, and mitotic activity). The differential MCM3 expression in oral lesions (PMOL and OSCC) was statistically significant (p = 0.03). Moreover, MCM3 expression is raised with increased duration and frequency of snuff use. Conclusion: High MCM3 expression is associated with disease progression and is a potential indicator of malignant transformations from PMOL to OSCC. Moreover, the use of snuff is associated with MCM3 over-expression.
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spelling pubmed-98183302023-01-07 Association of High Immunohistochemical Expression of Minichromosome Maintenance 3 with Human Oral Squamous Cell Carcinoma—A Preliminary Study Zahir, Rabia Khan, Zafar Ali Aleem, Benish Ahmad, Shahzad Ali, Asif Issrani, Rakhi Alruwaili, Mohammed Katib Iqbal, Shazia Alghumaiz, Shmoukh Fahad Alanazi, Sarah Hatab Umer, Muhammad Farooq Ullah, Ihsan Sumera, Ganji, Kiran Kumar Diagnostics (Basel) Article Background: Oral squamous cell carcinoma (OSCC) may arise from premalignant oral lesions (PMOL) in most cases. Minichromosome maintenance 3 (MCM3) is a proliferative marker that has been investigated as a potential diagnostic biomarker in the diagnosis of oral cancer. Objectives: To evaluate the association of MCM3 expression, its clinicopathologic parameters and to identify snuff (also called naswar) as a potential risk factor for changes in MCM3 expression in PMOL and OSCC. Methodology: Immunohistochemistry (IHC) of MCM3 was performed on 32 PMOL, 32 OSCC and 16 normal controls after optimization of IHC methodology. Histoscore (0–300) was used as a scoring system and seven different cut-offs were identified for analyses. Data were analyzed using various statistical tests. Results: Among the seven cutoffs, 40% strong positive cells were found to be a better cut-off as they were associated with many pathological variables (Broder’s grade, Aneroth’s grade, and mitotic activity). The differential MCM3 expression in oral lesions (PMOL and OSCC) was statistically significant (p = 0.03). Moreover, MCM3 expression is raised with increased duration and frequency of snuff use. Conclusion: High MCM3 expression is associated with disease progression and is a potential indicator of malignant transformations from PMOL to OSCC. Moreover, the use of snuff is associated with MCM3 over-expression. MDPI 2022-12-26 /pmc/articles/PMC9818330/ /pubmed/36611359 http://dx.doi.org/10.3390/diagnostics13010061 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zahir, Rabia
Khan, Zafar Ali
Aleem, Benish
Ahmad, Shahzad
Ali, Asif
Issrani, Rakhi
Alruwaili, Mohammed Katib
Iqbal, Shazia
Alghumaiz, Shmoukh Fahad
Alanazi, Sarah Hatab
Umer, Muhammad Farooq
Ullah, Ihsan
Sumera,
Ganji, Kiran Kumar
Association of High Immunohistochemical Expression of Minichromosome Maintenance 3 with Human Oral Squamous Cell Carcinoma—A Preliminary Study
title Association of High Immunohistochemical Expression of Minichromosome Maintenance 3 with Human Oral Squamous Cell Carcinoma—A Preliminary Study
title_full Association of High Immunohistochemical Expression of Minichromosome Maintenance 3 with Human Oral Squamous Cell Carcinoma—A Preliminary Study
title_fullStr Association of High Immunohistochemical Expression of Minichromosome Maintenance 3 with Human Oral Squamous Cell Carcinoma—A Preliminary Study
title_full_unstemmed Association of High Immunohistochemical Expression of Minichromosome Maintenance 3 with Human Oral Squamous Cell Carcinoma—A Preliminary Study
title_short Association of High Immunohistochemical Expression of Minichromosome Maintenance 3 with Human Oral Squamous Cell Carcinoma—A Preliminary Study
title_sort association of high immunohistochemical expression of minichromosome maintenance 3 with human oral squamous cell carcinoma—a preliminary study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818330/
https://www.ncbi.nlm.nih.gov/pubmed/36611359
http://dx.doi.org/10.3390/diagnostics13010061
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